An Infection-Relevant Transcriptomic Compendium for Salmonella enterica Serovar Typhimurium

Cell Host & Microbe, Год журнала: 2013, Номер 14(6), С. 683 - 695

Опубликована: Дек. 1, 2013

Bacterial transcriptional networks consist of hundreds transcription factors and thousands promoters. However, the true complexity in a bacterial pathogen effect environments encountered during infection remain to be established. We present simplified approach for global promoter identification bacteria using RNA-seq-based transcriptomic analyses 22 distinct infection-relevant environmental conditions. Individual RNA samples were combined identify most 3,838 Salmonella enterica serovar Typhimurium promoters just two RNA-seq runs. vitro conditions stimulated characteristic signatures, suite induced 86% all S. genes. highlight that induce pathogenicity islands small expression landscape 280 sRNAs. This publicly available compendium environmentally controlled every feature constitutes useful resource research community.

Язык: Английский

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Magnesium Oxide Nanoparticles: Effective Agricultural Antibacterial Agent Against Ralstonia solanacearum

Frontiers in Microbiology, Год журнала: 2018, Номер 9

Опубликована: Апрель 25, 2018

Magnesium (Mg) is an essential mineral element for plants and nontoxic to organisms. In this study, we took advantage of nanotechnologies systematically investigate the antibacterial mechanisms magnesium oxide nanoparticles (MgONPs) against phytopathogen Ralstonia solanacearum (R. solanacearum) in vitro vivo first time. R. has contributed catastrophic bacterial wilt, which resulted world-wide reduction tobacco production. The results demonstrated that MgONPs possessed statistically significant concentration-dependent activity, minimum inhibitory concentration (MIC) bactericidal (MBC) were measured as 200 250 μg/mL, respectively. Additional studies, aimed at understanding toxicity mechanism MgONPs, indicated physical injury occurred cell membranes, along with decreased motility biofilm formation ability solanacearum, due direct attachment surfaces cells, was observed by scanning electron microscopy (SEM) transmission (TEM). Reactive oxygen species (ROS) accumulation could also be important reason action, inducing DNA damage. assessment assay under greenhouse conditions had exerted a large effect on reducing wilt index. Altogether, suggest development alternative agents will become new research subject.

Язык: Английский

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Toxicity of Nanomaterials: Exposure, Pathways, Assessment, and Recent Advances

ACS Biomaterials Science & Engineering, Год журнала: 2018, Номер 4(7), С. 2237 - 2275

Опубликована: Июнь 8, 2018

Growth in production of manufactured goods and the use nanomaterials consumer products has mounted past few decades. Nanotoxicology or toxicity assessment these engineered is required to understand possible adverse effects their fate inside human body. The present review a one stop intended be state art understanding on nanotoxicity. It provides summation various kinds cell death also discusses different types toxicities along with studies. physiological impact imparted cells (reactive oxygen species generation resultant oxidative stress, inflammation, other nonoxidant pathways). Moreover, it physicochemical properties (size, morphology, surface charge, coating) governing cytotoxicity properties. details major pathways nanomaterial uptake outcome. Additionally, methods for exposure (skin, respiratory tract, gastrointestinal blood brain barrier, liver, spleen). Furthermore, an entire new section contributed discussion all assays (cytotoxicity, proliferation, genotoxicity assays). A summarized recent advances vitro, silico, vivo studies (metal, metal oxides, carbon nanotubes, graphene, novel materials) made. brief account safety guidelines handling nanomaterials. Finally, uses commercial are discussed detail.

Язык: Английский

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Lag Phase Is a Dynamic, Organized, Adaptive, and Evolvable Period That Prepares Bacteria for Cell Division

Journal of Bacteriology, Год журнала: 2019, Номер 201(7)

Опубликована: Янв. 15, 2019

Lag is a temporary period of nonreplication seen in bacteria that are introduced to new media. Despite latency being described by Müller 1895, only recently have we gained insights into the cellular processes characterizing lag phase. This review covers literature date on transcriptomic, proteomic, metabolomic, physiological, biochemical, and evolutionary features prokaryotic lag. Though commonly as preparative phase allows harvest nutrients adapt environments, implications recent studies indicate refinement this view well deserved. As shown, dynamic, organized, adaptive, evolvable process protects from threats, promotes reproductive fitness, broadly relevant study bacterial evolution, host-pathogen interactions, antibiotic tolerance, environmental biology, molecular microbiology, food safety.

Язык: Английский

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Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression

Microbial Cell Factories, Год журнала: 2014, Номер 13(1)

Опубликована: Ноя. 11, 2014

Because of its adaptability to sites polluted with toxic chemicals, the model soil bacterium Pseudomonas putida is naturally endowed a number metabolic and stress-endurance qualities which have considerable value for hosting energy-demanding redox reactions thereof. The growing body knowledge on P. strain KT2440 has been exploited rational design derivative in genome heavily edited order construct robust microbial cell factory.Eleven non-adjacent genomic deletions, span 300 genes (i.e., 4.3% entire genome), were eliminated; thereby enhancing desirable traits eliminating attributes are detrimental an expression host. Since ATP NAD(P)H availability - as well genetic instability, generally considered be major bottlenecks performance platform strains, suite functions that drain high-energy phosphate from cells and/or consume targeted particular, whole flagellar machinery. Four prophages, two transposons, three components DNA restriction-modification systems eliminated well. resulting (P. EM383) displayed growth properties lag times, biomass yield, specific rates) clearly superior precursor wild-type KT2440. Furthermore, it tolerated endogenous oxidative stress, acquired replicated exogenous DNA, survived better stationary phase. bi-cistronic GFP-LuxCDABE reporter system proxy combined vitality, revealed deletions EM383 brought about increase >50% overall physiological vigour.The rationally modified allowed functional implanted by directly improving currency sustains gene flow, instead resorting classical approaches (e.g., increasing promoter strength constructs interest).

Язык: Английский

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SuperSegger: robust image segmentation, analysis and lineage tracking of bacterial cells

Molecular Microbiology, Год журнала: 2016, Номер 102(4), С. 690 - 700

Опубликована: Авг. 29, 2016

Summary Many quantitative cell biology questions require fast yet reliable automated image segmentation to identify and link cells from frame‐to‐frame, characterize the morphology fluorescence. We present SuperSegger , an MATLAB‐based processing package well‐suited analysis of high‐throughput live‐cell fluorescence microscopy bacterial cells. incorporates machine‐learning algorithms optimize cellular boundaries error resolution reliably frame‐to‐frame. Unlike existing packages, it can segment microcolonies with many cells, facilitating cell‐cycle dynamics in bacteria as well cell‐contact mediated phenomena. This has a range built‐in capabilities for characterizing including identification division events, mother, daughter neighbouring computing statistics on fluorescence, location intensity fluorescent foci. provides variety postprocessing data visualization tools single population level analysis, such histograms, kymographs, frame mosaics, movies consensus images. Finally, we demonstrate power by analyzing lag phase growth resolution.

Язык: Английский

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Valorisation of potato peel wastes for bioethanol production using simultaneous saccharification and fermentation: Process optimization and kinetic assessment

Renewable Energy, Год журнала: 2019, Номер 146, С. 1031 - 1040

Опубликована: Июль 8, 2019

Язык: Английский

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Microbial Metabolic Capacity for Intestinal Folate Production and Modulation of Host Folate Receptors

Frontiers in Microbiology, Год журнала: 2019, Номер 10

Опубликована: Окт. 9, 2019

Microbial metabolites, including B complex vitamins contribute to diverse aspects of human health. Folate, or vitamin

Язык: Английский

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Applications of Fourier Transform-Infrared spectroscopy in microbial cell biology and environmental microbiology: advances, challenges, and future perspectives

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Ноя. 21, 2023

Microorganisms play pivotal roles in shaping ecosystems and biogeochemical cycles. Their intricate interactions involve complex biochemical processes. Fourier Transform-Infrared (FT-IR) spectroscopy is a powerful tool for monitoring these interactions, revealing microorganism composition responses to the environment. This review explores diversity of applications FT-IR within field microbiology, highlighting its specific utility microbial cell biology environmental microbiology. It emphasizes key such as identification, process monitoring, wall analysis, biofilm examination, stress response assessment, interaction investigation, showcasing crucial role advancing our understanding systems. Furthermore, we address challenges including sample complexity, data interpretation nuances, need integration with complementary techniques. Future prospects microbiology include wide range transformative advancements. These development comprehensive standardized libraries precise advanced analytical techniques, adoption high-throughput single-cell real-time using portable systems incorporation into ecological modeling predictive insights changes. innovative avenues promise significantly advance microorganisms their various ecosystems.

Язык: Английский

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In vitro influence of PEG functionalized ZnO–CuO nanocomposites on bacterial growth

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Янв. 14, 2024

Abstract Polyethyleneglycol-coated biocompatible CuO–ZnO nanocomposites were fabricated hydrothermally varying Zn:Cu ratios as 1:1, 2:1, and 1:2, their antibacterial activity was determined through the well diffusion method against Gram-negative Escherichia coli , Pseudomonas aeruginosa Klebsiella pneumoniae, Gram-positive Staphylococcus aureus. The minimum inhibitory concentration bactericidal values of synthesized samples determined. Subsequently, time synergy kill assay performed to elucidate nature overall effect aforementioned bacterial species. mean zone inhibition for all four are presented. increased with increasing nanocomposite (20, 40 60 mg/ml) on species except S. aureus . According MBC/MIC ratio, ZnO found be bacteriostatic E. P. aeruginosa, K. pneumoniae 2:1 A observed in presence 1:1 whereas, it showed a pneumoniae. 1:2 exhibited while coli, metal oxide more sensitive towards strain than strains Further, possess anti-oxidant shown by DPPH assay.

Язык: Английский

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