bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 5, 2024
Abstract
Background
Perspective
regenerative
therapies
for
injured
myocardium
focus
on
reactivating
developmental
processes
to
regenerate
damaged
contractile
tissue.
In
animal
models,
the
Hippo
pathway
was
shown
improve
heart
regeneration
after
myocardial
infarction,
possibly
by
expanding
pool
of
cardiomyocytes.
We
hypothesized
that
activating
pathway’s
downstream
effector,
Yes
Associated
Protein
(YAP1),
may
have
effects
beyond
promoting
proliferation
in
human
therefore
investigated
how
YAP1
influences
cardiomyocyte
maturation,
sarcomere
structure,
electrophysiological
properties,
their
response
mechanical
stimuli,
intracellular
Ca
2+
″
dynamics
and
force
development
using
models
cardiomyocytes
derived
from
pluripotent
stem
cells.
Methods
employed
genetic
deficient
embryonic
induced
cells,
differentiation,
compliant
cultivation
substrates,
actuation,
ECM
deposition,
super
resolution
microscopy,
measurements
engineered
tissues
(EHTs)
characterize
deficiency
during
maturation.
also
used
full
length
re-
expression
rescue
contracting
Results
contributes
participates
formation
alignment
myofibrils,
as
well
maturation
properties.
The
net
effect
is
inability
respond
physiological
stimuli
compensatory
growth
resulting
reduced
development.
Additionally,
reactivation
leads
myofibril
Conclusions
This
research
demonstrates
activity
essential
promote
contractility,
inducing
stimuli.
Cells,
Год журнала:
2023,
Номер
12(24), С. 2794 - 2794
Опубликована: Дек. 8, 2023
Gene
expression
is
under
tight
regulation
from
the
chromatin
structure
that
regulates
gene
accessibility
by
transcription
machinery
to
protein
degradation.
At
transcript
level,
this
falls
on
RNA-binding
proteins
(RBPs).
RBPs
are
a
large
and
diverse
class
of
involved
in
all
aspects
transcript’s
lifecycle:
splicing
maturation,
localization,
stability,
translation.
In
past
few
years,
our
understanding
role
cardiovascular
diseases
has
expanded.
Here,
we
discuss
general
function
latest
discoveries
their
pulmonary
systemic
diseases.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(20), С. 15418 - 15418
Опубликована: Окт. 21, 2023
Liquid-liquid
phase
separation
(LLPS)
is
a
biophysical
process
that
mediates
the
precise
and
complex
spatiotemporal
coordination
of
cellular
processes.
Proteins
nucleic
acids
are
compartmentalized
into
micron-scale
membrane-less
droplets
via
LLPS.
These
droplets,
termed
biomolecular
condensates,
highly
dynamic,
have
concentrated
components,
perform
specific
functions.
Biomolecular
condensates
been
observed
to
organize
diverse
key
biological
processes,
including
gene
transcription,
signal
transduction,
DNA
damage
repair,
chromatin
organization,
autophagy.
The
dysregulation
these
activities
owing
aberrant
LLPS
important
in
cardiovascular
diseases.
This
review
provides
detailed
overview
regulation
functions
comprehensive
depiction
several
common
diseases,
discusses
revolutionary
therapeutic
perspective
modulating
diseases
new
treatment
strategies
relevant
Current Opinion in Cell Biology,
Год журнала:
2023,
Номер
86, С. 102304 - 102304
Опубликована: Дек. 18, 2023
Elevated
tissue
stiffness
is
a
common
feature
of
many
solid
tumors
and
the
downstream
mechanical
signaling
affects
cellular
processes
contributes
to
cancer
progression.
Significant
progress
has
been
made
in
understanding
how
properties
matrix
affect
cell
behavior
as
well
transcription.
However,
same
cues
impact
protein
synthesis
stability
this
may
contribute
disease
less
understood.
Here,
we
present
emerging
evidence
that
progression
frequently
supported
by
gene
regulation
acting
beyond
mRNA
level
highlight
some
known
crosstalk
between
type
mechanotransduction
other
contexts.
We
suggest
future
systematic
approaches
define
mechanosensitive
translatomes
proteomes
these
are
controlled
provide
novel
targets
for
therapy.
Fibrosis
is
a
pathological
process
characterized
by
the
excessive
deposition
of
extracellular
matrix
in
tissue's
space,
leading
to
structural
injury
and
organ
dysfunction,
even
failure,
posing
threat
human
life.
Despite
mounting
evidence
suggesting
that
fibrosis
reversible,
effective
treatments
for
fibrotic
diseases
are
lacking.
Accumulating
has
elucidated
ribonucleic
acid
(RNA)
modifications
have
emerged
as
novel
mechanisms
regulating
gene
expression.
N6‐methyladenosine
(m6A)
modification
well‐known
prevalent
RNA
posttranscriptional
participates
essential
biological
processes
such
splicing,
translation,
degradation.
It
tightly
implicated
wide
range
cellular
various
diseases,
particularly
fibrosis.
The
m6A
dynamic
reversible
regulated
methylases,
commonly
known
“writers,”
demethylases
referred
“erasers,”
while
recognized
“readers.”
suggests
on
RNAs
associated
with
visceral
organs
including
lungs,
heart,
liver,
kidney.
In
this
review,
recent
advances
impact
methylation
highlighted
potential
prospects
therapy
treating
discussed.
Aging and Disease,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Reduced
adipogenesis
is
a
prominent
characteristic
of
aging
adipose
tissue
and
closely
tied
to
the
development
metabolic
disorders
associated
with
aging.
Epigenetic
modification
plays
crucial
role
in
process,
yet
N6-methyladenosine
(m6A),
most
prevalent
RNA
modification,
regulating
remains
uncertain.
Our
study
found
that
levels
m6A
its
recognition
protein,
heterogeneous
nuclear
ribonucleoprotein
C
(HNRNPC),
decrease
as
individuals
age.
Lower
HNRNPC
were
also
linked
reduced
during
Through
loss
gain
function
experiments
HNRNPC,
we
established
positive
correlation
between
vitro.
Hnrnpc-APKO
mice
displayed
decreased
adipogenesis,
increased
insulin
resistance,
elevated
expression
aging-related
inflammation-related
genes,
lipogenesis-related
other
compared
their
littermates.
Additionally,
discovered
facilitated
stability
lymphocyte
cytosolic
protein
1
(Lcp1)
mRNA
by
binding
motif
LCP1.
Overexpression
LCP1
mitigated
inhibition
caused
through
modulation
cytoskeletal
remodeling.
Finally,
our
findings
demonstrate
anti-aging
treatments
could
enhance
levels.
In
conclusion,
positively
aging,
increacing
highlights
potential
therapeutic
target
for
addressing
imbalances
related
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 5, 2024
Abstract
Background
Perspective
regenerative
therapies
for
injured
myocardium
focus
on
reactivating
developmental
processes
to
regenerate
damaged
contractile
tissue.
In
animal
models,
the
Hippo
pathway
was
shown
improve
heart
regeneration
after
myocardial
infarction,
possibly
by
expanding
pool
of
cardiomyocytes.
We
hypothesized
that
activating
pathway’s
downstream
effector,
Yes
Associated
Protein
(YAP1),
may
have
effects
beyond
promoting
proliferation
in
human
therefore
investigated
how
YAP1
influences
cardiomyocyte
maturation,
sarcomere
structure,
electrophysiological
properties,
their
response
mechanical
stimuli,
intracellular
Ca
2+
″
dynamics
and
force
development
using
models
cardiomyocytes
derived
from
pluripotent
stem
cells.
Methods
employed
genetic
deficient
embryonic
induced
cells,
differentiation,
compliant
cultivation
substrates,
actuation,
ECM
deposition,
super
resolution
microscopy,
measurements
engineered
tissues
(EHTs)
characterize
deficiency
during
maturation.
also
used
full
length
re-
expression
rescue
contracting
Results
contributes
participates
formation
alignment
myofibrils,
as
well
maturation
properties.
The
net
effect
is
inability
respond
physiological
stimuli
compensatory
growth
resulting
reduced
development.
Additionally,
reactivation
leads
myofibril
Conclusions
This
research
demonstrates
activity
essential
promote
contractility,
inducing
stimuli.
