Modulation of host ATP levels by secreted bacterial effectors DOI Creative Commons

Chunlin He,

Chuang Li, Yao Liu

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Май 20, 2025

Adenosine 5'-triphosphate (ATP) is the currency of energy in cells; it plays essential roles virtually all cellular processes, ranging from basic metabolism to signaling development and disease. The opportunistic bacterial pathogen Legionella pneumophila utilizes Dot/Icm type IV secretion system deliver over 300 effectors into host cells, some which utilize ATP perform biochemical reactions catalyzed by their unique enzymatic activities. However, whether L. directly regulates level cells unknown. Here, we discover that substrate Ceg14 (Lpg0437, a.k.a. SidL) an ATP/dATPase, after being activated protein actin, efficiently converts dATP adenosine deoxyadenosine monophosphate, respectively a mechanism requires its S-HxxxE (x, any amino acid) motif. activity regulated metaeffector AnkJ (Lpg0436, LegA11), inhibits ATPase via direct protein-protein interactions. impose temporal regulation levels pneumophila-infected cells. Our results demonstrate modulates create environment permissive for growth.

Язык: Английский

Modulation of host ATP levels by secreted bacterial effectors DOI Creative Commons

Chunlin He,

Chuang Li, Yao Liu

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Май 20, 2025

Adenosine 5'-triphosphate (ATP) is the currency of energy in cells; it plays essential roles virtually all cellular processes, ranging from basic metabolism to signaling development and disease. The opportunistic bacterial pathogen Legionella pneumophila utilizes Dot/Icm type IV secretion system deliver over 300 effectors into host cells, some which utilize ATP perform biochemical reactions catalyzed by their unique enzymatic activities. However, whether L. directly regulates level cells unknown. Here, we discover that substrate Ceg14 (Lpg0437, a.k.a. SidL) an ATP/dATPase, after being activated protein actin, efficiently converts dATP adenosine deoxyadenosine monophosphate, respectively a mechanism requires its S-HxxxE (x, any amino acid) motif. activity regulated metaeffector AnkJ (Lpg0436, LegA11), inhibits ATPase via direct protein-protein interactions. impose temporal regulation levels pneumophila-infected cells. Our results demonstrate modulates create environment permissive for growth.

Язык: Английский

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