TRPV4 activation in Schwann cells mediates mechanically induced pain of oral cancer
Frontiers in Pain Research,
Год журнала:
2025,
Номер
6
Опубликована: Март 12, 2025
Introduction
Patients
with
oral
cancer
often
experience
intense
functional
pain
due
to
mechanical
stimulation
at
the
site.
The
role
of
mechanosensitive
ion
channels
in
pain,
such
as
TRPV4,
is
not
fully
understood.
Objectives
Our
objective
was
investigate
Schwann
cell
TRPV4
pain.
Methods
We
examined
impact
inhibition
on
NU/J
and
C57BL/6J
mice
injected
human
tongue
line
(HSC-3)
mouse
(MOC2)
hind
paw
or
tongue.
Mechanical
heat
sensitivity
were
assessed
using
von
Frey
Hargreaves
tests,
respectively.
expression
activity
cells
analyzed
immunohistochemistry,
qRT-PCR,
Ca
2+
imaging,
patch-clamp
electrophysiology.
effect
activation
responses
evaluated
a
piezo
stimulator.
Conditioned
media
(CM)
from
TRPV4-activated
into
evaluate
contribution
hypersensitivity.
Results
reduced
nociception
dose-dependently
without
affecting
sensitivity.
also
decreased
facial
mice.
expressed
mainly
plasma
membrane
induced
whole-cell
currents
cells.
Mechanoactivated
inhibited
by
antagonist
HC-067047.
CM
hypersensitivity
mice,
which
blocked
pre-treatment
Conclusion
plays
mediating
mechanically
cancer.
Язык: Английский
Epidermal cells and sensory neurons team up
eLife,
Год журнала:
2025,
Номер
14
Опубликована: Май 12, 2025
Experiments
in
fruit
fly
larvae
show
that
epidermal
cells
can
communicate
with
sensory
neurons
to
drive
responses
pain.
Язык: Английский
Merkel cell stimulation in fear and sensory signaling
Neuropsychopharmacology,
Год журнала:
2025,
Номер
unknown
Опубликована: Июнь 7, 2025
Abstract
Stress
and
traumatic
experiences
have
significant
lasting
effects
on
sensory
systems.
We
recently
identified
unique
expression
of
proteins
associated
with
epidermal
skin
cells
(keratinocytes)
mechanosensory
Merkel
(MC)
in
circulating
extracellular
vesicles
from
adult
women
who
had
experienced
sexual
trauma
specifically
during
adolescence,
biologically
linking
exposure
a
specific
neuron-like
cell.
Here,
we
aimed
to
develop
validate
preclinical
mouse
model
utilizing
chemogenetic
(DREADD
Gq)
activation
population
MC.
Using
reporter
line,
confirmed
the
expected
pattern
Krt14
Cre
MC
areas
that
these
tissues
expressed
relevant
marker
genes
similarly
between
male
female
mice.
Chemogenetic
stimulation
produced
robust
neuronal
insular
cortex
(IC),
brain
region
somatosensory
valence
integration.
To
determine
if
mice
could
detect
activation,
home
cage
behaviors
following
CNO
treatment
significantly
increased
nest
grooming
time.
Conditioned
place
preference
further
revealed
an
avoidance
response
stimulation;
effect
was
stronger
Finally,
connect
back
our
question,
examined
fear
conditioning
deficits
extinction.
Overall,
studies
utilization
this
investigating
system
its
potential
involvement
PTSD
symptoms
therapeutic
interventions.
Ongoing
will
focus
critical
developmental
periods
both
development
sex
differences
vulnerability
based
options
for
PTSD-related
symptoms.
Язык: Английский