Dynamic protein phosphorylation inStreptococcus pyogenesduring growth, stationary phase and starvation DOI Creative Commons
Stefan Mikkat, Michael Kreutzer, Nadja Patenge

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 25, 2023

Abstract Background Phosphorylation of proteins at serine, threonine, and tyrosine residues plays an important role in physiological processes bacteria such as cell cycle, metabolism, virulence, dormancy, sporulation, stationary phase functions. Streptococcus pyogenes possesses a single known transmembrane serine/threonine kinase belonging to the class penicillin-binding protein serine/threonine-associated (PASTA) kinases. To gain initial insights into targets dynamics phosphorylation S. serotype M49 strain 591, we performed proteomics phosphoproteomics workflow using cultures from different growth conditions, starvation. Results The quantitative analysis dynamic phosphorylation, which included subset 463 out 815 identified sites, revealed two main types events, distinguished by they predominantly occur their preference for either threonine or serine. A small group events occurred almost exclusively related cycle was enhanced growing cells. Many these sites are highly conserved PASTA kinases streptococci. majority during starvation, preferentially serine residues. These phosphorylations may be regulatory persister formation, but function responsible formation need elucidated further analyses. Moreover, our data indicate that vast can phosphorylated, identification phosphopeptides depends on sensitivity proteomic methods used. Conclusions kinase-dependent regulation found , account only part all events. Most take place This phenomenon has also been described some other therefore general feature bacterial phosphorylation.

Язык: Английский

Progress in the Prevalence, Classification and Drug Resistance Mechanisms of Methicillin-Resistant Staphylococcus aureus DOI Creative Commons
Zhuru Hou, Ling Liu,

Jianhong Wei

и другие.

Infection and Drug Resistance, Год журнала: 2023, Номер Volume 16, С. 3271 - 3292

Опубликована: Май 1, 2023

Staphylococcus aureus is a common human pathogen with variety of virulence factors, which can cause multiple infectious diseases. In recent decades, due to the constant evolution and abuse antibiotics, was becoming more resistant, infection rate MRSA remained high, clinical treatment became difficult. The genetic diversity mainly represented by continuous emergence epidemic strains, resulting in changes clones. Different classes resulted different epidemics resistance characteristics, could affect symptoms treatments. had also spread from traditional hospitals community livestock environments, new clones established relationship between animals humans, promoting further MRSA. Since mechanism very complex, it important clarify these mechanisms at molecular level for We firstly described SCCmec elements, discussed types SCCmec, its drug expression regulations. Then, we how vanA operon makes resistant vancomycin regulation. Finally, brief introduction given biofilms efflux pump systems. Analyzing help study anti-infective drugs alleviate At end review, summarized strategies infection, including anti-biofilm agents inhibitors. To sum up, here reviewed characteristics aureus, classifications, (SCCmec element, operon, biofilm active system) novel therapy strategies, so as provide theoretical basis infection.

Язык: Английский

Процитировано

29
Dynamic Protein Phosphorylation in Streptococcus pyogenes during Growth, Stationary Phase, and Starvation DOI Creative Commons
Stefan Mikkat, Michael Kreutzer, Nadja Patenge

и другие.

Microorganisms, Год журнала: 2024, Номер 12(3), С. 621 - 621

Опубликована: Март 20, 2024

Phosphorylation of proteins at serine, threonine, and tyrosine residues plays an important role in physiological processes bacteria, such as cell cycle, metabolism, virulence, dormancy, stationary phase functions. Little is known about the targets dynamics protein phosphorylation Streptococcus pyogenes, which possesses a single transmembrane serine/threonine kinase belonging to class PASTA kinases. A proteomics phosphoproteomics workflow was performed with S. pyogenes serotype M49 under different growth conditions, phase, starvation. The quantitative analysis dynamic phosphorylation, included subset 463 out 815 identified sites, revealed two main types events. small group events occurred almost exclusively threonine related cycle enhanced growing cells. majority during or starvation, preferentially serine residues. kinase-dependent regulation found bacteria are conserved pyogenes. Increased has also been described for some other could therefore be general feature physiology whose functions kinases involved need elucidated further analyses.

Язык: Английский

Процитировано

6
Cuminaldehyde inhibits biofilm formation by affecting the primary adhesion of Staphylococcus aureus DOI
Hui Li, Keren Agyekumwaa Addo,

Yi-gang Yu

и другие.

South African Journal of Botany, Год журнала: 2023, Номер 156, С. 13 - 20

Опубликована: Март 6, 2023

Язык: Английский

Процитировано

11
Efflux pump modulation by Montelukast and its roles in restoring antibiotic susceptibility in multidrug-resistant Staphylococcus aureus DOI Creative Commons
Shreesh Ojha, Simran Sinsinwar, P. Chatterjee

и другие.

EBioMedicine, Год журнала: 2025, Номер 114, С. 105658 - 105658

Опубликована: Апрель 1, 2025

Staphylococcus aureus and its drug-resistant mutants are mentioned among the WHO's high-priority list of pathogens. Antibiotics like fluoroquinolones cephalosporins used to treat multidrug-resistant S. infections. However, a higher expression efflux pumps (NorA, NorB, AbcA) induces multidrug resistance. The master regulator, MgrA, regulates most these in aureus. phosphorylation status MgrA is determined by cellular PknB/RsbU ratio, where PknB, serine-threonine kinase, RsbU, phosphatase, critical for functioning. An FDA-approved drug library was screened using an EtBr-accumulation assay identify pump inhibitors (EPIs). synergy EPIs with antibiotics studied vitro vivo murine skin infection model female BALB/c mice. effect on mgrA, norB, pknB, rsbU gene expression, interaction effects were studied. We identified Montelukast as effective EPI, which showed moxifloxacin, substrate NorB pump, both model. Further, decreased norB increased pknB/rsbU ratio. Our results demonstrated that strongly interacted facilitated phosphorylation, enhanced affinity promoter. study repressed promoted suppress activity, leading restoration antibiotic susceptibility supported SERB-DST, India (CRG/2021/005069), BRIC-ILS core.

Язык: Английский

Процитировано

0
Dividing lines: compartmentalisation and division in Streptomyces DOI Creative Commons
Matthew J. Bush, Bastien Casu, Susan Schlimpert

и другие.

Current Opinion in Microbiology, Год журнала: 2025, Номер 85, С. 102611 - 102611

Опубликована: Апрель 28, 2025

Bacteria display diverse strategies for cell division, exemplified by the multicellular life cycle of Streptomyces, a genus within Actinomycetota phylum. Filamentous growing Streptomyces utilise two distinct division modes: during vegetative growth, nonconstricting cross-walls divide mycelial network into long multinucleate compartments, while reproductive sporulation septation results in 'multiple event' that produces dozens unigenomic spores can separate and disperse environment. The cellular mechanisms governing these types are inherently complex present specific biological challenges involve core proteins several genus-specific factors. This review highlights recent advances open questions our understanding biology, with focus on key components interplay chromosome machinery, enabling organisms to grow as filaments form unicellular spores.

Язык: Английский

Процитировано

0
Evolution and classification of Ser/Thr phosphatase PP2C family in bacteria: Sequence conservation, structures, domain distribution DOI Creative Commons
Hang Li, Rui Li,

Haoyue Yu

и другие.

PLoS ONE, Год журнала: 2025, Номер 20(5), С. e0322880 - e0322880

Опубликована: Май 19, 2025

Serine/threonine kinases (STKs) and serine/threonine phosphatases (STPs) are widely present across various organisms play crucial roles in regulating cellular processes such as growth, proliferation, signal transduction, other physiological functions. Recent research has increasingly focused on the regulation of STKs STPs bacteria. have been well studied, identified characterized a variety bacterial species. However, role bacteria remains less understood, number proteins is limited. It found that most were Mg 2+ /Mn dependent 2C protein (PP2Cs), but evolutionary relationship taxonomic distribution PP2C still not fully elucidated. In this study, we utilized phosphatase sequences from InterPro database to perform phylogenetic analysis, categorizing family into five groups. Based classification, examined relationships, species distribution, sequence structural variations, domain characteristics phosphatases. Our analysis uncovered evidence common origin for These findings advance understanding phosphatases, offering valuable insights future functional studies aiding design targeted therapeutics pathogenic

Язык: Английский

Процитировано

0
Loratadine Combats Methicillin-Resistant Staphylococcus aureus by Modulating Virulence, Antibiotic Resistance, and Biofilm Genes DOI Creative Commons

Brianna Viering,

Halie Balogh,

Chloe F. Cox

и другие.

ACS Infectious Diseases, Год журнала: 2023, Номер 10(1), С. 232 - 250

Опубликована: Дек. 28, 2023

Methicillin-resistant Staphylococcus aureus (MRSA) has evolved to become resistant multiple classes of antibiotics. New antibiotics are costly develop and deploy, they have a limited effective lifespan. Antibiotic adjuvants molecules that potentiate existing through nontoxic mechanisms. We previously reported loratadine, the active ingredient in Claritin, potentiates cell-wall vitro disrupts biofilm formation hypothesized inhibition master regulatory kinase Stk1. Loratadine oxacillin combined repressed expression key antibiotic resistance genes bla mec operons. additional involved resistance, formation, other cellular pathways would be modulated when looking transcriptome-wide. To test this, we used RNA-seq quantify transcript levels found significant effects gene expression, including controlling virulence, metabolism, transcription (core RNA polymerase subunits sigma factors), translation (a plethora encoding ribosomal proteins elongation factor Tu). further demonstrated impacts these transcriptional by investigating loratadine treatment on intracellular ATP levels, persister morphology. minimized enhanced survival infected Caenorhabditis elegans. These pleiotropic their outcomes MRSA virulence phenotypes position as an attractive anti-infective against MRSA.

Язык: Английский

Процитировано

6
Experimental measurement and computational prediction of bacterial Hanks‐type Ser/Thr signaling system regulatory targets DOI Creative Commons

Noam Grunfeld,

Erel Levine, Elizabeth A. Libby

и другие.

Molecular Microbiology, Год журнала: 2024, Номер 122(2), С. 152 - 164

Опубликована: Янв. 3, 2024

Bacteria possess diverse classes of signaling systems that they use to sense and respond their environments execute properly timed developmental transitions. One widespread evolutionarily ancient class are the Hanks-type Ser/Thr kinases, also sometimes termed "eukaryotic-like" due homology with eukaryotic kinases. In bacterial species, these function as critical regulators general cellular processes such metabolism, growth division, transitions sporulation, biofilm formation, virulence, well antibiotic tolerance. This multifaceted regulation is ability a single kinase post-translationally modify activity multiple proteins, resulting in coordinated pathways. However, part deep integration physiology, date, we have relatively limited understanding timing, regulatory hierarchy, complete list targets given kinase, potential overlap between often kinases present organism. this review, discuss experimental methods curated datasets aimed at elucidating pathways approaches for using develop computational models quantitative predictions target motifs. We emphasize novel opportunities collecting data suitable creation new predictive applicable species.

Язык: Английский

Процитировано

2
The unusual mode of action of the polyketide glycoside antibiotic cervimycin C DOI Creative Commons
Alina Hoffmann,

Ursula Steffens,

Boris Maček

и другие.

mSphere, Год журнала: 2024, Номер 9(5)

Опубликована: Май 9, 2024

Cervimycins A-D are bis-glycosylated polyketide antibiotics produced by

Язык: Английский

Процитировано

2
GpsB coordinates StkP signaling as a PASTA kinase adaptor in Streptococcus pneumoniae cell division DOI Creative Commons

Václava Stauberová,

Bohumil Kubeša,

Merrin Joseph

и другие.

Journal of Molecular Biology, Год журнала: 2024, Номер unknown, С. 168797 - 168797

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

1