Scientific Reports,
Год журнала:
2023,
Номер
13(1)
Опубликована: Июль 24, 2023
Hydrogels
have
been
utilized
extensively
as
a
material
for
retaining
position
information
in
tissue
imaging
procedures,
such
clearing
and
super-resolution
imaging.
Immunostaining
thick
biological
tissues,
however,
poses
bottleneck
that
restricts
sample
size.
The
recently
developed
technique
known
entangled
link-augmented
stretchable
tissue-hydrogel
(ELAST)
accelerates
the
immunostaining
process
by
embedding
specimens
long-chain
polymers
stretching
them.
A
more
advanced
version
of
ELAST,
magnifiable
(mELAST),
achieves
rapid
expansion
neutral
subsequently
hydrolyzing
Building
on
these
techniques,
we
introduce
variant
mELAST
called
ExELAST.
This
approach
uses
charged
monomers
to
stretch
expand
slices.
Using
ExELAST,
first
tested
two
hydrogel
compositions
could
permit
uniform
specimens.
Then,
apply
tailored
500-μm-thick
mouse
brain
slices
demonstrated
they
can
be
stained
within
days
imaged
with
resolution
below
diffraction
limit
light.
Combining
transparent
embedding
with
sectioning
is
likely
to
be
the
future
direction
for
tissue
clearing
and
3-dimensional
(3D)
imaging.
A
newly
published
system,
TESOS
(Transparent
Embedding
Solvent
System),
ensures
consistent
submicron
resolution
imaging
throughout
entire
sample,
can
compatible
different
microscopy
systems.
This
method
shows
great
potential
in
connectome
mapping,
might
an
optimal
option
3D
multiplex
immunofluorescence
RNA
situ
hybridization
Additional
efforts
would
needed
innovate
labeling,
imaging,
data
processing
strategies
fully
utilize
of
systems
high-resolution
large-scale
samples.
Abstract
Nanoparticles
are
widely
used
in
biomedical
applications
due
to
their
small
size,
large
surface
area,
and
unique
physicochemical
properties.
These
characteristics
make
them
ideal
for
drug
delivery,
diagnostic
imaging,
therapeutic
interventions.
Their
ability
interact
with
biological
systems
at
the
cellular
molecular
levels
enables
targeted
treatments.
Understanding
biodistribution
of
nanoparticles
level
within
whole
organisms
is
crucial
assessing
safety
effectiveness;
however,
proper
technologies
have
been
lacking
achieve
this.
For
example,
traditional
imaging
techniques
like
magnetic
resonance
(MRI)
computed
tomography
(CT)
often
lack
resolution
needed,
while
tissue‐section‐based
methods
miss
whole‐body
systemic
view.
Recent
tissue
clearing
emerged
as
a
promising
solution
3D
visualization
entire
organs,
they
enable
cellular‐level
without
need
sectioning.
This
review
explores
advancements
diverse
application
studying
nanoparticle
biodistribution,
providing
insights
development
nanoparticle‐based
therapies.
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 14, 2025
Alzheimer's
disease
(AD)
is
a
progressive
and
irreversible
brain
disorder
with
extensive
neuronal
loss
in
the
neocortex
hippocampus.
Current
therapeutic
interventions
focus
on
early
stage
of
AD
but
lack
effective
treatment
for
late
AD,
largely
due
to
inability
replenish
lost
neurons
repair
broken
neural
circuits.
In
this
study,
by
using
engineered
adeno-associated
virus
vectors
that
efficiently
cross
blood-brain-barrier
mouse
brain,
brain-wide
neuroregenerative
gene
therapy
developed
directly
convert
endogenous
astrocytes
into
functional
model
AD.
It
found
≈500
000
new
are
regenerated
widely
distributed
cerebral
cortex
Importantly,
it
demonstrated
converted
can
integrate
pre-existing
networks
improve
various
cognitive
performances
mice.
Chemogenetic
inhibition
abolishes
memory
enhancement
mice,
suggesting
pivotal
role
newly
restoration.
Together,
may
provide
viable
strategy
other
disorders
associated
massive
loss.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 5, 2024
Optical
nanoscopy
of
intact
biological
specimens
has
been
transformed
by
recent
advancements
in
hydrogel-based
tissue
clearing
and
expansion,
enabling
the
imaging
cellular
subcellular
structures
with
molecular
contrast.
However,
existing
high-resolution
fluorescence
microscopes
have
limited
depth,
which
prevents
study
whole-mount
without
physical
sectioning.
To
address
this
challenge,
we
developed
"photochemical
sectioning,"
a
spatially
precise,
light-based
sample
sectioning
process.
By
combining
photochemical
volumetric
lattice
light-sheet
petabyte-scale
computation,
imaged
reconstructed
axons
myelination
sheaths
across
entire
mouse
olfactory
bulbs
at
nanoscale
resolution.
An
olfactory-bulb-wide
analysis
myelinated
unmyelinated
revealed
distinctive
patterns
axon
degeneration
de-/dysmyelination
neurodegenerative
mouse,
highlighting
potential
for
peta-
to
exabyte-scale
super-resolution
studies
using
approach.
