Stress-induced organismal death is genetically regulated by the mTOR-Zeste-Phae1 axis DOI Creative Commons
Takashi Matsumura, Masasuke Ryuda, Hitoshi Matsumoto

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 25, 2024

Abstract All organisms are exposed to various stressors, which can sometimes lead organismal death, depending on their intensity. While stress-induced death has been observed in many species, the underlying mechanisms remain unclear. In this study, we investigated molecular of fruit fly Drosophila melanogaster . We identified a chymotrypsin-like serine protease Phaedra1 ( Phae1 ) as mediator D. larvae. expression was upregulated by lethal heat stress (40 °C) but not non-lethal (38 °C or lower). The most prominent induction occurred central nervous system (CNS). found neuro-specific knockdown increased survival and reduced neuronal caspase activity following exposure stress, suggesting that transcriptional upregulation CNS is essential for death. next via bioinformatic biochemical analyses transcription factor Zeste (Z) bound enhancer region z loss-of-function impaired CNS, increasing stress. addition, chemical screening rapamycin, inhibitor mechanistic target rapamycin (mTOR), suppressed expression. Neuro-specific mTor protein levels both Z, leading an increase Together, these results indicate larvae regulated genetically encoded signaling pathway involving mTOR-Z-Phae1 axis.

Язык: Английский

Stellate Ganglion Block reduces symptoms of SARS-CoV-2-induced ME/CFS: A prospective cohort pilot study DOI Creative Commons
Deborah Duricka,

Luke D. Liu

Fatigue Biomedicine Health & Behavior, Год журнала: 2025, Номер unknown, С. 1 - 18

Опубликована: Фев. 6, 2025

Background Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a debilitating condition characterized by fatigue, orthostatic intolerance (OI), post-exertional malaise (PEM) and unrefreshing sleep. Our previous work has shown that modulating the autonomic nervous system can alleviate symptoms of Long COVID, which shares striking similarities with ME/CFS.

Язык: Английский

Процитировано

0
Hypoxic compound exercise improves cardiac function in Drosophila high fructose diet via KHK DOI
Ping Xu, Qiufang Li, Meng Ding

и другие.

Journal of Molecular and Cellular Cardiology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Transcriptome analysis reveals that the injection of mesenchymal stem cells remodels extracellular matrix and complement components of the brain through PI3K/AKT/FOXO1 signaling pathway in a neuroinflammation mouse model DOI Creative Commons

Zhihao Xu,

Keqin Liu,

Guoqing Zhang

и другие.

Genomics, Год журнала: 2025, Номер 117(3), С. 111033 - 111033

Опубликована: Март 22, 2025

Язык: Английский

Процитировано

0
Preoperative quantitative quadriceps muscle ultrasound to predict POD for gastrointestinal surgery in older patients DOI Creative Commons

Cunjin Wang,

Xiaowei Song, Lan Cao

и другие.

BMC Gastroenterology, Год журнала: 2025, Номер 25(1)

Опубликована: Март 24, 2025

Postoperative delirium (POD) is generally associated with increased postoperative adverse events. We aimed to investigate whether preoperative quantitative quadriceps muscle ultrasound could predict POD in older patients after gastrointestinal surgery order provide more targeted prevention and treatment measures. prospectively collected data from elderly who underwent elective August December 2023 at a tertiary hospital China. Intergroup difference analysis univariate multivariate logistic regression analyses were used explore independent risk factors. calculated assessed the parameters via sensitivity, specificity, Youden index, area under receiver operating characteristic curve (AUC), calibration curves Hosmer-Lemeshow test. The nomogram was validated internally through bootstrap resampling. decision (DCA) evaluate its clinical validity. A total of 695 analyzed this investigation, among which 137 experienced an incidence rate 19.7%. After conducting using R software, six factors identified, including age, thickness (Q-MT), echo intensity (Q-EI), Charlson Comorbidity Index (CCI), frailty Minimum Mental State Examination (MMSE) scores. AUC value model 0.966 (95% CI: 0.950–0.982; p < 0.05). revealed that predicted probability consistent actual probability, Hosmer–Lemeshow goodness-of-fit test 0.811. DCA has net benefit for POD. Quantitative parameters, Q-MT Q-EI, patients. have developed new predicting undergo surgery. trial registered Chinese Clinical Trial Registry ( http://www.chictr.org.cn/ ) on 3, 2023, registration number ChiCTR2300074304.

Язык: Английский

Процитировано

0
Astrocytes-derived LCN2 triggers EV-A71–induced muscle soreness via accumulating lactate DOI Creative Commons
Qiao You, Jing Wu,

Chaoyong Wang

и другие.

Science Advances, Год журнала: 2025, Номер 11(20)

Опубликована: Май 16, 2025

Viral muscle soreness (VMS) is a common feature during acute viral infections, including those caused by enteroviruses, and it substantially diminishes patients’ quality of life. At present, we aim to establish the “brain-muscle” axis explore underlying mechanisms VMS. We initially observed that diminished pain threshold occurred in enterovirus A71 (EV-A71)–infected C57BL/6J AG6 mice. Subsequently, RNA sequencing data showed lipocalin 2 (LCN2) was up-regulated multiple EV-A71, Japanese encephalitis virus, vesicular stomatitis West Nile which all As expected, Lcn2 -deficient C57BL/6 J ( −/− ) mice exhibited greater tolerance, as shown stronger grip force stable motor function after EV-A71 infection. Mechanistically, EV-A71–induced high-mobility group 1 (HMGB1) stimulated astrocyte-derived LCN2 secreted into circulatory system, enhanced glycolysis induced lactate buildup through increasing pyruvate dehydrogenase kinase (PDK1) expression decreasing (PDH) activity. Together, HMGB1/LCN2/PDK1/lactate pathway brain-muscle promoted VMS development.

Язык: Английский

Процитировано

0
Core features and inherent diversity of post-acute infection syndromes DOI Creative Commons
Alain Trautmann

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Июнь 3, 2025

Post-acute infection syndromes (PAIS), i.e., long-lasting pathologies subsequent to infections that do not properly resolve, have both a common core and broad diversity of manifestations. PAIS include group symptoms (pathological fatigue, cognitive problems, sleep disorders pain) accompanied by large set diverse symptoms. Core additional symptoms, which can persist for years, exhibiting periods relapses remissions, usually start suddenly after an apparently infection. display highly variable clinical features depending on the nature initial pathogen, even larger extent, preexisting individual terrains in are rooted. In first part, I discuss biological issues related persistence microbial antigens, dysregulated immune responses, reactivation latent viruses, different potential self-sustained inflammatory loops, mitochondrial dysfunction, metabolic tryptophan- kynurenin pathway (TKP) with impact serotonin, consequences dysfunctional bidirectional microbiota-gut-brain axis. The second part deals nervous system dependence PAIS. rely concept interoception, process brain senses, integrates interprets signals originating from within body, sends feebacks aimed at maintaining homeostasis. Interoception is central understanding origin dysautonomia, dysfunctioning hypothalamus-pituitary-adrenal (HPA) axis, its relation stress, inflammation or depression. propose all predispositions leading vicious circles constitute building blocks self-assemble many possible ways, give rise A useful discrimination between subtypes should be obtained composite profiling including biomarkers, questionnaires functional tests so as take into account multidimensionality.

Язык: Английский

Процитировано

0
Transcriptomic profiling of “brain-eating amoeba” Naegleria fowleri infection in mice: the host and the protozoa perspectives DOI Creative Commons

Vincent Guerlais,

Nina Allouch,

E. Ashley Moseman

и другие.

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Дек. 15, 2024

The free-living amoeba

Язык: Английский

Процитировано

2
How neuroinflammation weakens muscles DOI

Alexandra Flemming

Nature reviews. Immunology, Год журнала: 2024, Номер 24(9), С. 619 - 619

Опубликована: Июль 29, 2024

Язык: Английский

Процитировано

0
Stress-induced organismal death is genetically regulated by the mTOR-Zeste-Phae1 axis DOI Creative Commons
Takashi Matsumura, Masasuke Ryuda, Hitoshi Matsumoto

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 25, 2024

Abstract All organisms are exposed to various stressors, which can sometimes lead organismal death, depending on their intensity. While stress-induced death has been observed in many species, the underlying mechanisms remain unclear. In this study, we investigated molecular of fruit fly Drosophila melanogaster . We identified a chymotrypsin-like serine protease Phaedra1 ( Phae1 ) as mediator D. larvae. expression was upregulated by lethal heat stress (40 °C) but not non-lethal (38 °C or lower). The most prominent induction occurred central nervous system (CNS). found neuro-specific knockdown increased survival and reduced neuronal caspase activity following exposure stress, suggesting that transcriptional upregulation CNS is essential for death. next via bioinformatic biochemical analyses transcription factor Zeste (Z) bound enhancer region z loss-of-function impaired CNS, increasing stress. addition, chemical screening rapamycin, inhibitor mechanistic target rapamycin (mTOR), suppressed expression. Neuro-specific mTor protein levels both Z, leading an increase Together, these results indicate larvae regulated genetically encoded signaling pathway involving mTOR-Z-Phae1 axis.

Язык: Английский

Процитировано

0