Evolutionary Constraints on RNA Polymerase Gene Positioning in the Genome of Fast-Growing Bacteria DOI Creative Commons
Leticia Larotonda,

E. Ojeda,

Matteo Bordignon

и другие.

Опубликована: Апрель 14, 2025

Abstract How gene order along chromosomes affects cellular homeostasis and genome evolution remains poorly understood. Bacterial are organized the replication origin ( oriC )–terminus ter ) axis. The spatial arrengement of genes within this axis may influence physiology, evolution, transcriptional regulation. catalytic core sole bacterial RNA polymerase (RNAP) is encoded by rpoB rpoC universally conserved rplKAJL-rpoBC locus. In fast growing bacteria locus located near potentially linking organization to physiology. Here, we investigated functional relevance chromosomal positioning relocating it Vibrio cholerae genome. While viable, strains harboring far from exhibited nutrient-dependent fitness defects. These phenotypes correlated with reduced copy number RNAP abundance, without changes in its subcellular distribution. Introducing a second rpIKAJL-rpoBC at distal genomic location abolished phenotypes, demonstrating that replication-associated dosage effects were main mechanism behind observed physiological changes. Further uncoupling revealed costs specifically linked relocation. Our results reveal selective pressures act maintain RNAP-encoding optimize effects, ensuring efficient production during exponential growth. We discuss relationship between ecological strategies work underscores as an underestimated but critical factor shaping physiology evolution. Significance have highly plastic content importance their started be appreciated driver Growing evidence suggests genomes plays role expression, dynamics, fitness. study, demonstrate , encoding (RNAP), essential for optimal By show position directly impacts fitness, effects. findings suggest ensure output More broadly, our study highlights underappreciated beyond known roles operon structure Given fundamental RNAP, implications understanding strategies, adaptation, synthetic design. integrating positional genomics studies, provide theoretical experimental framework investigating how architecture shapes function.

Язык: Английский

Living on the edge: Mucus-associated microbes in the colon DOI Creative Commons
Mihovil Joja, Erica T. Grant, Mahesh S. Desai

и другие.

Mucosal Immunology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

The colonic mucus layer acts as a physicochemical barrier to pathogen invasion and habitat for mucus-associated microbes. This mucosal microbiome plays crucial role in moderating production, maintaining integrity, shaping the host immune response. However, unchecked mucin foraging may render vulnerable disease. To better understand these dynamics layer, it is essential advance fundamental knowledge on how commensals bind utilize well their interactions with both microbial neighbors. We present an overview of approaches surveying bacteria assessing mucin-utilizing capacity, alongside discussion limitations existing methods. Additionally, we highlight diet secretory immunoglobulin A interact bacterial community colon. Insights into this subset can guide therapeutic strategies optimally support modulate integrity.

Язык: Английский

Процитировано

0
Tolerance and heteroresistance to echinocandins in Candida auris : conceptual issues, clinical implications, and outstanding questions DOI Creative Commons
Erika Shor, David S. Perlin, Dimitrios P. Kontoyiannis

и другие.

mSphere, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

ABSTRACT Candida auris is a significant public health threat due to its environmental persistence and multidrug resistance, with echinocandins being the preferred treatment. However, in addition echinocandin tolerance heteroresistance may contribute treatment challenges. Echinocandin involves reduced drug-mediated killing, while ability of small cell subset grow at high drug concentrations. These phenomena facilitate emergence full resistance complicate clinical outcomes. The significance these mechanisms remains unclear, limited data correlating them failures. Research needed understand their impact, develop streamlined robust methods detect settings, explore mitigation strategies. pathogen’s range adaptations demands innovative approaches like spatial transcriptomics dissect complex responses improve patient

Язык: Английский

Процитировано

0
Evolutionary Constraints on RNA Polymerase Gene Positioning in the Genome of Fast-Growing Bacteria DOI Creative Commons
Leticia Larotonda,

E. Ojeda,

Matteo Bordignon

и другие.

Опубликована: Апрель 14, 2025

Abstract How gene order along chromosomes affects cellular homeostasis and genome evolution remains poorly understood. Bacterial are organized the replication origin ( oriC )–terminus ter ) axis. The spatial arrengement of genes within this axis may influence physiology, evolution, transcriptional regulation. catalytic core sole bacterial RNA polymerase (RNAP) is encoded by rpoB rpoC universally conserved rplKAJL-rpoBC locus. In fast growing bacteria locus located near potentially linking organization to physiology. Here, we investigated functional relevance chromosomal positioning relocating it Vibrio cholerae genome. While viable, strains harboring far from exhibited nutrient-dependent fitness defects. These phenotypes correlated with reduced copy number RNAP abundance, without changes in its subcellular distribution. Introducing a second rpIKAJL-rpoBC at distal genomic location abolished phenotypes, demonstrating that replication-associated dosage effects were main mechanism behind observed physiological changes. Further uncoupling revealed costs specifically linked relocation. Our results reveal selective pressures act maintain RNAP-encoding optimize effects, ensuring efficient production during exponential growth. We discuss relationship between ecological strategies work underscores as an underestimated but critical factor shaping physiology evolution. Significance have highly plastic content importance their started be appreciated driver Growing evidence suggests genomes plays role expression, dynamics, fitness. study, demonstrate , encoding (RNAP), essential for optimal By show position directly impacts fitness, effects. findings suggest ensure output More broadly, our study highlights underappreciated beyond known roles operon structure Given fundamental RNAP, implications understanding strategies, adaptation, synthetic design. integrating positional genomics studies, provide theoretical experimental framework investigating how architecture shapes function.

Язык: Английский

Процитировано

0