Multiplex generation and single-cell analysis of structural variants in mammalian genomes

Science, Год журнала: 2025, Номер 387(6733)

Опубликована: Янв. 30, 2025

Studying the functional consequences of structural variants (SVs) in mammalian genomes is challenging because (i) SVs arise much less commonly than single-nucleotide or small indels and (ii) methods to generate, map, characterize model systems are underdeveloped. To address these challenges, we developed Genome-Shuffle-seq, a method that enables multiplex generation mapping thousands (deletions, inversions, translocations, extrachromosomal circles) throughout genomes. We also demonstrate co-capture SV identity with single-cell transcriptomes, facilitating measurement impact on gene expression. anticipate Genome-Shuffle-seq will be broadly useful for systematic exploration expression, chromatin landscape, three-dimensional nuclear architecture, while initiating path toward minimal genome.

Язык: Английский

Genome recombination on demand

Science, Год журнала: 2025, Номер 387(6733), С. 477 - 478

Опубликована: Янв. 30, 2025

Large genome rearrangements in mammalian cells can be generated at scale

Язык: Английский

Selective targeting of genome amplifications and repeat elements by CRISPR-Cas9 nickases to promote cancer cell death

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Июнь 2, 2025

Язык: Английский