TIR domains produce histidine-ADPR as an immune signal in bacteria
Nature,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 30, 2025
Язык: Английский
Self-sacrificing cell death to thwart phage infection
Nature Reviews Microbiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 13, 2025
Язык: Английский
Chemical inhibition of a bacterial immune system
Zhiyu Zang,
Olivia K. Duncan,
Dziugas Sabonis
и другие.
Опубликована: Фев. 21, 2025
Abstract
The
rise
of
antibiotic
resistance
motivates
a
revived
interest
in
phage
therapy.
However,
bacteria
possess
dozens
anti-bacteriophage
immune
systems
that
confer
to
therapeutic
phages.
Chemical
inhibitors
these
anti-phage
could
be
employed
as
adjuvants
overcome
phage-based
therapies.
Here,
we
report
can
selectively
inhibited
by
small
molecules,
thereby
sensitizing
phage-resistant
We
discovered
class
chemical
inhibit
the
type
II
Thoeris
system.
These
block
biosynthesis
histidine-ADPR
intracellular
‘alarm’
signal
ThsB
and
prevent
ThsA
from
arresting
replication.
promiscuously
diverse
bacteria—including
antibiotic-resistant
pathogens.
inhibition
defense
improved
efficacy
model
therapy
against
strain
P.
aeruginosa
mouse
infection,
suggesting
potential.
Furthermore,
may
tools
dissect
importance
system
for
natural
microbial
communities.
Язык: Английский
Proteome-wide discovery of phage anti-defense proteins by Alphafold2
Molecular Cell,
Год журнала:
2025,
Номер
85(6), С. 1052 - 1053
Опубликована: Март 1, 2025
Язык: Английский
Cat1 forms filament networks to degrade NAD + during the type III CRISPR-Cas antiviral response
Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 10, 2025
Type
III
CRISPR-Cas
systems
defend
against
viral
infection
in
prokaryotes
using
an
RNA-guided
complex
that
recognizes
foreign
transcripts
and
synthesizes
cyclic
oligo-adenylate
(cOA)
messengers
to
activate
CARF
immune
effectors.
Here
we
investigated
a
protein
containing
domain
fused
Toll/interleukin-1
receptor
(TIR)
domain,
Cat1.
We
found
Cat1
provides
immunity
by
cleaving
depleting
NAD
+
molecules
from
the
infected
host,
inducing
growth
arrest
prevents
propagation.
forms
dimers
stack
upon
each
other
generate
long
filaments
are
maintained
bound
cOA
ligands,
with
stacked
TIR
domains
forming
cleavage
catalytic
sites.
Further,
assemble
into
unique
trigonal
pentagonal
networks
enhance
degradation.
presents
unprecedented
chemistry
higher-order
assembly
for
response.
Язык: Английский
Recognition-Dependent Activation of the Rrs1/Rps4 Immune Receptor Complex
Опубликована: Янв. 1, 2025
Язык: Английский
Natural pentafuranosylnucleos(t)ides and their analogs: structure, functions, synthesis, and perspective of medical application
Опубликована: Май 29, 2025
Pentafuranosylnucleos(t)ides
represent
a
class
of
natural
compounds
regulating
diverse
cell
functions
being
preferably
components
biopolymers
and
also
participating
as
cyclic
regulatory
low-molecular
ligands.
Disaccharide
nucleosides
related
analogs
are
considered
therapeutically
potent
for
the
treatment
cancer,
viral
diseases,
variety
metabolic
disorders
by
mimicking
structure
biochemically
occurring
molecules
in
nicotinamide
adenine
dinucleotide
(NAD+)
transformation.
Several
approaches
have
been
developed
on
way
to
chemical
synthesis
poly(adenosine
diphosphate
ribose)
(PAR),
unique
biopolymer
taking
part
DNA
repair
associated
functions,
that
would
allow
extensive
studies
molecular
mechanisms
diseases.
The
present
review
consists
following
main
parts,
first
one
including
structural
characterization,
biochemical
roles,
disaccharide
from
different
sources
their
basis,
second
describing
therapeutic
applications
analogs.
General
conclusion
perspectives
summarized
last
part.
Язык: Английский