Journal of Fungi,
Год журнала:
2023,
Номер
9(7), С. 725 - 725
Опубликована: Июль 4, 2023
The
vascular
plant
pathogenic
fungus
Verticillium
dahliae
has
to
adapt
environmental
changes
outside
and
inside
its
host.
V.
harbors
homologs
of
Neurospora
crassa
clock
genes.
molecular
functions
interactions
Frequency
(Frq)
Frq-interacting
RNA
helicase
(Frh)
in
controlling
conidia
or
microsclerotia
development
were
investigated
JR2.
Fungal
mutant
strains
carrying
gene
deletions,
an
FRH
point
mutation,
GFP
fusions
analyzed
on
transcript,
protein,
phenotypic
levels
as
well
pathogenicity
assays
tomato
plants.
Our
results
support
that
the
Frq–Frh
complex
is
formed
it
promotes
conidiation,
but
also
suppresses
therefore
delays
formation
response
light.
We
a
possible
link
between
negative
element
Frq
positive
regulator
Suppressor
flocculation
1
(Sfl1)
elucidate
regulatory
mechanism.
Both
Sfl1
are
mainly
present
during
onset
with
decreasing
protein
further
development.
Induction
requires
can
be
delayed
at
early
time
points
light
through
complex.
Gaining
knowledge
will
improve
control
fungal
growth
wilt
disease.
Journal of Fungi,
Год журнала:
2022,
Номер
8(6), С. 558 - 558
Опубликована: Май 25, 2022
FRQ
(frequency
protein),
FRH
(FRQ-interacting
RNA
helicase),
and
WC1
WC2
(white
collar
proteins)
are
major
clock
elements
that
govern
the
circadian
rhythm
in
Neurosporacrassa.
However,
deletion
of
frh
is
lethal
for
viability
N.
crassa,
making
it
elusive
whether
essential
or
nonessential
rhythm.
This
needs
clarification
a
fungus
where
not
lethal.
Here,
nuclear
ortholog
proved
Metarhiziumrobertsii.
The
nucleocytoplasmic
shuttling
M.
robertsii
FRQ,
WC1,
orthologs
was
light-dependent.
Yeast
two-hybrid
assay
validated
interactions
with
instead
WC2.
well,
shown
as
conidiation
rings
tint
dark
15
d-old
plate
cultures
grown
at
25
°C
light/dark
cycle
12:12,
abolished
absence
frq
wc1,
partially
disturbed
wc2,
but
unaffected
frh.
These
results
indicate
requirement
either
fungal
Further
analyses
mutants
revealed
dispensable
limited
roles
insect-pathogenic
lifecycle
robertsii,
respectively.
Journal of Fungi,
Год журнала:
2023,
Номер
9(7), С. 725 - 725
Опубликована: Июль 4, 2023
The
vascular
plant
pathogenic
fungus
Verticillium
dahliae
has
to
adapt
environmental
changes
outside
and
inside
its
host.
V.
harbors
homologs
of
Neurospora
crassa
clock
genes.
molecular
functions
interactions
Frequency
(Frq)
Frq-interacting
RNA
helicase
(Frh)
in
controlling
conidia
or
microsclerotia
development
were
investigated
JR2.
Fungal
mutant
strains
carrying
gene
deletions,
an
FRH
point
mutation,
GFP
fusions
analyzed
on
transcript,
protein,
phenotypic
levels
as
well
pathogenicity
assays
tomato
plants.
Our
results
support
that
the
Frq–Frh
complex
is
formed
it
promotes
conidiation,
but
also
suppresses
therefore
delays
formation
response
light.
We
a
possible
link
between
negative
element
Frq
positive
regulator
Suppressor
flocculation
1
(Sfl1)
elucidate
regulatory
mechanism.
Both
Sfl1
are
mainly
present
during
onset
with
decreasing
protein
further
development.
Induction
requires
can
be
delayed
at
early
time
points
light
through
complex.
Gaining
knowledge
will
improve
control
fungal
growth
wilt
disease.
