Environmental Microbiology,
Год журнала:
2013,
Номер
15(8), С. 2275 - 2292
Опубликована: Янв. 29, 2013
The
dynamics
of
populations
and
activities
ammonia-oxidizing
nitrite-oxidizing
microorganisms
were
investigated
in
rice
microcosms
treated
with
two
levels
nitrogen.
Different
soil
compartments
(surface,
bulk,
rhizospheric
soil)
roots
(young
old
roots)
collected
at
three
time
points
(the
panicle
initiation,
heading
maturity
periods)
the
season.
population
bacterial
(AOB)
archaeal
(AOA)
ammonia
oxidizers
was
assayed
by
determining
abundance
(using
qPCR)
composition
T-RFLP
cloning/sequencing)
their
amoA
genes
(coding
for
a
subunit
monooxygenase),
that
nitrite
(NOB)
quantifying
nxrA
gene
oxidase
Nitrobacter
spp.)
16S
rRNA
Nitrospira
spp.
activity
nitrifiers
determined
measuring
rates
potential
oxidation
copy
numbers
transcripts.
Potential
much
higher
than
not
directly
affected
nitrogen
amendment
demonstrating
importance
as
pace
makers
oxidizer
populations.
Marked
differences
distribution
oxidizers,
Nitrobacter-like
Nitrospira-like
found
different
planted
paddy
indicating
niche
differentiation.
In
bulk
soil,
bacteria
(Nitrosospira
Nitrosomonas)
low
displayed
no
activity,
but
surface
high.
Nitrite
dominated
By
contrast,
Thaumarchaeota
seemed
to
dominate
nitrification
on
roots.
contrast
compartment,
level
N
fertilization
point
sampling
had
only
little
effect
abundance,
nitrifying
communities.
results
our
study
show
fields
is
mainly
differentiated
rather
or
Environmental Science & Technology,
Год журнала:
2015,
Номер
49(13), С. 7692 - 7700
Опубликована: Май 28, 2015
Polymetallic
nodules
(manganese
nodules)
have
been
formed
on
deep
sea
sediments
over
millions
of
years
and
are
currently
explored
for
their
economic
potential,
particularly
cobalt,
nickel,
copper,
manganese.
Here
we
microbial
communities
inside
from
the
northeastern
equatorial
Pacific.
The
a
large
connected
pore
space
with
huge
inner
surface
120
m2/g
as
analyzed
by
computer
tomography
BET
measurements.
X-ray
photoelectron
spectroscopy
(XPS)
electron
microprobe
analysis
revealed
complex
chemical
fine
structure.
This
consisted
layers
highly
variable
Mn/Fe
ratios
(<1
to
>500)
mainly
turbostratic
phyllomanganates
such
7
10
Å
vernadites
alternating
Fe-bearing
vernadite
(δ-MnO2)
epitaxially
intergrown
amorphous
feroxyhyte
(δ-FeOOH).
Using
molecular
16S
rRNA
gene
techniques
(clone
libraries,
pyrosequencing,
real-time
PCR),
show
that
polymetallic
provide
suitable
habitat
prokaryotes
an
abundant
diverse
prokaryotic
community
dominated
nodule-specific
Mn(IV)-reducing
Mn(II)-oxidizing
bacteria.
These
bacteria
were
not
detected
in
nodule-surrounding
sediment.
high
abundance
dominance
Mn-cycling
manganese
argue
biologically
driven
closed
cycle
relevant
formation
potential
degradation.
Environmental Microbiology,
Год журнала:
2013,
Номер
15(8), С. 2275 - 2292
Опубликована: Янв. 29, 2013
The
dynamics
of
populations
and
activities
ammonia-oxidizing
nitrite-oxidizing
microorganisms
were
investigated
in
rice
microcosms
treated
with
two
levels
nitrogen.
Different
soil
compartments
(surface,
bulk,
rhizospheric
soil)
roots
(young
old
roots)
collected
at
three
time
points
(the
panicle
initiation,
heading
maturity
periods)
the
season.
population
bacterial
(AOB)
archaeal
(AOA)
ammonia
oxidizers
was
assayed
by
determining
abundance
(using
qPCR)
composition
T-RFLP
cloning/sequencing)
their
amoA
genes
(coding
for
a
subunit
monooxygenase),
that
nitrite
(NOB)
quantifying
nxrA
gene
oxidase
Nitrobacter
spp.)
16S
rRNA
Nitrospira
spp.
activity
nitrifiers
determined
measuring
rates
potential
oxidation
copy
numbers
transcripts.
Potential
much
higher
than
not
directly
affected
nitrogen
amendment
demonstrating
importance
as
pace
makers
oxidizer
populations.
Marked
differences
distribution
oxidizers,
Nitrobacter-like
Nitrospira-like
found
different
planted
paddy
indicating
niche
differentiation.
In
bulk
soil,
bacteria
(Nitrosospira
Nitrosomonas)
low
displayed
no
activity,
but
surface
high.
Nitrite
dominated
By
contrast,
Thaumarchaeota
seemed
to
dominate
nitrification
on
roots.
contrast
compartment,
level
N
fertilization
point
sampling
had
only
little
effect
abundance,
nitrifying
communities.
results
our
study
show
fields
is
mainly
differentiated
rather
or
