Evolution and host-specific adaptation ofPseudomonas aeruginosa

Science, Год журнала: 2024, Номер 385(6704)

Опубликована: Июль 4, 2024

The major human bacterial pathogen

Язык: Английский

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Distribution of the four type VI secretion systems in Pseudomonas aeruginosa and classification of their core and accessory effectors

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 21, 2025

Abstract Bacterial type VI secretion systems (T6SSs) are puncturing molecular machines that transport effector proteins to kill microbes, manipulate eukaryotic cells, or facilitate nutrient uptake. How and why T6SS effectors differ within a species is not fully understood. Here, we applied population genetics the T6SSs in global of opportunistic pathogen Pseudomonas aeruginosa . We reveal varying occurrence up four distinct machines. Moreover, define conserved core effectors, likely critical for biology P. , accessory can exhibit mutual exclusivity between strains. By ancestral reconstruction, observed dynamic changes gain loss genes species’ evolutionary history. Our work highlights potential importance intraspecific diversity bacterial ecology evolution.

Язык: Английский

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Targeting Acinetobacter baumannii resistance-nodulation-division efflux pump transcriptional regulators to combat antimicrobial resistance

npj Antimicrobials and Resistance, Год журнала: 2025, Номер 3(1)

Опубликована: Янв. 25, 2025

Regulatory elements controlling gene expression fine-tune bacterial responses to environmental cues, including antimicrobials, optimize survival. Acinetobacter baumannii, a pathogen notorious for antimicrobial resistance, relies on efficient efflux systems. Though the role of systems in antibiotic expulsion are well recognized, regulatory mechanisms their remain understudied. This review explores current understanding these regulators, aiming inspire strategies combat resistance and improve therapeutic outcomes.

Язык: Английский

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Genomic and phenotypic characterisation of Pseudomonas aeruginosa isolates from canine otitis externa reveals high-risk sequence types identical to those found in human nosocomial infections

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Фев. 24, 2025

Canine otitis externa (OE) is a frequently-diagnosed condition in veterinary practices worldwide. Pseudomonas aeruginosa commonly associated with chronic and recalcitrant canine OE, but studies detailed genomic phenotypic characterisation of clinical isolates are lacking. OE (n = 253) were collected from different geographical locations Europe characterised respect to antimicrobial resistance biofilm formation. A subset 35) genome sequenced then diversity, complement virulence, resistance, biofilm-associated genes. Genome-sequenced P. strains distributed among phylogroups, showing no obvious clonality. However, two belonged ST111 ST244 respectively,-MLST sequence types AMR nosocomial infections humans. Resistance fluoroquinolones was detected 25% isolates, multidrug 1.6%, though this did not always correlate the presence Additionally, 82% as forming strong biofilms. For first time, study has large multinational collection combination whole sequencing, screening bioinformatic analysis. These cluster together based on diversity or virulence gene complement, supporting their likely environmental origin. identification ST244, important 'high-risk' types, could suggest potential spread between humans dogs. Furthermore, we found that most formed biofilms, exhibited significant level towards critically antimicrobials. findings assist selection appropriate treatments for well possibly identifying one health risks these cohabiting pets

Язык: Английский

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Complex exchanges among plasmids and clonal expansion of lineages shape the population structure and virulence of Borrelia burgdorferi

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 30, 2025

In the United States, Borrelia burgdorferi ( Bb ) is principal etiologic agent of Lyme disease. The complex structure genomes has posed challenges for genomic studies because homology among bacterium's many plasmids, which account ∼40% genome by length, made them difficult to sequence and assemble. We used long-read sequencing generate near-complete assemblies 62 isolates human-derived collected public with plasmid sequences. characterized genetic diversity population in resulting set 82 plasmid-complete sensu stricto genomes. core encoded a chromosome conserved plasmids cp26, lp54, lp17; accessory all other distal arm chromosome. Near-complete reveal that most granular genotypes are clonal expansions rearrangements elements. Ribosomal spacer types (RST) represent multiple collections such genotypes, whereas OspC usually clonal. Structural non-randomly distributed throughout genome, cp32 undergoing dense exchanges linear except sharing blocks themselves type A strains, known possess greater virulence humans, distinguished presence lp28-1 lp56. Rearrangements tended preserve gene content, suggesting functional constraints networks. Using k-partite graph decompositions, we identified sets correlation patterns suggestive modules. Long-read results from expansion lineages have undergone plasmid-encoded Genetic preserved genes even when occur, selection epistatic loci maintains analysis assembled using methods advances our understanding biology disease pathogenesis providing first detailed view variation previously inaccessible areas genome.

Язык: Английский

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Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 9, 2025

Abstract Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen able to cause life- and sight-threating infections. Once considered an extracellular pathogen, numerous studies have shown it can survive intracellularly. Previously, we showed that P. inside cells diversify into distinct subpopulations in vacuoles the cytoplasm. Here, report transition from cytoplasm requires collaboration with subpopulation, through Ca 2+ influx enabled by their type III secretion system (T3SS) translocon pore proteins. Moreover, show among contribute disseminating intracellular bacteria vivo mouse infection model. This study provides basis for future investigate how cooperation of within host may disease progression persistence.

Язык: Английский

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Intracellular Pseudomonas aeruginosa persist and evade antibiotic treatment in a wound infection model

PLoS Pathogens, Год журнала: 2025, Номер 21(2), С. e1012922 - e1012922

Опубликована: Фев. 13, 2025

Persistent bacterial infections evade host immunity and resist antibiotic treatments through various mechanisms that are difficult to evaluate in a living host. Pseudomonas aeruginosa is main cause of chronic patients with cystic fibrosis (CF) wounds. Here, by immersing wounded zebrafish embryos suspension P. isolates from CF patients, we established model persistent infection mimics murine skin model. Live electron microscopy revealed persisting aggregated inside cells, including macrophages, at unprecedented resolution. exhibited adaptive resistance several antibiotics, cell permeable drugs being the most efficient. Moreover, bacteria could be partly re-sensitized antibiotics upon addition anti-biofilm molecules dispersed aggregates vivo . Collectively, this study demonstrates an intracellular location protects innate provides new insights into efficient against infections.

Язык: Английский

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Outbreak investigations of contact patients and the hospital environment after detection of carbapenemase-producing Pseudomonas aeruginosa on general hospital wards

Journal of Hospital Infection, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

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Carbapenem-resistant Morganella morganii carrying bla _KPC-2 or bla _NDM-1 in the clinic: one-decade genomic epidemiology analysis

Microbiology Spectrum, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

ABSTRACT Carbapenem-resistant Morganella morganii (CRMM) isolates, particularly those producing Klebsiella pneumoniae carbapenemase-2 (KPC-2) or New Delhi metallo-β-lactamase-1 (NDM-1), are increasingly being recognized as causative agents of nosocomial infections. However, systematic phylogeography and genetic characterization these isolates worldwide still lacking. Here, through seven years surveillance CRMM in a tertiary hospital, we analyzed the genomic characteristics bla KPC-2 - NDM-1 -positive isolates. Furthermore, conducted global epidemiological study spp. harboring KPC NDM using NCBI database over past decade. By combining timeline isolate collection with structural analysis plasmids, traced evolution IncL/M plasmid, which acquired gene. Our findings indicate that horizontal transfer Tn 6296 based on IS 26 is crucial for transmission Additionally, 125 transposon appears to have played an important role early plasmid-mediated dissemination ; however, it has been surpassed recent by other elements, including CR . In summary, phylogeographic globally, elucidated their spatial–temporal distribution revealed evolutionary KPC- NDM-producing predominant "epidemic" clone. IMPORTANCE Currently, infections attributable carbapenem-resistant rise, highlighting increasing severity antimicrobial resistance. this study, trajectory genes within core environments. We emphasize necessity strengthening controlling organisms clinical settings prevent generation so-called "superbug"

Язык: Английский

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Spatial transcriptomics identifies novel Pseudomonas aeruginosa virulence factors

Cell Genomics, Год журнала: 2025, Номер 5(3), С. 100805 - 100805

Опубликована: Март 1, 2025

Highlights•Spatial transcriptome analysis captures host and pathogen signatures•Integrating spatial data allows the prediction of bacterial burden at infected sites•Transcript enrichment highlights virulence mechanismsSummaryTo examine host-pathogen interactions, we leveraged a dual transcriptomics approach that simultaneously expression Pseudomonas aeruginosa genes alongside entire using murine model ocular infection. This method revealed differential pathogen- host-specific gene patterns in corneas, which generated unified transcriptional map By integrating these data, developed predictive ridge regression trained on images from tissues. The achieved an R2 score 0.923 predicting distributions identifying novel biomarkers associated with disease severity. Among iron acquisition pathogen-specific transcripts showed significant interface, discovered mediator PA2590, was required for virulence. study therefore power combining to uncover complex interactions identify potentially druggable targets.Graphical abstract

Язык: Английский

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