Frontiers in Cellular and Infection Microbiology,
Год журнала:
2024,
Номер
14
Опубликована: Дек. 15, 2024
Ixodid
ticks
serve
as
hosts
and
transmission
vectors
for
several
obligate
intracellular
bacteria,
including
members
of
the
spotted
fever
group
(SFG)
Rickettsia
.
Although
generate
an
immune
response
to
bacterial
insults,
many
signaling
molecules
associated
with
how
they
may
contribute
vector
competence
are
undefined.
In
this
study,
we
isolated
a
full-length
dvrelish
transcript
from
Dermacentor
variabilis
,
which
encoded
Relish-type
NF-κB.
The
presence
canonical
Rel
homology
domain
(RHD)
consistent
NF-κB
proteins
suggested
role
in
tick
DvRelish.
expression
DvRelish
was
confirmed
tissues
fluorescent
microscopy
hemocytes
indicated
increased
following
infection
compared
non-tick-borne
pathogen.
To
further
determine
effect
dvRelish
gene
knockdown
on
rickettsial
infection,
used
RNA
interference-mediated
D.
demonstrated
that
transcription
decreased
after
24
h
post-injection
siRNA.
We
then
assessed
when
exposed
rickettsii
determined
inversely
loads
at
48
post-exposure.
Further
studies
required
broaden
understanding
differential
responses
SFG
elucidate
played
by
arthropod
system
competence.
Pathogens,
Год журнала:
2024,
Номер
13(7), С. 575 - 575
Опубликована: Июль 10, 2024
The
Rickettsia
species
transmitted
by
ticks
are
mostly
classified
within
the
spotted
fever
group
rickettsiae
(SFGR),
which
causes
tick-borne
rickettsiosis.
Although
efforts
have
been
made
to
investigate
their
prevalence
in
Republic
of
Korea
(ROK),
research
has
limited
certain
areas.
Furthermore,
pooling
method
for
does
not
fully
reflect
exact
infection
rate.
Therefore,
we
aimed
perform
molecular
identification
SFGR
elucidate
current
rickettsiosis
ROK.
was
identified
using
polymerase
chain
reaction
targeting
17
kDa
antigen,
ompA,
and
gltA,
followed
sequencing
phylogenetic
analysis.
In
total,
302
belonging
four
(Haemaphysalis
flava,
H.
longicornis,
Ixodes
nipponensis,
Amblyomma
testudinarium)
were
collected
between
April
November
2022.
overall
rate
26.8%
(81/302
patients).
Both
adult
nymphal
increased
during
April-May,
reaching
peaks
June,
a
marked
decline
August
July,
respectively.
Phylogenetic
analysis
revealed
three
(R.
monacensis,
R.
heilongjiangensis,
Candidatus
jingxinensis)
SFGR.
Thus,
our
results
emphasize
importance
tick
surveys
prevention
management
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Окт. 8, 2024
Background
Microbial
communities
significantly
influence
the
vector
capacity
of
ticks,
which,
along
with
tick-borne
diseases,
pose
an
increasing
global
threat.
Due
to
substantial
individual
variability
caused
by
various
factors,
it
is
essential
assess
tick
microbial
and
vectorial
capacities
under
different
environmental
conditions.
However,
there
a
relative
scarcity
research
on
pathogen
transmission
ticks
in
physiological
states
conditions,
especially
Hainan
Island,
southern
China.
Methods
From
2021
2022,
we
collected
4,167
samples,
grouping
them
blood
meal
status,
developmental
stage,
sex,
time,
geographical
location,
species.
We
selected
128
samples
for
full-length
16S
rRNA
sequencing
describe
community
characteristics
identify
potential
biomarkers.
Seven
hundred
seventy-two
were
tested
seven
pathogens
(
Rickettsia
,
Borrelia
burgdorferi
Ehrlichia
Anaplasma
Theileria
Babesia
Hepatozoon
),
sera
from
208
residents
Island
IgG
antibodies
against
B.
.
Results
Blood
species
influenced
ticks.
observed
distinct
across
states.
noted
non-random
replacement
stable
transient
species,
functional
differences
between
parasitic
engorged
mainly
driven
Functionally,
three
response
patterns:
both
together
six
factors.
identified
273
biomarkers
(200
robust
core
73
differential
species).
Six
genera
eight
detected
overall
positivity
rate
12.44%
(96/772).
Among
humans,
18.27%
(38/208)
serum
positive
at
least
one
IgG.
Conclusion
Our
findings
indicate
that
these
factors
capacity,
varying
effects
competence
inconsistent
impacts
This
study
supplemented
understanding
assessed
relatively
high
risk
region,
evaluated
impact
capacity.
Tropical Medicine and Infectious Disease,
Год журнала:
2024,
Номер
9(11), С. 254 - 254
Опубликована: Окт. 24, 2024
Ticks
are
vectors
of
public
health
concern
because
the
pathogens
they
transmit
can
cause
detrimental
diseases
in
humans.
Lyme
disease,
tick-borne
relapsing
fever,
human
granulocytic
anaplasmosis,
Rocky
Mountain
spotted
encephalitis,
Crimean-Congo
hemorrhagic
and
babesiosis
some
most
common
caused
by
transmitted
ticks.
The
overlap
between
activities
tick
habitats
is
growing,
contributing
to
an
increase
disease
cases.
Unfortunately,
pregnancy
as
a
risk
factor
for
largely
ignored.
In
this
narrative
review
we
use
case
reports,
epidemiological
studies,
animal
studies
evaluate
maternal,
pregnancy,
fetal
outcomes
during
pregnancy.
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2024,
Номер
14
Опубликована: Дек. 15, 2024
Ixodid
ticks
serve
as
hosts
and
transmission
vectors
for
several
obligate
intracellular
bacteria,
including
members
of
the
spotted
fever
group
(SFG)
Rickettsia
.
Although
generate
an
immune
response
to
bacterial
insults,
many
signaling
molecules
associated
with
how
they
may
contribute
vector
competence
are
undefined.
In
this
study,
we
isolated
a
full-length
dvrelish
transcript
from
Dermacentor
variabilis
,
which
encoded
Relish-type
NF-κB.
The
presence
canonical
Rel
homology
domain
(RHD)
consistent
NF-κB
proteins
suggested
role
in
tick
DvRelish.
expression
DvRelish
was
confirmed
tissues
fluorescent
microscopy
hemocytes
indicated
increased
following
infection
compared
non-tick-borne
pathogen.
To
further
determine
effect
dvRelish
gene
knockdown
on
rickettsial
infection,
used
RNA
interference-mediated
D.
demonstrated
that
transcription
decreased
after
24
h
post-injection
siRNA.
We
then
assessed
when
exposed
rickettsii
determined
inversely
loads
at
48
post-exposure.
Further
studies
required
broaden
understanding
differential
responses
SFG
elucidate
played
by
arthropod
system
competence.
