Diversity, structure and convergent evolution of the global sponge microbiome

Nature Communications, Год журнала: 2016, Номер 7(1)

Опубликована: Июнь 16, 2016

Abstract Sponges (phylum Porifera) are early-diverging metazoa renowned for establishing complex microbial symbioses. Here we present a global Porifera microbiome survey, set out to establish the ecological and evolutionary drivers of these host–microbe interactions. We show that sponges reservoir exceptional diversity major contributors total world’s oceans. Little commonality in species composition or structure is evident across phylum, although symbiont communities characterized by specialists generalists rather than opportunists. Core sponge microbiomes stable generalist symbionts exhibiting amensal and/or commensal Symbionts phylogenetically unique do not disproportionally contribute core microbiome, host phylogeny impacts complexity community. Our findings support model independent assembly evolution entire with convergent forces resulting analogous community organization

Язык: Английский

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The sponge holobiont in a changing ocean: from microbes to ecosystems

Microbiome, Год журнала: 2018, Номер 6(1)

Опубликована: Март 9, 2018

The recognition that all macroorganisms live in symbiotic association with microbial communities has opened up a new field biology. Animals, plants, and algae are now considered holobionts, complex ecosystems consisting of the host, microbiota, interactions among them. Accordingly, ecological concepts can be applied to understand host-derived processes govern dynamics interactive networks within holobiont. In marine systems, holobionts further integrated into larger more ecosystems, concept referred as "nested ecosystems." this review, we discuss dynamic interact at multiple scales respond environmental change. We focus on symbiosis sponges their communities—a resulted one most diverse environment. recent years, sponge microbiology remarkably advanced terms curated databases, standardized protocols, information functions microbiota. Like Russian doll, these translated holobiont impact surrounding ecosystem. For example, sponge-associated metabolisms, fueled by high filtering capacity substantially affect biogeochemical cycling key nutrients like carbon, nitrogen, phosphorous. Since increasingly threatened anthropogenic stressors jeopardize stability ecosystem, link between perturbations, dysbiosis, diseases. Experimental studies suggest community composition is tightly linked health, but whether dysbiosis cause or consequence collapse remains unresolved. Moreover, potential role microbiome mediating for acclimate adapt change unknown. Future should aim identify mechanisms underlying scales, from develop management strategies preserve provided our present future oceans.

Язык: Английский

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Hemolymph microbiome of Pacific oysters in response to temperature, temperature stress and infection

The ISME Journal, Год журнала: 2014, Номер 9(3), С. 670 - 682

Опубликована: Сен. 2, 2014

Язык: Английский

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The Sponge Hologenome

mBio, Год журнала: 2016, Номер 7(2)

Опубликована: Апрель 22, 2016

A paradigm shift has recently transformed the field of biological science; molecular advances have revealed how fundamentally important microorganisms are to many aspects a host's phenotype and evolution. In process, an era "holobiont" research emerged investigate intricate network interactions between host its symbiotic microbial consortia. Marine sponges early-diverging metazoa known for hosting dense, specific, often highly diverse communities. Here we synthesize current thoughts about environmental evolutionary forces that influence diversity, specificity, distribution symbionts within sponge holobiont, explore physiological pathways contribute holobiont function, describe mechanisms underpin establishment maintenance these partnerships. The collective genomes form hologenome, highlight define sponge's in fact act on genomic interplay different components holobiont.

Язык: Английский

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Host-specificity among abundant and rare taxa in the sponge microbiome

The ISME Journal, Год журнала: 2014, Номер 8(6), С. 1198 - 1209

Опубликована: Янв. 9, 2014

Язык: Английский

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Coral-the world's most diverse symbiotic ecosystem

Molecular Ecology, Год журнала: 2015, Номер 24(21), С. 5330 - 5347

Опубликована: Сен. 28, 2015

Zooxanthellate corals (i.e. those harbouring Symbiodinium) are the main builders of world's shallow-water marine coral reefs. They represent intimate diverse symbioses between animals, single-celled photosynthetic dinoflagellates (Symbiodinium spp.), other microscopic eukaryotes, prokaryotes and viruses. Crabs crustaceans, worms, sponges, bivalves hydrozoans, fishes, sea urchins, octopuses stars itinerant members these 'rainforests sea'. This review focuses on biodiversity scleractinian animals their best studied epi- endosymbionts. In relation to coral-associated species diversity, Symbiodinium internal transcribed spacer region sequence types tally 10(2) -10(3) or up ~15 different operational taxonomic units (OTUs, putative at 97% identity level; this cut-off was chosen based intragenomic diversity observed in monoclonal cultures) (mostly bacterial) total -10(4) OTUs. We analysed all publically accessible 16S rRNA gene data found Gammaproteobacteria were extremely abundant, followed by Alphaproteobacteria. Notably, Archaea poorly represented 'unassigned OTUs' abundant generated high-throughput DNA sequencing studies corals. outline compare model systems that could be used future holobiont. our directions, we recommend a global sampling effort including substantial attention being paid method tissue acquisition, which compartments (mucus, tissue, skeleton) explore, broadening holobiont considered linking with functional investigations.

Язык: Английский

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Phylogenetic signal in the community structure of host-specific microbiomes of tropical marine sponges

Frontiers in Microbiology, Год журнала: 2014, Номер 5

Опубликована: Окт. 17, 2014

Sponges (Porifera) can host diverse and abundant communities of microbial symbionts that make crucial contributions to metabolism. Although these are often host-specific hypothesized co-evolve with their hosts, correlations between phylogeny microbiome community structure rarely tested. As part the Earth Microbiome Project, we surveyed microbiomes associated 20 species tropical marine sponges collected over a narrow geographic range. We tested whether (1) univariate metrics diversity displayed significant phylogenetic signal across phylogeny; (2) identity were factors in multivariate analyses taxonomic dissimilarity; (3) different minimum read thresholds impacted results. observed differences among for all thresholds, strong inverse Simpson's index (D). surprisingly wide range variability dissimilarity within (4% 73%); this was not related abundance species. Taxonomic significantly by when considered individually; together, effect reduced, but remained significant. In our dataset, outcome is largely due closely harboring distinct taxa. specific taxa varied substantially sponges, hosts tended harbor similar patterns relative abundance. hypothesize low D might be structured regulation or presence competitively dominant themselves under selection specificity.

Язык: Английский

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Microbial indicators as a diagnostic tool for assessing water quality and climate stress in coral reef ecosystems

Marine Biology, Год журнала: 2017, Номер 164(4)

Опубликована: Март 23, 2017

Язык: Английский

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Effects of environmental temperature on the gut microbial communities of tadpoles

Environmental Microbiology, Год журнала: 2016, Номер 18(5), С. 1561 - 1565

Опубликована: Март 5, 2016

Numerous studies have investigated the effects of diet, phylogeny and immune status on gut microbial communities animals. Most these are conducted endotherms, especially mammals, which maintain constant body temperature in face environmental variability. However, majority animals vertebrates ectotherms, often experience fluctuations as a result their environment. While there been several investigating diversity we lack an understanding how affects communities. Here, used high-throughput sequencing to inventory tadpoles exposed cool (18°C) or warm (28°C) treatments. We found that significantly impacted community structure membership tadpole gut. Specifically, treatment exhibited higher abundances phylum Planctomycetes genus Mycobacterium. These results may be due direct temperature, mediated through changes host physiology. Given temperatures expected increase global climate change, function is critical.

Язык: Английский

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Bacterial dynamics within the mucus, tissue and skeleton of the coral Porites lutea during different seasons

Scientific Reports, Год журнала: 2014, Номер 4(1)

Опубликована: Дек. 5, 2014

Investigation of the response coral microbial communities to seasonal ecological environment at microscale will advance our understanding relationship between coral-associated bacteria community and health. In this study, we examined composition from mucus, tissue skeleton Porites lutea surrounding seawater every three months for 1 year on Luhuitou fringing reef. The bacterial were analyzed using pyrosequencing V1-V2 region 16S rRNA gene, which demonstrated diverse consortium profiles in corals. all compartments studied significantly different seawater. Moreover, they had a much more dynamic compared communities. collected each sample tended cluster together. Analysis assemblages environmental parameters showed that correlated dissolved oxygen rainfall study site. This highlights high complexity associated seasonally varying ecosystem parameters.

Язык: Английский

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