Bacteriophage Challenges in Industrial Processes: A Historical Unveiling and Future Outlook
Pathogens,
Год журнала:
2024,
Номер
13(2), С. 152 - 152
Опубликована: Фев. 7, 2024
Humans
have
used
fermentation
processes
since
the
Neolithic
period,
mainly
to
produce
beverages.
The
turning
point
occurred
in
1850s,
when
Louis
Pasteur
discovered
that
resulted
from
metabolism
of
living
microorganisms.
This
discovery
led
fast
development
fermented
food
production.
importance
industrial
based
on
significantly
increased.
Many
branches
industry
rely
metabolisms
bacteria,
for
example,
dairy
(cheese,
milk,
yogurts),
pharmaceutical
(insulin,
vaccines,
antibiotics),
or
production
chemicals
(acetone,
butanol,
acetic
acid).
These
are
mass
involving
a
large
financial
outlay.
That
is
why
it
essential
minimize
threats
One
major
threat
affecting
bacteria-based
bacteriophage
infections,
causing
substantial
economic
losses.
first
reported
phage
infections
appeared
1930s,
and
companies
still
struggle
fight
against
phages.
review
shows
cases
most
common
methods
prevent
infections.
Язык: Английский
Chitosan nano-formulation enhances stability and bactericidal activity of the lytic phage HK6
BMC Biotechnology,
Год журнала:
2025,
Номер
25(1)
Опубликована: Янв. 6, 2025
Abstract
Background
Successful
treatment
of
pathogenic
bacteria
like
Enterobacter
Cloacae
with
bacteriophage
(phage)
counteract
some
hindrance
such
as
phage
stability
and
immunological
clearance.
Our
research
is
focused
on
the
encapsulation
HK6
within
chitosan
nanoparticles.
Result
Encapsulation
significantly
improves
stability,
efficacy,
delivery
phages.
Chitosan
nanoparticles
(CS-NPs)
achieve
a
entrapment
efficiency
97%.
Fourier-transform
infrared
spectroscopy
(FT-IR)
reveals
shifts
towards
higher
wavenumbers
new
peak,
indicating
amide
bond
formation
successful
encapsulation.
The
average
particle
sizes
for
CS-NP
encapsulated
CS-NPs
were
180
±
10
nm
297
18
nm,
respectively.
Scanning
Electron
Microscopy
(SEM)
Transmission
(TEM)
analyses
reveal
that
are
larger
than
CS-NPs,
highlighting
Encapsulated
bacteriophages
maintain
its
effectiveness
at
pH
levels
11
12.
Both
free
thermostable
between
25
60
°C;
while
temperatures
(up
to
80
°C),
thermally
stable.
Over
four
days,
70.57%
phages
released
from
CS-NPs.
in
enhances
antibacterial
activity
first
2
h,
compared
or
alone.
Conclusion
This
suggests
exhibit
potentiality
biocontrol
agents
against
resistant
microorganisms
offering
an
alternative
Graphical
Язык: Английский
The activity of indigo carmine against bacteriophages: an edible antiphage agent
Applied Microbiology and Biotechnology,
Год журнала:
2025,
Номер
109(1)
Опубликована: Янв. 25, 2025
Abstract
Bacteriophage
infections
in
bacterial
cultures
pose
a
significant
challenge
to
industrial
bioprocesses,
necessitating
the
development
of
innovative
antiphage
solutions.
This
study
explores
potential
indigo
carmine
(IC),
common
FDA-approved
food
additive.
IC
demonstrated
selective
inactivation
DNA
phages
(P001,
T4,
T1,
T7,
λ)
with
EC
50
values
ranging
from
0.105
0.006
mg/mL
while
showing
no
activity
against
RNA
phage
MS2.
Fluorescence
correlation
spectroscopy
(FCS)
revealed
that
selectively
binds
dsDNA,
by
reduction
diffusion
coefficient,
whereas
binding
was
observed
ssDNA
or
RNA.
Mechanistically,
permeates
capsid,
leading
genome
ejection
and
capsid
deformation,
as
confirmed
TEM
imaging.
Under
optimal
conditions
(50
°C,
220
rpm),
achieved
up
7-log
titer,
kinetic
theory
supporting
enhanced
collision
frequency
induced
agitation.
Additionally,
protected
E.
coli
phage-induced
lysis
without
affecting
growth
protein
production,
GFP
expression
assays.
IC’s
effectiveness
environmental
safety,
combined
its
FDA
approval
cost-effectiveness,
make
it
promising
agent
for
applications.
Key
points
•
Indigo
effectively
inactivates
broad
spectrum
bacteriophages,
offering
protection
bacteria
cultures.
A
novel
application
food-grade,
environmentally
safe,
protecting
Antiphage
arises
carmine’s
interaction
within
harming
cells
compromising
production
Graphical
abstract
Язык: Английский
The effect of thyme essential oil and endothelial progenitor stem cells on lipopolysaccharide‐induced sepsis in C57BL/6 mice
Biotechnology and Applied Biochemistry,
Год журнала:
2024,
Номер
71(4), С. 835 - 848
Опубликована: Март 21, 2024
Abstract
Sepsis
is
a
potentially
fatal
syndrome
related
to
severe
systemic
inflammation
developed
by
infection.
Despite
different
antimicrobial
therapies,
morbidity
and
mortality
rates
remain
high.
Herbs
along
with
cell
therapy
have
been
introduced
as
promising
option
improve
the
symptoms
of
sepsis.
The
present
study
aimed
evaluate
therapeutic
effect
simultaneous
administration
thyme
essential
oil
(TEO)
endothelial
progenitor
stem
cells
(EPCs)
on
lipopolysaccharide
(LPS)‐induced
sepsis
in
C57BL/6
mice.
was
induced
C57Bl/6J
mice
intraperitoneal
injection
LPS,
followed
2
h
later
an
intravenous
EPCs
or
oral
TEO
EPCs.
After
10
days,
complete
blood
cell,
renal
liver
factors,
serum
levels
inflammatory
cytokines,
angiogenic
factors
were
measured.
Simultaneous
treatment
significantly
increased
survival
modulated
response
reducing
pro‐inflammatory
cytokines.
Moreover,
this
reduced
level
white
neutrophils
number
red
cells,
percentage
hematocrit,
hemoglobin.
combination
decreased
organ
injuries
assessed
lower
enzymes
alanine
aminotransferase
aspartate
compared
group.
Administration
also
improved
lung
function,
toll‐like
receptor
4
expression.
increase
LPS‐induced
model
acting
several
targets.
Thus,
can
be
feasible
approach
for
future
clinical
control
Язык: Английский
Copper Oxide Electrochemical Deposition to Create Antiviral and Antibacterial Nanocoatings
Langmuir,
Год журнала:
2024,
Номер
40(29), С. 14838 - 14846
Опубликована: Июль 9, 2024
The
impact
of
the
reaction
environment
on
formation
polycrystalline
layer
and
its
biomedical
(antimicrobial)
applications
were
analyzed
in
detail.
Copper
oxide
layers
synthesized
using
an
electrodeposition
technique,
with
varying
additives
influencing
morphology,
thickness,
chemical
composition.
Scanning
electron
microscopy
(SEM)
images
confirmed
successful
polyhedral
structures.
Unmodified
samples
(CuL)
crystallized
as
a
mixture
copper
(I)
(II),
thickness
approximately
1.74
μm.
inclusion
nonconductive
polymer
polyvinylpyrrolidone
(PVP)
during
synthesis
led
to
regular
compact
CuO-rich
structure
(CuL-PVP).
Conversely,
adding
glucose
resulted
forming
Cu
Язык: Английский
Real-time monitoring by interferometric light microscopy of phage suspensions for personalised phage therapy
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Дек. 30, 2024
Abstract
Phage
therapy
uses
viruses
(phages)
against
antibiotic
resistance.
Tailoring
treatments
to
specific
patient
strains
requires
stocks
of
various
highly
concentrated
purified
phages.
It,
therefore,
faces
challenges:
titration
duration
and
specificity
a
phage/bacteria
couple;
purification
affecting
stability;
suspensions
tending
aggregate.
To
address
these
challenges,
interferometric
light
microscopy
(ILM),
characterising
particles
(size,
concentration,
visual
homogeneity)
within
minutes,
was
applied
herein
anti-
Staphylococcus
aureus
myovirus
phage
suspensions.
Particle
concentration
linearly
correlated
with
infectious
titre
(R
2
>
0.97,
slope:
3
particles/plaque
forming
units
(PFU))
at
degrees
purification,
allowing
approximate
the
for
≥
×
10
8
PFU/mL,
thereby
encompassing
most
therapeutic
doses.
Purification
narrowed
homogenised
particle
distribution
while
maintaining
concentrations.
When
compared
dynamic
scattering,
electrophoretic
mobility,
UV/Visible-spectroscopy,
ILM
best
detected
aggregates
according
our
homemade
scoring.
Although
has
certain
limitations,
such
as
inability
detect
podoviruses
(hydrodynamic
diameter
<
80
nm),
or
measure
in
low-concentrated
(<
particles/mL),
present
proof-of-concept
positions
this
technique
valuable
quality
control
tool,
complement
rather
than
replacement
technique,
suspensions,
paving
way
further
investigations.
Язык: Английский