Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Апрель 22, 2024

Poly(butylene adipate-co-terephthalate) (PBAT) is a polyester widely used in various fields and its waste gradually accumulates the environment, posing ecological risks. Enzymatic hydrolysis holds great potential end-of-life management of PBAT, but reported enzymes need to be incubated at high temperature (e.g. 70 °C), which supposed cost huge amount energy thereby limiting wide industrial application. Here, we found that marine fungus Alternaria alternata FB1 could efficiently degrade PBAT moderate (e. g. 37 °C). Two fungal cutinases (named AaCut4 AaCut10) were predicted determine degradation process by establishing hidden Markov model hydrolases, verified an vivo gene knockout method. The vitro expressed AaCut10 completely depolymerize film 48 h, whereas achieved 63.59% breakdown 96 h °C, no product inhibition either enzyme was detected. Targeting AaCut10, accomplished increase yield terephthalic acid monomer 96.01% °C through performing site-directed mutagenesis supplementing Ca2+ or Mg2+ reaction buffer. We also hydrolases broadly distributed different species environments, indicating their resolving plastics contamination. Overall, our study provides novel candidates for efficient decomposition mild paves way recover plastic wastes low consumption manner, important sustainable use waste.

Язык: Английский