International Journal of Biological Macromolecules, Год журнала: 2024, Номер unknown, С. 136978 - 136978
Опубликована: Окт. 1, 2024
Язык: Английский
Advanced Science, Год журнала: 2024, Номер 11(30)
Опубликована: Июнь 14, 2024
Abstract As “non‐cellular organisms”, viruses need to infect living cells survive themselves. The virus infection must alter host's metabolisms. However, the influence of metabolites from altered metabolisms virus‐infected host on virus‐host interactions remains largely unclear. To address this issue, shrimp, a representative species crustaceans, is challenged with white spot syndrome (WSSV) in study. vivo results presented that WSSV enhanced shrimp glycolysis, leading accumulation lactate. lactate turn promoted site‐specific histone lactylation (H3K18la and H4K12la) p300/HDAC1/HDAC3‐dependent manner. H3K18la H4K12la are enriched promoters 75 target genes, which modification upregulated expression ribosomal protein S6 kinases 2 ( S6K2 ) hosts promote infection. Further data revealed virus‐encoded miR‐N20 targeted hypoxia inducible factor‐1α HIF‐1α inhibit suppression H4K12la. Therefore, findings contributed novel insights into effects underlying mechanism virus‐induced interactions, providing new targets for control
Язык: Английский
Процитировано
12
PLoS Pathogens, Год журнала: 2025, Номер 21(1), С. e1012872 - e1012872
Опубликована: Янв. 13, 2025
Mitochondria, recognized as the "powerhouse" of cells, play a vital role in generating cellular energy through dynamic processes such fission and fusion. Viruses have evolved mechanisms to hijack mitochondrial function for their survival proliferation. Here, we report that infection with swine arterivirus porcine reproductive respiratory syndrome virus (PRRSV), manipulates mitochondria calcium ions (Ca2+) induce mitophagy, thereby reprogramming metabolism facilitate its own replication. Mechanistically, PRRSV-induced is caused by elevated levels Ca2+, derived from endoplasmic reticulum (ER) inositol 1,4,5-triphosphate receptor (IP3R)-voltage-dependent anion channel 1 (VDAC1)-mitochondrial uniporter (MCU) channels. This process associated increased mitochondria-associated membranes (MAMs), mediated upregulated expression sigma non-opioid intracellular (SIGMAR1). Elevated Ca2+ further activates Ca2+/CaM-dependent protein kinase β (CaMKKβ)-AMP-activated (AMPK)-dynamin-related (DRP1) signaling pathway, which interacts (FIS1) dynamics proteins 49 kDa (MiD49) promote fission. PRRSV infection, alongside fission, triggers mitophagy via PTEN-induced putative (PINK1)-Parkin RBR E3 ubiquitin (Parkin) promoting glycolysis excessive lactate production study reveals mechanism regulates during providing new insights into interplay between host cell metabolism.
Язык: Английский
Процитировано
2
EMBO Reports, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 4, 2024
Язык: Английский
Процитировано
6
Journal of Advanced Research, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 1, 2024
Lactate was once considered as metabolic waste for a long time. In 2019, Professor Zhao Yingming's team from the University of Chicago found that lactate could also be used substrate to induce histone lactylation and regulate gene expression. Since then, researchers have discovered lactate-mediated play important regulatory roles in various physiological pathological processes.
Язык: Английский
Процитировано
4
Vaccines, Год журнала: 2025, Номер 13(1), С. 46 - 46
Опубликована: Янв. 8, 2025
As an essential type of vaccine, live attenuated vaccines (LAVs) play a crucial role in animal disease prevention and control. Nevertheless, developing LAVs faces the challenge balancing safety efficacy. Understanding mechanisms viruses use to antagonize host antiviral innate immunity may help precisely regulate vaccine strains maintain strong immunogenicity while reducing their pathogenicity. It improve efficacy LAVs, as well provide more reliable means for control infectious livestock diseases. Therefore, exploring viral antagonistic is significant clue which helps explore virulence factors (as new targets) provides vital theoretical basis technical support development. Among viruses, ASFV, PRRSV, PRV, CSFV, FMDV, PCV, PPV, AIV are some typical representatives. conduct in-depth research summarize strategies these viruses. Studies have indicated that by directly or indirectly blocking signaling pathways. In addition, also do this antagonizing restriction targeting replication cycle. Beyond that, via regulating apoptosis, metabolic pathways, stress granule formation. A summary might understanding pathogenic mechanism based on factors.
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 139973 - 139973
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Viruses, Год журнала: 2025, Номер 17(2), С. 167 - 167
Опубликована: Янв. 24, 2025
Porcine reproductive and respiratory syndrome virus (PRRSV) African swine fever (ASFV) cause serious economic losses to the industry worldwide. Both viruses show a tropism for macrophages, based on use of specific entry mediators (e.g., Siglec-1 CD163). Identifying additional viral is essential advancing antiviral vaccine development. In this context, monoclonal antibodies (mAbs) are valuable tools. This study employed library 166 mAbs targeting porcine alveolar macrophages (PAMs) identify candidates capable blocking early infection stages, including binding, internalization, fusion. Immunofluorescence analysis revealed 74 with cytoplasmic staining 70 membrane staining. Fifteen reacted blood monocytes as determined by flow cytometry. mAb assays were performed at 4 °C 37 analyze ability block PRRSV and/or ASFV infections in PAMs. The 28C10 significantly blocked (96% 80% °C) (64% 81% infections. 28G10B6 (86% 74% partially (35% 64% 26B8F5-I only (65% 46% °C). Western blotting mass spectrometry identified corresponding proteins (28C10; 250 kDa), MYH9 (28G10B6; 260 ANXA1 (26B8F5-I; kDa). Our findings indicative that Siglec-1, MYH9, play role PRRSV/ASFV into macrophages.
Язык: Английский
Процитировано
0
Virology, Год журнала: 2025, Номер 605, С. 110466 - 110466
Опубликована: Фев. 25, 2025
Язык: Английский
Процитировано
0
Aquaculture, Год журнала: 2025, Номер unknown, С. 742390 - 742390
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Frontiers in Pharmacology, Год журнала: 2025, Номер 16
Опубликована: Март 17, 2025
Lung cancer, one of the most lethal malignancies, has seen its therapeutic strategies become a focal point significant scientific attention. Intrinsic immune signaling pathways play crucial roles in anti-tumor immunity but face clinical application challenges despite promising preclinical outcomes. Lactylation, an emerging research focus, may influences lung cancer progression by modulating functions histones and non-histone proteins. Recent findings have suggested that lactylation regulates key intrinsic molecules, including cGAS-STING, TLR, RIG-I, thereby impacting interferon expression. However, precise mechanisms which governs remain unclear. This review presents comprehensive systematic analysis relationship between emphasizes innovative perspective linking lactylation-mediated epigenetic modifications with regulation. By thoroughly examining current findings, this uncovers potential regulatory highlights implications targeting cancer. Future investigations into intricate interactions are anticipated to unveil novel targets strategies, potentially improving patient survival
Язык: Английский
Процитировано
0