A naturally isolated symbiotic bacterium suppresses flavivirus transmission by Aedes mosquitoes

Science, Год журнала: 2024, Номер 384(6693)

Опубликована: Апрель 18, 2024

The commensal microbiota of the mosquito gut plays a complex role in determining vector competence for arboviruses. In this study, we identified bacterium from field Aedes albopictus mosquitoes named Rosenbergiella sp. YN46 ( Rosenbergiella_ YN46) that rendered refractory to infection with dengue and Zika viruses. Inoculation 1.6 × 10 3 colony forming units (CFUs) into A. effectively prevents viral infection. Mechanistically, secretes glucose dehydrogenase Ry GDH), which acidifies lumen fed mosquitoes, causing irreversible conformational changes flavivirus envelope protein prevent entry cells. semifield conditions, exhibits effective transstadial transmission blocks virus by newly emerged adult mosquitoes. prevalence is greater low-dengue areas (52.9 ~91.7%) than those dengue-endemic regions (0 ~6.7%). may offer an safe lead biocontrol.

Язык: Английский

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Mosquito host background influences microbiome-ZIKV interactions in field and laboratory-reared Aedes aegypti

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 2, 2025

Abstract The mosquito microbiota represents an intricate assemblage of microorganisms, comprising bacteria, fungi, viruses, and protozoa. Factors modulating microbiome abundance composition include host genetic background, environmental parameters, pathogen exposure. Conversely, the profoundly influences infection thus harbours considerable potential to impact transmission vector-borne diseases. As such, there is a growing interest in using novel vector-control strategies, including exploiting natural ability some microbes interfere with vectors by pathogens. However, before microbiome-based vector control approaches can move towards translation, more complete understanding interactions between mosquitoes, their microbiome, pathogens they transmit, required better appreciate how variation field mosquitoes affects these interactions. To examine background associated diversity microbiomes within distinct hosts, but without artificially manipulating we exposed several laboratory-reared field-collected Aedes aegypti lines Zika virus (ZIKV) correlated microbial load exposure viral success. We observed significant differences ZIKV outcomes different microbiomes, found that alteration was lines. also identified taxa correlating either or lack infection. In summary, our study provides insights into variability holobiont. A which factors influence tripartite arboviral pathogens, will be critical for development microbial-based interventions aimed at reducing disease burden. Author summary. differs individual across its development, as well same developmental stage, spatially genomically populations. highly relevant transmit Furthermore, certain have been shown mosquito, while conversely, alter microbiome. poor universally conserved pathogen-related effects specific host-microbiome combination are populations respective microbiomes. address this, infected lines, reared laboratory caught examined after compared unchallenged progressed correlations further changes. responses differed potentially due hosts. Alternatively, may respond differently infection, subsequently alters manner, occur. being evaluated mosquito-borne disease, findings contribute host-microbe develop approaches. Variation need considered experimental designs when interpreting results from studies. It especially deployment where known higher

Язык: Английский

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Harnessing engineered symbionts to combat concurrent malaria and arboviruses transmission

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 1, 2025

Concurrent malaria and arbovirus infections pose significant public health challenges in tropical subtropical regions, demanding innovative control strategies. Here, we describe a strategy that employs multifunctional engineered symbiotic bacteria to suppress concurrent transmission of parasites, dengue, Zika viruses by various vector mosquitoes. The bacterium Serratia AS1, which efficiently spreads through Anopheles Aedes populations, is simultaneously produce anti-Plasmodium anti-arbovirus effector proteins controlled selected blood-induced promoter. Laboratory outdoor field-cage studies show the strains effectively inhibit Plasmodium infection mosquitoes Our findings provide foundation for use as powerful tool combat diseases. Engineered with secretion multiple effectors (Dengue Zika) mosquitoes, providing promising these

Язык: Английский

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Exploring the Diversity of Microbial Communities Associated with Two Anopheles Species During Dry Season in an Indigenous Community from the Colombian Amazon

Insects, Год журнала: 2025, Номер 16(3), С. 269 - 269

Опубликована: Март 4, 2025

Malaria disease affects millions of people annually, making the Amazon Basin a major hotspot in Americas. While traditional control strategies rely on physical and chemical methods, Anopheles microbiome offers promising avenue for biological control, as certain bacteria can inhibit parasite development alter vector immune reproductive systems, disrupting transmission cycle. For this reason, study aimed to explore bacterial communities An. darlingi triannulatus s.l., including breeding sites, immature stages, adults from San Pedro de los Lagos (Leticia, Amazonas) through next-generation sequencing 16S rRNA gene. The results revealed higher genus richness L1–L2 larvae s.l. Aeromonas Enterobacter were prevalent most samples, with abundances 52.51% L3–L4 48.88% pupae respectively. In site water, Verrucomicrobiota dominant (52.39%). We also identified Delftia (15.46%) Asaia (98.22%) triannulatus, linked Plasmodium inhibition, Elizabethkingia, low abundances, along Klebsiella Serratia, known paratransgenesis potential. Considering high diversity observed across different mosquito life identifying composition is first step towards developing new malaria control. However, specific roles these anophelines cycle remain be elucidated.

Язык: Английский

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Current insights into human pathogenic phenuiviruses and the host immune system

Virulence, Год журнала: 2024, Номер 15(1)

Опубликована: Июль 29, 2024

Phenuiviruses are a class of segmented negative-sense single-stranded RNA viruses, typically consisting three segments that encode four distinct proteins. The emergence pathogenic phenuivirus strains, such as Rift Valley fever phlebovirus (RVFV) in sub-Saharan Africa, Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) East and Southeast Asia, Heartland (HRTV) the United States has presented considerable challenges to global public health recent years. innate immune system plays crucial role initial defense mechanism host against invading pathogens. In addition continued research aimed at elucidating epidemiological characteristics phenuivirus, significant advancements have been made investigating its viral virulence factors (glycoprotein, non-structural protein, nucleoprotein) potential host–pathogen interactions. Specifically, efforts focused on understanding mechanisms evasion, assembly egress, networks involving cells, programmed cell death, inflammation, nucleic acid receptors, etc. Furthermore, plethora technological advancements, including metagenomics, metabolomics, single-cell transcriptomics, proteomics, gene editing, monoclonal antibodies, vaccines, utilized further our pathogenesis responses. Hence, this review aims provide comprehensive overview current recognition, therapeutic approaches during human infections focusing particularly RVFV SFTSV.

Язык: Английский

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Metatranscriptomic insights into the dengue patient blood microbiome: Enhanced microbial diversity and metabolic activity in severe patients

PLoS neglected tropical diseases, Год журнала: 2024, Номер 18(10), С. e0012589 - e0012589

Опубликована: Окт. 17, 2024

Background Dengue is the most re-emergent infection, with approximately 100 million new cases reported annually, yet no effective treatment or vaccine exists. Here, we aim to define microbial community structure and their functional profiles in dengue positive patients varying disease severity. Methodology/Principal findings Hospital admitted 112 dengue-positive blood samples were analyzed by dual RNA-sequencing simultaneously identify transcriptionally active microbes (TAMs), expressed genes associated pathways. Results highlight that severe exhibited increased diversity presence of opportunistic species (unique core) which includes Bacillus cereus , Burkholderia pseudomallei Streptococcus suis Serratia marcescens . The profile analysis revealed enriched metabolic pathways such as protein degradation, nucleotide biosynthesis, ion transport, cell shape integrity, ATP formation cases, indicating high energy demands adaptability these microbes. Conclusion Our metatranscriptomic approach provides a species-level characterization microbiome composition reveals heightened TAMs dengue, underscoring need for further research into role microbiota progression. Comparing signatures across severity classes early offers unique potential convenient diagnosis infection.

Язык: Английский

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The Japanese encephalitis virus NS1′ protein facilitates virus infection in mosquitoes

PLoS neglected tropical diseases, Год журнала: 2025, Номер 19(1), С. e0012823 - e0012823

Опубликована: Янв. 27, 2025

Background The Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, is known for its capacity to cause severe neurological disease in Asia. Neurotropic flaviviruses within the (JE) serogroup possess distinctive feature of expressing unique nonstructural protein, NS1′. NS1′ protein consists full NS1 with an additional 52 amino acid extension at C-terminus and has been demonstrated exhibit virulence mammalian hosts upon infection. However, precise role mosquito vectors yet be elucidated. Methodology/principal findings In this study, NS1′-defective (rG66A) was engineered, effect on infection cells investigated. results significant reduction infectivity rG66A by RT-qPCR, indicating that absence impedes JEV replication Culex mosquitoes. Additionally, research elucidated underlying mechanism which enhances viral mosquitoes RNA-Seq analysis. Specifically, found facilitate through suppression antimicrobial peptides (AMPs) regulated Toll pathway. Conclusions/significance Our contributes immune escape, thereby enhancing This finding offers new insights into transmission mechanisms JEV, elucidating novel aspects propagation.

Язык: Английский

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First-ever ASM Global Research Symposium on Microbes in Human Health in Tsinghua, Beijing, China

mSphere, Год журнала: 2025, Номер unknown

Опубликована: Фев. 24, 2025

ABSTRACT The American Society for Microbiology Global Research Symposium on Microbes in Human Health, hosted partnership with Tsinghua University, was held Beijing, China, 25 to 27 September 2024. conference provided an international forum microbiologists from different disciplines present and discuss new findings. meeting covered a wide range of topics, spanning across bacteria, virus, fungi, hosts. This report summarizes the presentations emerging themes.

Язык: Английский

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RAB4B and Japanese encephalitis virus E protein interaction is essential for viral entry in early endosomes

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 306, С. 141452 - 141452

Опубликована: Фев. 26, 2025

Язык: Английский

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Comparison of innate immune responses against arboviruses in mammalian hosts and mosquito vectors

hLife, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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