Microbiology Spectrum, Год журнала: 2023, Номер 11(2)
Опубликована: Фев. 28, 2023
Leptospirosis is a zoonotic disease of global importance. It caused by Gram-negative bacterial spirochete the genus Leptospira .
Язык: Английский
Computational and Structural Biotechnology Journal, Год журнала: 2021, Номер 19, С. 1806 - 1828
Опубликована: Янв. 1, 2021
Gram-negative bacteria harness multiple protein secretion systems and secrete a large proportion of the proteome. Proteins can be exported to periplasmic space, integrated into membrane, transported extracellular milieu, or translocated cytoplasm contacting cells. It is important for accurate, genome-wide annotation secreted proteins their pathways. In this review, we systematically classified according types in bacteria, summarized known features these proteins, reviewed algorithms tools prediction.
Язык: Английский
Процитировано
32
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Янв. 25, 2025
Bradyrhizobium sp. strain SUTN9-2 demonstrates cell enlargement, increased DNA content, and efficient nitrogen fixation in response to rice (Oryza sativa) extract. This is attributed the interaction between plant's cationic antimicrobial peptides (CAMPs) BacA-like transporter (BclA), similar bacteroid legume nodules. The present study reveals that can also establish functional endophytic interactions with chili (Capsicum annuum) tomato (Solanum lycopersicum) plants. When exposed extracts from tomato, exhibits elongation, polyploidy, reduced viability, effects being less pronounced for Transcriptomic cytological analyses revealed genes associated CAMP resistance, metabolism, fixation, defense responses, secretion systems were upregulated, while related cycle certain CAMP-resistance mechanisms downregulated, particularly suggests likely evolves resistance against CAMPs found rice, chili, plants through involving protease-chaperone DegP, AcrAB-TolC multidrug efflux pumps, polysaccharides. These facilitate efflux, degradation, formation of protective barriers resist CAMPs. Such adaptations enable persist colonize host despite pressures, influencing its differentiation, during various plant hosts.
Язык: Английский
Процитировано
0
Biomolecules, Год журнала: 2025, Номер 15(4), С. 459 - 459
Опубликована: Март 21, 2025
Type IV pili (T4P) machinery is critical for bacterial surface motility, protein secretion, and DNA uptake. This review highlights the ecological significance of T4P-dependent motility in Thermus thermophilus, a thermophilic bacterium isolated from hot springs. Unlike swimming T4P enables bacteria to move over two-dimensional surfaces through repeated cycles extension retraction pilus filaments. Notably, T. thermophilus exhibits upstream-directed migration under shear stress, known as rheotaxis, which appears represent an adaptive strategy unique thriving rapid water flows. Furthermore, contributes capture phages, indicating their multifunctionality natural environments. Understanding dynamics provides insights into survival evolution extreme habitats.
Язык: Английский
Процитировано
0
PLoS Pathogens, Год журнала: 2025, Номер 21(4), С. e1013008 - e1013008
Опубликована: Апрель 9, 2025
Many bacterial pathogens depend on a type II secretion (T2S) system to secrete virulence factors from the periplasm into extracellular milieu. T2S systems consist of an outer membrane secretin channel, periplasmic pseudopilus and inner membrane-associated assembly platform including cytoplasmic ATPase. The components are often conserved in different species, however, architecture is largely unknown. Here, we analysed predicted Xps-T2S plant-pathogenic bacterium Xanthomonas euvesicatoria . To facilitate these studies, generated modular xps- gene cluster by Golden Gate single promoter fragments. design allowed efficient deletion replacement genes insertion reporter fusions. Mutant approaches as well interaction crosslinking studies showed that XpsC, XpsL XpsM form trimeric complex which essential for associates with ATPase XpsE XpsD. Structural modeling revealed similar XpsCLM homologs Pseudomonas, Vibrio Klebsiella despite overall low amino acid sequence similarities. In X. euvesicatoria, fluorescence microscopy formation independent vice versa , suggesting dynamic process involves association preformed subcomplexes.
Язык: Английский
Процитировано
0
Journal of Biological Chemistry, Год журнала: 2020, Номер 296, С. 100147 - 100147
Опубликована: Дек. 4, 2020
Gram-negative pathogens are enveloped by an outer membrane that serves as a double-edged sword: On the one hand, it provides layer of protection for bacterium from environmental insults, including other bacteria and host immune system. restricts movement vital nutrients into cell plethora antigens can be detected systems. One strategy used to overcome these limitations is decoration surface gram-negative with proteins tethered through lipid anchor. These lipoproteins (SLPs) fulfill critical roles in evasion nutrient acquisition, but more bacterial genomes sequenced, we beginning discover their prevalence different mechanisms importantly how exploit them antimicrobial targets. This review will focus on representative SLPs use innate immunity, specifically areas nutritional immunity complement system evasion. We elaborate structures some notable required binding target molecules hosts this information alongside bioinformatics understand discovery new SLPs. foundation development therapeutics design vaccine antigens.
Язык: Английский
Процитировано
28
mBio, Год журнала: 2021, Номер 12(2)
Опубликована: Март 15, 2021
Protein secretion as well the assembly of bacterial motility appendages are central processes that substantially contribute to fitness and survival. This study highlights distinctive features mechanism serves these functions in cyanobacteria, which globally prevalent photosynthetic prokaryotes significantly primary production. Our studies biofilm development cyanobacterium
Язык: Английский
Процитировано
25
Methods in molecular biology, Год журнала: 2023, Номер unknown, С. 1 - 25
Опубликована: Ноя. 6, 2023
Язык: Английский
Процитировано
10
BMC Genomics, Год журнала: 2024, Номер 25(1)
Опубликована: Фев. 27, 2024
Abstract Background Shewanella xiamenensis , widely distributed in natural environments, has long been considered as opportunistic pathogen. Recently, significant changes the resistance spectrum have observed S. due to acquired antibiotic genes. Therefore, a pan-genome analysis was conducted illuminate genomic . Results Phylogenetic revealed three major clusters and singletons, among which close relationship between several strains discovered, regardless of their host niches. The “open” genomes with diversity accessory strain-specific took advantage towards environments. purifying selection pressure main force on genome evolution, especially conservative Only 53 gene families were under positive pressure. Phenotypic 21 classified multi-drug (MDR). Ten types genes two heavy metal operons discovered Mobile genetic elements horizontal transfer increased closely related MDR strains. carried variety virulence macromolecular secretion systems, indicating important roles pathogenicity adaptability. Type IV system 15 various sequence structures, it originated from different donors through transfer. Conclusions This study provided detailed insight into highlighting its capability acquire new mobile for adaptation environment human animals.
Язык: Английский
Процитировано
3
Molecular Microbiology, Год журнала: 2019, Номер 112(6), С. 1674 - 1683
Опубликована: Сен. 26, 2019
Summary In the diverse world of bacterial pili, type IV pili (Tfp) are unique for two reasons: their multifunctionality and ubiquity. This latter feature offers an extraordinary possibility, that is, to perform comparative studies in evolutionarily distant species order improve our fragmentary understanding Tfp biology. Regrettably, such potential has remained largely untapped, because, 20 years, have only been characterised diderm bacteria. However, recent monoderms started closing gap, revealing many interesting commonalities a few significant differences, extending frontiers knowledge Here, I review current state art field monoderm bacteria discuss resulting implications general assembly function these widespread filamentous nanomachines.
Язык: Английский
Процитировано
29
International Journal of Molecular Sciences, Год журнала: 2020, Номер 21(12), С. 4338 - 4338
Опубликована: Июнь 18, 2020
Marine bacteria display significant versatility in adaptation to variations the environment and stress conditions, including temperature shifts. Shewanella baltica plays a major role denitrification bioremediation marine environment, but is also identified be responsible for spoilage of ice-stored seafood. We aimed characterize transcriptional response S. cold order achieve better insight into mechanisms governing its adaptation. exposed bacterial cells 8 °C 90 180 min, assessed changes transcriptome with RNA sequencing validated RT-qPCR method. found that general associated massive downregulation gene expression, which covered about 70% differentially expressed genes. Enrichment analysis revealed upregulation only few pathways, aminoacyl-tRNA biosynthesis, sulfur metabolism flagellar assembly process. Downregulation was observed fatty acid degradation, amino secretion system. entire type II system transcriptionally shut down at low temperatures. reprogramming through induction RpoE repression RpoD sigma factors mediate response. Our study how diverse complex is.
Язык: Английский
Процитировано
24