Probiotics and Antimicrobial Proteins, Год журнала: 2022, Номер 16(1), С. 76 - 92
Опубликована: Дек. 2, 2022
Язык: Английский
Biofilm, Год журнала: 2024, Номер 7, С. 100194 - 100194
Опубликована: Март 28, 2024
is widely distributed in marine ecosystems. Magnesium ion (Mg
Язык: Английский
Процитировано
11
Frontiers in Microbiology, Год журнала: 2023, Номер 14
Опубликована: Фев. 9, 2023
Mature biofilm formation by Vibrio parahaemolyticus requires exopolysaccharide (EPS), type IV pili, and capsular polysaccharide (CPS). Production of each is strictly regulated various control pathways including quorum sensing (QS) bis-(3′–5′)-cyclic di-GMP (c-di-GMP). QsvR, an AraC-type regulator, integrates into the QS regulatory cascade via direct transcription master regulators, AphA OpaR. Deletion qsvR in wild-type or opaR mutant backgrounds altered V. , suggesting that QsvR may coordinate with OpaR to formation. Herein, we demonstrated both repressed biofilm-associated phenotypes, c-di-GMP metabolism, translucent (TR) colonies. restored phenotypic changes caused mutation, vice versa. In addition, worked coordinately regulate EPS-associated genes, pili CPS genes metabolism-related genes. These results how works system precisely controlling multiple formation-associated .
Язык: Английский
Процитировано
22
Frontiers in Microbiology, Год журнала: 2023, Номер 14
Опубликована: Сен. 26, 2023
Vibrio parahaemolyticus isolates are generally very sensitive to chloramphenicol. However, it is usually necessary transfer a plasmid carrying chloramphenicol resistance gene into V. investigate the function of specific gene, and effects on bacterial physiology have not been investigated. In this work, sublethal dose were investigated by combined utilization various phenotypic assays RNA sequencing (RNA-seq). The results showed that growth rate, biofilm formation capcity, c-di-GMP synthesis, motility, cytoxicity adherence activity remarkably downregulated RNA-seq data revealed expression levels 650 genes significantly differentially expressed in response stress, including antibiotic genes, major virulence biofilm-associated putative regulatory genes. Majority involved synthesis polar flagellum, exopolysaccharide (EPS), mannose-sensitive haemagglutinin type IV pilus (MSHA), III secretion systems (T3SS1 T3SS2) VI system 2 (T6SS2) Five metabolism expressed, which may be reason for decrease intracellular stress. addition, 23 encoding regulators also suggesting these This work helps us understand how effect .
Язык: Английский
Процитировано
18
Gut Microbes, Год журнала: 2024, Номер 16(1)
Опубликована: Март 11, 2024
Although metals are essential for life, they toxic to bacteria in excessive amounts. Therefore, the maintenance of metal homeostasis is critical bacterial physiology and pathogenesis. Vibrio parahaemolyticus a significant food-borne pathogen that mainly causes acute gastroenteritis humans hepatopancreatic necrosis disease shrimp. Herein, we report ZntA functions as zinc (Zn) cadmium (Cd) mechanism contributes oxidative stress resistance virulence V. parahaemolyticus. zntA remarkably induced by Zn, copper, cobalt, nickel (Ni), Cd, while promotes growth under excess Zn/Ni Cd conditions via maintaining Zn homeostasis, respectively. The ΔzntA was inhibited iron (Fe)-restricted conditions, inhibition associated with disturbance. Ferrous supplementation improved Ni or conditions. H2O2-induced also decreased, its attenuated zebrafish models. Quantitative real-time PCR, mutagenesis, β-galactosidase activity assays revealed ZntR positively regulates expression binding promoter. Collectively, ZntR-regulated crucial
Язык: Английский
Процитировано
8
Journal of Bacteriology, Год журнала: 2023, Номер 205(9)
Опубликована: Сен. 1, 2023
The L-arabinose inducible pBAD vectors are commonly used to turn on and off the expression of specific genes in bacteria. utilization certain carbohydrates can influence bacterial growth, virulence factor production, biofilm formation. Vibrio parahaemolyticus, causative agent seafood-associated gastroenteritis, grow media with as sole carbon source. However, effects V. parahaemolyticus physiology have not been investigated. In this study, we show that growth rate, formation capacity, capsular polysaccharide motility, c-di-GMP production negatively affected by L-arabinose. RNA-seq data revealed significant changes levels 752 genes, accounting for approximately 15.6% presence included those associated utilization, major known key biofilm-related numerous regulatory genes. majority type III secretion system, two were upregulated L-arabinose, whereas VI downregulated. Ten putative metabolism-associated also significantly differentially expressed, which may account reduced Most importantly, almost 40 regulators expressed due induction indicating is strictly regulated networks parahaemolyticus. findings increase understanding how affects Researchers should use caution when considering IMPORTANCE study formation, clarify gene profiles bacterium Significantly response involved multiple cellular pathways, including adaptation, regulation. collective indicate impact There be similar other species Necessary controls established must ectopic expression.
Язык: Английский
Процитировано
16
Food Bioscience, Год журнала: 2024, Номер 60, С. 104283 - 104283
Опубликована: Май 7, 2024
Язык: Английский
Процитировано
5
International Journal of Biological Macromolecules, Год журнала: 2023, Номер 239, С. 124355 - 124355
Опубликована: Апрель 5, 2023
Язык: Английский
Процитировано
12
Current Microbiology, Год журнала: 2023, Номер 80(12)
Опубликована: Окт. 14, 2023
Язык: Английский
Процитировано
11
International Journal of Biological Macromolecules, Год журнала: 2024, Номер 261, С. 129857 - 129857
Опубликована: Фев. 1, 2024
Язык: Английский
Процитировано
4
Archives of Microbiology, Год журнала: 2025, Номер 207(2)
Опубликована: Янв. 9, 2025
Язык: Английский
Процитировано
0