Rho-dependent termination: a bacterial evolutionary capacitor for stress resistance

Transcription, Год журнала: 2025, Номер unknown, С. 1 - 14

Опубликована: Март 5, 2025

Since the Modern Synthesis, interest has grown in resolving "black box" between genotype and phenotype. Contained within this black box are highly plastic RNA proteins with global effects on chromosome integrity gene expression that serve as evolutionary capacitors - elements enable accumulation buffering of genetic variation normal conditions reveal hidden when induced by environmental stress. Discussion primarily focused eukaryotic translation factors chaperones, such Hsp90 PSI+ prion. However, due to coupling transcription prokaryotes, can be equally impactful modulation phenotypes. In review, we discuss prokaryotic terminator Rho how mutagenesis plasticity influence epistasis, evolvability, adaptation stress bacteria. We generated nature, laboratory mutagenesis, experimental evolution; is constrained or encouraged Rho's extensive network protein interactors. Exploring role an capacitor, along identifying additional function, significantly advance our understanding organisms adapt thrive diverse environments.

Язык: Английский

Evolution of pH-sensitive transcription termination in Escherichia coli during adaptation to repeated long-term starvation

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(39)

Опубликована: Сен. 19, 2024

Fluctuating environments that consist of regular cycles co-occurring stress are a common challenge faced by cellular populations. For population to thrive in constantly changing conditions, an ability coordinate rapid response is essential. Here, we identify mutation conferring arginine-to-histidine (Arg His) substitution the transcription terminator Rho. The rho R109H frequently arose Escherichia coli populations experimentally evolved under repeated long-term starvation during which accumulation metabolic waste followed transfer into fresh media results drastic environmental pH fluctuations associated with feast and famine. Metagenomic sequencing revealed containing also possess putative loss-of-function mutations ydcI , encodes recently characterized factor homeostasis. Genetic reconstructions these show allele confers plasticity via alkaline-induced reduction Rho function that, when found tandem Δ allele, leads intracellular alkalization genetic assimilation mutant function. We further Arg His substitutions at analogous sites alleles from species regularly experience neutral alkaline their environments. Our suggest may serve rapidly complex physiological responses through sensing shed light on how use cues complex, fluctuating

Язык: Английский

Effects of Environmental Stresses on Synthesis of 2-Phenylethanol and IAA by Enterobacter sp. CGMCC 5087

Microorganisms, Год журнала: 2024, Номер 12(4), С. 663 - 663

Опубликована: Март 26, 2024

2-Phenylethanol (2-PE) and indole-3-acetic acid (IAA) are important secondary metabolites produced by microorganisms, their production closely linked to the growth state of microorganisms environmental factors. Enterobacter CGMCC 5087 can produce both 2-PE IAA depending on α-ketoacid decarboxylase KDC4427. This study aimed investigate effects different environment factors including osmotic pressure, temperature, pH synthesis in sp. 5087. The bacteria exhibited an enhanced capacity for while not affecting under 5% NaCl 4.5 stress conditions. In with 9.5, remained unchanged decreased. ability was increase temperature within range 25 °C 37 °C, affected significantly. Additionally, expression KDC4427 varied Under decreased gene increased. However, altering did result significant differences levels, elevated caused a decrease expression. Furthermore, molecular docking dynamics simulations suggested that these conditions may induce fluctuation geometry shape binding cavity, energy, especially dαC-C- value, which played key roles enzyme activity. These results provide insights strategies metabolic products bacterial fermentation, even unfavorable

Язык: Английский