bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 21, 2024
Abstract
DPANN
is
a
widespread
and
highly
diverse
group
of
archaea
characterised
by
their
small
size,
reduced
genome,
limited
metabolic
pathways,
symbiotic
existence.
Known
species
are
predominantly
obligate
ectosymbionts
that
depend
on
host
for
survival
proliferation.
Despite
the
recent
expansion
in
this
clade,
structural
molecular
details
recognition,
host-DPANN
intercellular
communication,
adaptation
response
to
attachment
remain
unknown.
Here,
we
used
electron
cryotomography
(cryo-ET)
reveal
Candidatus
Micrarchaeota
(ARM-1)
interacts
with
its
host,
Metallosphaera
javensis
through
proteinaceous
nanotubes.
These
tubes
(∼4.5
nm
wide)
originate
extend
all
way
cytoplasm
act
like
tunnels
exchange.
Combining
cryo-ET
sub-tomogram
averaging,
revealed
situ
architectures
S-layers
structures
nanotubes
primed
extended
states,
providing
mechanistic
insights
into
substrate
Additionally,
performed
comparative
proteomics
genomic
analyses
identify
proteomic
changes
attachment.
Our
results
showed
striking
alterations
host-proteome
during
symbiosis
upregulation/downregulation
key
cellular
pathways.
Collectively,
these
provided
unprecedented
basis
communication
deepen
our
understanding
ectosymbiotic
relationships.
Although
microbial
colonization
of
the
internal
tissues
animals
generally
causes
septicemia
and
death,
various
are
persistently
associated
with
benign
or
beneficial
microorganisms
in
their
blood
organs.
The
metabolic
consequences
these
persistent
associations
for
animal
host
largely
unknown.
Our
research
on
facultative
bacterium
Hamiltonella
,
localized
primarily
to
hemolymph
pea
aphids,
demonstrated
that
although
imposed
no
major
reconfiguration
aphid
metabolome,
it
did
alter
relations
between
its
obligate
intracellular
symbiont,
Buchnera
.
Specifically,
produced
more
histidine
Hamiltonella-
positive
aphids
support
both
demand
-induced
increase
demand.
This
study
demonstrates
how
can
influence
specific
aspects
interactions
co-occurring
microorganisms.
Current Research in Insect Science,
Год журнала:
2023,
Номер
5, С. 100070 - 100070
Опубликована: Дек. 20, 2023
Aphids
are
valuable
models
for
studying
the
functional
diversity
of
bacterial
symbiosis
in
insects.
In
addition
to
their
ancestral
obligate
nutritional
symbiont
Microbiology Spectrum,
Год журнала:
2023,
Номер
11(3)
Опубликована: Май 24, 2023
Amino
acids
play
a
crucial
role
in
the
growth
and
development
of
insects.
Aphids
cannot
ingest
enough
amino
plant
phloem
to
meet
their
requirements,
therefore,
they
are
mainly
dependent
on
obligate
symbiont
Buchnera
aphidicola
synthesize
essential
acids.
Besides
Buchnera,
aphids
may
harbor
another
facultative
symbiont,
Arsenophonus,
which
alters
requirement
cotton-melon
aphid
Aphis
gossypii
for
acid.
However,
it
is
unclear
how
Arsenophonus
regulates
requirement.
Here,
we
found
that
ameliorated
performance
A.
an
acid-deficient
diet.
A
deficiency
lysine
(Lys)
or
methionine
(Met)
led
changes
abundance
Arsenophonus.
suppressed
when
were
fed
normal
acid
diet,
but
this
suppression
was
eliminated
reversed
Lys-
Met-deficient
The
relative
positively
correlated
with
neither
them
body
weight
aphids.
expression
levels
Lys
Met
synthase
genes
affected
by
interaction
between
infections
abundance,
especially
reared
coexisted
bacteriocytes,
strengthens
interaction.
IMPORTANCE
can
In
study,
help
improve
aphids'
under
stress
changing
genes.
This
study
highlights
ameliorate
stress.
Molecular Ecology,
Год журнала:
2021,
Номер
30(15), С. 3882 - 3892
Опубликована: Май 26, 2021
Abstract
Organisms
harbour
myriad
microbes
which
can
be
parasitic
or
protective
against
harm.
The
costs
and
benefits
resulting
from
these
symbiotic
relationships
context‐dependent,
but
the
evolutionary
consequences
to
hosts
of
transitions
remain
unclear.
Here,
we
mapped
Leucobacter
genus
across
13,715
microbiome
samples
(163
studies)
reveal
a
global
distribution
as
free‐living
microbe
symbiont
animals
plants.
We
showed
that
geographically
distant
locations
(South
Africa,
France,
Cape
Verde),
isolates
vary
substantially
in
their
virulence
an
associated
animal
host,
Caenorhabditis
nematodes.
further
found
multiple
sequence
variants
co‐occur
wild
spp.
combined
with
natural
variation
provides
real‐world
potential
for
community
composition
influence
host
fitness.
examined
this
by
competing
C.
elegans
genotypes
differed
susceptibility
different
species
evolution
experiment.
One
was
host‐protective
another,
virulent
species.
tested
impact
genetic
background
on
patterns
host‐based
defence
evolution.
conferring
were
maintained
during
infection.
However,
when
protected
coinfection,
defences
nearly
lost
population.
Overall,
our
results
provide
insight
into
role
context
shaping
symbioses.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 21, 2024
Abstract
DPANN
is
a
widespread
and
highly
diverse
group
of
archaea
characterised
by
their
small
size,
reduced
genome,
limited
metabolic
pathways,
symbiotic
existence.
Known
species
are
predominantly
obligate
ectosymbionts
that
depend
on
host
for
survival
proliferation.
Despite
the
recent
expansion
in
this
clade,
structural
molecular
details
recognition,
host-DPANN
intercellular
communication,
adaptation
response
to
attachment
remain
unknown.
Here,
we
used
electron
cryotomography
(cryo-ET)
reveal
Candidatus
Micrarchaeota
(ARM-1)
interacts
with
its
host,
Metallosphaera
javensis
through
proteinaceous
nanotubes.
These
tubes
(∼4.5
nm
wide)
originate
extend
all
way
cytoplasm
act
like
tunnels
exchange.
Combining
cryo-ET
sub-tomogram
averaging,
revealed
situ
architectures
S-layers
structures
nanotubes
primed
extended
states,
providing
mechanistic
insights
into
substrate
Additionally,
performed
comparative
proteomics
genomic
analyses
identify
proteomic
changes
attachment.
Our
results
showed
striking
alterations
host-proteome
during
symbiosis
upregulation/downregulation
key
cellular
pathways.
Collectively,
these
provided
unprecedented
basis
communication
deepen
our
understanding
ectosymbiotic
relationships.
