Geomicrobiology Journal,
Год журнала:
2024,
Номер
41(5), С. 584 - 593
Опубликована: Май 2, 2024
Stable
and
efficient
partial
nitrification
reaction
nitrite
accumulation
are
essential
for
the
operation
of
nitrification-anaerobic
ammonia
oxidation
process.
This
paper
investigated
changes
in
performance
a
bioreactor
during
domestication
After
40
days
domestication,
remained
stable,
rate
(NAR)
increased
significantly
to
85.7%,
NO2--N/NH4+-N
ratio
stabilized
at
1.25
±
0.04.
Both
diversity
indices
(Simpson
Shannon)
richness
(Ace
Chao1)
tend
decrease
with
domestication.
Nitrosomonas
as
typical
oxidizing
bacterium
it
its
relative
abundance
reactor
from
0.02
0.007%
P1
11.6
2.2%
P2,
P3
stage
denitrifying
functional
bacteria
norank_f_AKYH76,
Dokdonella
unclassified_f_Comamonadaceae
reached
33.4
12.5%,
18.8
1.8%,
10.1
1.3%,
respectively,
increase
these
species
was
also
consistent
nitrogen
reactor.
Abstract
The
discovery
of
comammox
Nitrospira
in
low
pH
environments
has
reshaped
the
ammonia
oxidation
process
acidic
settings,
providing
a
plausible
explanation
for
higher
nitrification
rates
observed
weakly
soils.
However,
response
to
varying
levels
and
its
ecological
role
these
remains
unclear.
Here,
survey
across
soils
with
values
(ranging
from
4.4
9.7)
was
conducted
assess
how
perform
under
different
conditions.
Results
showed
that
dominate
soils,
functioning
as
K‐strategy
species
characterized
by
slow
growth
stress
tolerance.
As
key
this
environment,
may
promote
bacterial
cooperation
Genomic
evidence
suggested
cobalamin
sharing
is
potential
mechanism,
uniquely
encode
metabolic
pathway
compensates
imbalance
where
86.8%
metagenome‐assembled
genomes
(MAGs)
cobalamin‐dependent
genes.
Additionally,
we
used
DNA
stable‐isotope
probing
(DNA‐SIP)
demonstrate
fluctuations
reflect
it
responds
decrease
pH.
confirmed
became
dominant
oxidizers
soil
after
We
will
become
increasingly
important
global
trend
acidification.
Overall,
our
work
provides
insights
changes.
Applied and Environmental Microbiology,
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 13, 2024
Nitrification
is
a
key
process
in
the
global
nitrogen
cycle.
Aerobic
ammonia
oxidizers
play
central
role
cycle
by
performing
first
step
of
nitrification.
Ammonia-oxidizing
archaea
(AOA)
and
complete
(comammox)
are
dominant
nitrifiers
environments
with
low
ammonium
availability.
While
AOA
have
been
studied
for
almost
20
years,
comammox
were
only
discovered
8
years
ago.
Until
now,
there
has
gap
our
understanding
whether
can
co-exist
or
if
one
strain
would
be
under
ammonium-limiting
conditions.
Here,
we
present
study
characterizing
competition
between
freshwater
varying
substrate
concentrations.
Our
results
will
help
elucidating
niches
two
lakes.
Applied Microbiology and Biotechnology,
Год журнала:
2024,
Номер
108(1)
Опубликована: Окт. 24, 2024
Nitrification
is
a
vital
process
in
the
biological
removal
of
inorganic
nitrogen
compounds.
In
order
to
ensure
stability
and
effectiveness
this
process,
buffer
solutions
should
be
added
system
maintain
neutral
slightly
alkaline
conditions.
With
focus
on
newly
discovered
comammox
Nitrospira,
research
investigates
transition
nitrifying
community
within
biofilm
reactor
under
different
acidic
levels
(initiated
at
pH
6
gradually
decreased
5).
During
305-day
continuous
operation
experiment,
it
was
observed
that
responsible
ammonia
oxidizers
transitioned
from
ammonia-oxidizing
bacteria
(AOB)
during
initial
stages
(setup
stage
early
6)
Nitrospira
5.5
5.
Further
analysis
using
next-generation
sequencing
targeting
both
16S
rRNA
region
amoA
revealed
shift
dominant
cluster
Nitrospirae
varying
Our
study
identified
distinct
phylogenetically
closed
sequences
found
environments,
but
exhibits
dissimilarity
known
isolates
majority
environmental
sequences.
This
prevalent
studied
thrived
particularly
well
These
findings
underscore
potential
significance
distinct,
uncultivated
group
performing
oxidation
KEY
POINTS:
•
Ammonia
effectively
removed
5
The
oxidizer
when
A
acidophilic
reactor.
