Metabolic adaptation drives self-organization during skin organoid morphogenesis DOI Creative Commons
Mingxing Lei, Jingwei Jiang, Weiwei Liu

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 23, 2024

Abstract Self-organization in organoid morphogenesis involves the coordinated arrangement of interacting cells into higher-order structures, yet underlying principles remain elusive. Here, we investigate how epidermal and dermal respond distinctively to elevated levels hypoxia during skin that largely resembles development embryogenesis. We unveil autonomously generated hypoxic environment-induced metabolic adaptation drives transition from coalesced spheroids a planarized structure organoids through following three levels. Hif1a-mediated anaerobic metabolism positions liquid phase cultures under lower oxygen level, facilitating tissue separation layer layer. Hypoxia-driven activation lysosomal hydrolases eliminates suprabasal keratin debris planar epidermis formation. Fibroblasts adjacent basal have differential hypoxia, which exhibit enhanced retinoid become putative papillary dermis. Together, these hypoxia-induced adaptations contribute reconstructing architecture similar physiological development. Our findings highlight ability alteration trigger varied cellular responses, leading self-organizing spheroids-to-planar topological transformations restoration homeostasis.

Язык: Английский

Patterning and folding of intestinal villi by active mesenchymal dewetting DOI Creative Commons
Tyler R. Huycke, Teemu J. Häkkinen,

Hikaru Miyazaki

и другие.

Cell, Год журнала: 2024, Номер 187(12), С. 3072 - 3089.e20

Опубликована: Май 22, 2024

Tissue folds are structural motifs critical to organ function. In the intestine, bending of a flat epithelium into periodic pattern gives rise villi, finger-like protrusions that enable nutrient absorption. However, molecular and mechanical processes driving villus morphogenesis remain unclear. Here, we identify an active mechanism simultaneously patterns intestinal initiate formation. At cellular level, find PDGFRA+ subepithelial mesenchymal cells generate myosin II-dependent forces sufficient produce patterned curvature in neighboring tissue interfaces. This symmetry-breaking process requires altered cell extracellular matrix interactions enabled by metalloproteinase-mediated fluidization. Computational models, together with vitro vivo experiments, revealed these features manifest at level as differences interfacial tensions promote aggregation interface through analogous dewetting thin liquid film.

Язык: Английский

Процитировано

26
The Caenorhabditis elegans cuticle and precuticle: a model for studying dynamic apical extracellular matrices in vivo DOI Creative Commons
Meera V. Sundaram, Nathalie Pujol

Genetics, Год журнала: 2024, Номер 227(4)

Опубликована: Июль 12, 2024

Apical extracellular matrices (aECMs) coat the exposed surfaces of animal bodies to shape tissues, influence social interactions, and protect against pathogens other environmental challenges. In nematode Caenorhabditis elegans, collagenous cuticle zona pellucida protein-rich precuticle aECMs alternately external epithelia across molt cycle play many important roles in worm's development, behavior, physiology. Both these types contain matrix proteins related those vertebrates, as well some that are nematode-specific. Extensive differences observed among tissues life stages demonstrate a major feature epithelial cell identity. addition forming discrete layers, components assemble into complex substructures such ridges, furrows, nanoscale pillars. The epidermis mechanically linked, allowing sense damage induce protective innate immune stress responses. C. elegans model, with its optical transparency, facilitates study aECM biology structure/function relationships all myriad ways by which can an organism.

Язык: Английский

Процитировано

15
Stiffness anisotropy coordinates supracellular contractility driving long-range myotube-ECM alignment DOI Creative Commons
Nathaniel P. Skillin, Bruce E. Kirkpatrick, Katie M. Herbert

и другие.

Science Advances, Год журнала: 2024, Номер 10(22)

Опубликована: Май 31, 2024

The ability of cells to organize into tissues with proper structure and function requires the effective coordination proliferation, migration, polarization, differentiation across length scales. Skeletal muscle is innately anisotropic; however, few biomaterials can emulate mechanical anisotropy determine its influence on tissue patterning without introducing confounding topography. Here, we demonstrate that substrate stiffness coordinates contractility-driven collective cellular dynamics resulting in C2C12 myotube alignment over millimeter-scale distances. When cultured mechanically anisotropic liquid crystalline polymer networks (LCNs) lacking topography, myoblasts collectively polarize stiffest direction. Cellular amplified through reciprocal cell-ECM emerge during fusion, driving global myotube-ECM ordering. Conversely, was restricted small local domains no directional preference isotropic LCNs same chemical formulation. These findings provide valuable insights for designing mimic microenvironments underscore importance orchestrating morphogenesis.

Язык: Английский

Процитировано

10
Embryonic feather bud development – a keystone model for vertebrate organogenesis DOI
Jon Riddell, Denis J. Headon

Developmental Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1
Measuring and manipulating mechanical forces during development DOI
Clémentine Villeneuve, Kaitlin P. McCreery, Sara A. Wickström

и другие.

Nature Cell Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Язык: Английский

Процитировано

1
A tension-induced morphological transition shapes the avian extra-embryonic territory DOI Creative Commons
Arthur Michaut, Alexander Chamolly, Aurélien Villedieu

и другие.

Current Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

1
Self-organized patterning of crocodile head scales by compressive folding DOI Creative Commons
Gabriel N. Santos-Durán, Rory L. Cooper, Ebrahim Jahanbakhsh

и другие.

Nature, Год журнала: 2024, Номер unknown

Опубликована: Дек. 11, 2024

Amniote integumentary appendages constitute a diverse group of micro-organs, including feathers, hair and scales. These structures typically develop as genetically controlled units

Язык: Английский

Процитировано

5
Emergence and properties of adult mammalian epidermal stem cells DOI Creative Commons
Purna Gadre,

Pavlina Markova,

Mirsana P. Ebrahimkutty

и другие.

Developmental Biology, Год журнала: 2024, Номер 515, С. 129 - 138

Опубликована: Июль 24, 2024

In this review we discuss how the mammalian interfollicular epidermis forms during development, maintains homeostasis, and is repaired following wounding. Recent studies have provided new insights into relationship between stem cell compartment differentiating layers; ability of differentiated cells to dedifferentiate cells; epigenetic memory epidermal

Язык: Английский

Процитировано

4
Global morphogenesis regulating tissue architecture and organogenesis DOI Creative Commons
Ugo Ripamonti

Biomaterials Advances, Год журнала: 2025, Номер 172, С. 214262 - 214262

Опубликована: Март 2, 2025

Язык: Английский

Процитировано

0
Interfacial energy constraints are sufficient to align cells over large distances DOI Creative Commons
Sham Tlili, Murat Shagirov, Shaobo Zhang

и другие.

Biophysical Journal, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0