Biomedicines,
Год журнала:
2025,
Номер
13(2), С. 446 - 446
Опубликована: Фев. 12, 2025
Background:
Microplastics
(MPs)
are
small
plastic
fragments
with
diameters
less
than
5
mm
in
size
and
prevalent
everyday
essentials
consumables.
Large
global
production
has
now
led
to
a
flooding
of
MPs
our
natural
environment.
Due
their
detrimental
impacts
on
the
planet's
ecosystems
potentially
health,
have
emerged
as
significant
public
health
concern.
In
this
pilot
study,
we
hypothesize
that
exposure
will
negatively
affect
gut
microbiota
composition
function,
which
metabolic
reprogramming
plays
an
important
role.
Methods:
Using
vitro
experiments,
three
bacterial
strains
(Escherichia
coli
MG1655,
Nissle
1917,
Lactobacillus
rhamnosus)
were
selected
investigate
exposure.
The
individually
cultured
anaerobic
chamber
exposed
1
µm
polystyrene
at
various
concentrations
(0,
10,
20,
50,
100,
500
µg/mL)
culture
medium.
Results:
reduced
growth
all
dose-dependent
manner.
Liquid
chromatography
mass
spectrometry
(LC-MS)-based
untargeted
metabolomics
revealed
differences
multiple
pathways,
such
sulfur
metabolism
amino
sugar
nucleotide
metabolism.
addition,
extracted
from
C57BL/6
mice,
16S
rRNA
sequencing
results
showed
upregulation
Lactobacillales
reduction
Erysipelotrichales
due
Furthermore,
targeted
corroborated
alterations
microbial
tryptophan
energy
producing
glycolysis/gluconeogenesis
pentose
phosphate
pathway.
Conclusions:
These
findings
provide
evidence
causes
comprehensive
changes
healthy
microbiota,
may
also
insights
into
mechanistic
effects
humans.
Physiologia Plantarum,
Год журнала:
2025,
Номер
177(1)
Опубликована: Янв. 1, 2025
Abstract
Plastic
film
(mulch
film)
is
widely
used
in
saline
and
alkaline
soils
because
it
can
effectively
reduce
salt
stress
damage.
However,
results
the
accumulation
of
microplastics
(MPs)
soil,
which
pose
a
threat
to
crop
growth
production.
This
study
investigates
effects
50
mg
l
−1
MPs
100
mM
sodium
chloride
(NaCl),
individually
or
combination,
on
physiological
characteristics
maize
(
Zea
mays
)
seedlings.
The
demonstrated
that
compared
control,
NaCl
single
combined
reduced
seedling
biomass
water
content,
was
more
serious.
Stress
significantly
N
K
contents
leaves,
Na
content
under
lower
than
stress.
Compared
stress,
further
enhanced
oxidative
damage
by
increasing
H
2
O
MDA
disrupted
chloroplast
structure,
chlorophyll
ultimately
leading
decline
fluorescence
parameters
photosynthetic
efficiency.
Single
led
proline,
soluble
proteins,
sugars,
while
stresses
increased
these
osmotic
substances
plants.
Moreover,
activity
CAT,
POD,
SOD
AsA
GsH.
Collectively,
exert
notable
toxic
growth.
Although
inhibited
showed
antagonistic
effects.
These
findings
underscore
importance
assessing
ecological
risks
posed
Biomedicines,
Год журнала:
2025,
Номер
13(2), С. 446 - 446
Опубликована: Фев. 12, 2025
Background:
Microplastics
(MPs)
are
small
plastic
fragments
with
diameters
less
than
5
mm
in
size
and
prevalent
everyday
essentials
consumables.
Large
global
production
has
now
led
to
a
flooding
of
MPs
our
natural
environment.
Due
their
detrimental
impacts
on
the
planet's
ecosystems
potentially
health,
have
emerged
as
significant
public
health
concern.
In
this
pilot
study,
we
hypothesize
that
exposure
will
negatively
affect
gut
microbiota
composition
function,
which
metabolic
reprogramming
plays
an
important
role.
Methods:
Using
vitro
experiments,
three
bacterial
strains
(Escherichia
coli
MG1655,
Nissle
1917,
Lactobacillus
rhamnosus)
were
selected
investigate
exposure.
The
individually
cultured
anaerobic
chamber
exposed
1
µm
polystyrene
at
various
concentrations
(0,
10,
20,
50,
100,
500
µg/mL)
culture
medium.
Results:
reduced
growth
all
dose-dependent
manner.
Liquid
chromatography
mass
spectrometry
(LC-MS)-based
untargeted
metabolomics
revealed
differences
multiple
pathways,
such
sulfur
metabolism
amino
sugar
nucleotide
metabolism.
addition,
extracted
from
C57BL/6
mice,
16S
rRNA
sequencing
results
showed
upregulation
Lactobacillales
reduction
Erysipelotrichales
due
Furthermore,
targeted
corroborated
alterations
microbial
tryptophan
energy
producing
glycolysis/gluconeogenesis
pentose
phosphate
pathway.
Conclusions:
These
findings
provide
evidence
causes
comprehensive
changes
healthy
microbiota,
may
also
insights
into
mechanistic
effects
humans.