Merkel cell stimulation in fear and sensory signaling DOI Creative Commons
Austin C. Korgan, Rodrigo Orso,

Isabelle J. Sibley

и другие.

Neuropsychopharmacology, Год журнала: 2025, Номер unknown

Опубликована: Июнь 7, 2025

Abstract Stress and traumatic experiences have significant lasting effects on sensory systems. We recently identified unique expression of proteins associated with epidermal skin cells (keratinocytes) mechanosensory Merkel (MC) in circulating extracellular vesicles from adult women who had experienced sexual trauma specifically during adolescence, biologically linking exposure a specific neuron-like cell. Here, we aimed to develop validate preclinical mouse model utilizing chemogenetic (DREADD Gq) activation population MC. Using reporter line, confirmed the expected pattern Krt14 Cre MC areas that these tissues expressed relevant marker genes similarly between male female mice. Chemogenetic stimulation produced robust neuronal insular cortex (IC), brain region somatosensory valence integration. To determine if mice could detect activation, home cage behaviors following CNO treatment significantly increased nest grooming time. Conditioned place preference further revealed an avoidance response stimulation; effect was stronger Finally, connect back our question, examined fear conditioning deficits extinction. Overall, studies utilization this investigating system its potential involvement PTSD symptoms therapeutic interventions. Ongoing will focus critical developmental periods both development sex differences vulnerability based options for PTSD-related symptoms.

Язык: Английский

TRPV4 activation in Schwann cells mediates mechanically induced pain of oral cancer DOI Creative Commons
Yatendra Mulpuri, Nguyen Huu Tu, Kenji Inoue

и другие.

Frontiers in Pain Research, Год журнала: 2025, Номер 6

Опубликована: Март 12, 2025

Introduction Patients with oral cancer often experience intense functional pain due to mechanical stimulation at the site. The role of mechanosensitive ion channels in pain, such as TRPV4, is not fully understood. Objectives Our objective was investigate Schwann cell TRPV4 pain. Methods We examined impact inhibition on NU/J and C57BL/6J mice injected human tongue line (HSC-3) mouse (MOC2) hind paw or tongue. Mechanical heat sensitivity were assessed using von Frey Hargreaves tests, respectively. expression activity cells analyzed immunohistochemistry, qRT-PCR, Ca 2+ imaging, patch-clamp electrophysiology. effect activation responses evaluated a piezo stimulator. Conditioned media (CM) from TRPV4-activated into evaluate contribution hypersensitivity. Results reduced nociception dose-dependently without affecting sensitivity. also decreased facial mice. expressed mainly plasma membrane induced whole-cell currents cells. Mechanoactivated inhibited by antagonist HC-067047. CM hypersensitivity mice, which blocked pre-treatment Conclusion plays mediating mechanically cancer.

Язык: Английский

Процитировано

0
Epidermal cells and sensory neurons team up DOI Creative Commons
Jean-Christophe Boivin, Tomoko Ohyama

eLife, Год журнала: 2025, Номер 14

Опубликована: Май 12, 2025

Experiments in fruit fly larvae show that epidermal cells can communicate with sensory neurons to drive responses pain.

Язык: Английский

Процитировано

0
Merkel cell stimulation in fear and sensory signaling DOI Creative Commons
Austin C. Korgan, Rodrigo Orso,

Isabelle J. Sibley

и другие.

Neuropsychopharmacology, Год журнала: 2025, Номер unknown

Опубликована: Июнь 7, 2025

Abstract Stress and traumatic experiences have significant lasting effects on sensory systems. We recently identified unique expression of proteins associated with epidermal skin cells (keratinocytes) mechanosensory Merkel (MC) in circulating extracellular vesicles from adult women who had experienced sexual trauma specifically during adolescence, biologically linking exposure a specific neuron-like cell. Here, we aimed to develop validate preclinical mouse model utilizing chemogenetic (DREADD Gq) activation population MC. Using reporter line, confirmed the expected pattern Krt14 Cre MC areas that these tissues expressed relevant marker genes similarly between male female mice. Chemogenetic stimulation produced robust neuronal insular cortex (IC), brain region somatosensory valence integration. To determine if mice could detect activation, home cage behaviors following CNO treatment significantly increased nest grooming time. Conditioned place preference further revealed an avoidance response stimulation; effect was stronger Finally, connect back our question, examined fear conditioning deficits extinction. Overall, studies utilization this investigating system its potential involvement PTSD symptoms therapeutic interventions. Ongoing will focus critical developmental periods both development sex differences vulnerability based options for PTSD-related symptoms.

Язык: Английский

Процитировано

0