Frontiers in Cellular and Infection Microbiology,
Год журнала:
2024,
Номер
14
Опубликована: Авг. 22, 2024
The
chronic
airway
infections
with
Pseudomonas
aeruginosa
are
the
major
co-morbidity
in
people
cystic
fibrosis
(CF).
Within
CF
lungs,
P.
persists
conducting
airways
together
human
mucins
as
most
abundant
structural
component
of
its
microenvironment.
We
investigated
adhesion
41
serial
isolates
to
mucin
preparations
from
sputa.
Mucins
and
bacteria
were
retrieved
five
modulator-naïve
patients
advanced
lung
disease.
non-CF
reference
strains
showed
a
strain-specific
signature
their
ovine,
porcine
bovine
submaxillary
ranging
no
or
low
moderate
strong
binding.
Serial
clonal
colony
morphotypes
same
sputum
sample
heterogeneous
affinity
representatives
other
clones
thus
making
‘mucin
binding’
one
variable
intraclonal
phenotypic
traits
known
date.
Most
did
not
adhere
more
strongly
than
plastic
surfaces.
binders,
however,
exhibited
gradient
O
-glycans,
mammalian
mucins.
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Май 15, 2025
Abstract
Background
Mycobacterium
avium
complex
causes
chronic
and
difficult-to-treat
infection
in
vulnerable
patient
groups,
incidence
is
increasing
worldwide.
Whole
genome
sequencing
has
the
potential
to
reveal
new
information
about
how
M.
persists
over
time
human
lung.
Methods
We
analysed
genomes
of
287
isolates
that
were
sampled
longitudinally
from
56
patients.
Our
dataset
included
50
newly
sequenced
a
cohort
20
patients
Ireland
who
for
up
10
years,
we
compared
these
237
published
2
pre-existing
cohorts
Europe
evaluate
strains
wider
context.
Additionally,
performed
combined
analysis
across
3
examine
changes
occurred
course
infection.
Results
identified
6
instances
where
clustered
with
within
12-SNP
threshold,
supporting
previous
observations
dominant
circulating
clones
are
present
internationally.
Across
cohorts,
found
communities
evolved
individual
hosts,
report
acquisition
frequent.
Importantly,
our
findings
suggest
may
adapt
conditions
it
faces
host,
evidence
positive
selection
12
distinct
mycobacterial
genes.
Notably,
multiple
virulence-associated
genes
under
selection,
including
could
confer
resistance
antibiotics
host
defence
mechanisms.
Conclusion
provides
novel
insights
into
within-host
evolution
highlights
potentially
important
strategies
enhance
persistence
provide
targets
therapeutic
investigation.
In
this
opinion
piece,
we
consider
the
meaning
of
term
‘wild
type’
in
context
microbiology.
This
is
especially
pertinent
post-genomic
era,
where
have
a
greater
awareness
species
diversity
than
ever
before.
Genomic
heterogeneity,
vitro
evolution/selection
pressures,
definition
‘the
wild’,
size
and
importance
pan-genome,
gene–gene
interactions
(epistasis),
nature
‘wild-type
gene’
are
all
discussed.
We
conclude
that
wild
type
an
outdated
even
misleading
phrase
should
be
gradually
phased
out.
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2024,
Номер
14
Опубликована: Авг. 22, 2024
The
chronic
airway
infections
with
Pseudomonas
aeruginosa
are
the
major
co-morbidity
in
people
cystic
fibrosis
(CF).
Within
CF
lungs,
P.
persists
conducting
airways
together
human
mucins
as
most
abundant
structural
component
of
its
microenvironment.
We
investigated
adhesion
41
serial
isolates
to
mucin
preparations
from
sputa.
Mucins
and
bacteria
were
retrieved
five
modulator-naïve
patients
advanced
lung
disease.
non-CF
reference
strains
showed
a
strain-specific
signature
their
ovine,
porcine
bovine
submaxillary
ranging
no
or
low
moderate
strong
binding.
Serial
clonal
colony
morphotypes
same
sputum
sample
heterogeneous
affinity
representatives
other
clones
thus
making
‘mucin
binding’
one
variable
intraclonal
phenotypic
traits
known
date.
Most
did
not
adhere
more
strongly
than
plastic
surfaces.
binders,
however,
exhibited
gradient
O
-glycans,
mammalian
mucins.
