Interactions between pili affect the outcome of bacterial competition driven by the type VI secretion system DOI Creative Commons
Simon B. Otto, Richard Servajean, Alexandre Lemopoulos

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 26, 2023

Abstract The bacterial type VI secretion system (T6SS) is a widespread, kin-discriminatory weapon capable of shaping microbial communities. Due to the system’s dependency on contact, cellular interactions can lead either competition or kin protection. Cell-to-cell contact often accomplished via surface-exposed IV pili (T4P). In Vibrio cholerae, these T4P facilitate specific when bacteria colonize natural chitinous surfaces. However, it has remained unclear whether and, if so, how affect bacterium’s T6SS-mediated killing. this study, we demonstrate that pilus-mediated be harnessed reduce population V. cholerae under liquid growth conditions in T6SS-dependent manner. We also show naturally occurring diversity determines likelihood cell-to-cell consequently, extent competition. To determine factors enable hinder T6SS’s targeted reduction competitors carrying pili, developed physics-grounded computational model for autoaggregation. Collectively, our research demonstrates involved impose selective burden encounters non-kin cells possess an active T6SS. Additionally, study underscores significance protecting closely related individuals from T6SS attacks through autoaggregation and spatial segregation.

Язык: Английский

Interactions between pili affect the outcome of bacterial competition driven by the type VI secretion system DOI Creative Commons
Simon B. Otto, Richard Servajean,

Alexandre Lemopoulos

и другие.

Current Biology, Год журнала: 2024, Номер 34(11), С. 2403 - 2417.e9

Опубликована: Май 14, 2024

The bacterial type VI secretion system (T6SS) is a widespread, kin-discriminatory weapon capable of shaping microbial communities. Due to the system's dependency on contact, cellular interactions can lead either competition or kin protection. Cell-to-cell contact often accomplished via surface-exposed IV pili (T4Ps). In Vibrio cholerae, these T4Ps facilitate specific when bacteria colonize natural chitinous surfaces. However, it has remained unclear whether and, if so, how affect bacterium's T6SS-mediated killing. this study, we demonstrate that pilus-mediated be harnessed by T6SS-equipped V. cholerae kill non-kin cells under liquid growth conditions. We also show naturally occurring diversity determines likelihood cell-to-cell consequently, extent competition. To determine factors enable hinder T6SS's targeted reduction competitors carrying pili, developed physics-grounded computational model for autoaggregation. Collectively, our research demonstrates involved in impose selective burden encounters possess an active T6SS. Additionally, study underscores significance T4P protecting closely related individuals from T6SS attacks through autoaggregation and spatial segregation.

Язык: Английский

Процитировано

5
Constitutive expression of the Type VI Secretion System carries no measurable fitness cost in Vibrio cholerae DOI Creative Commons
Christopher Zhang, Sayantan Datta, William C. Ratcliff

и другие.

Ecology and Evolution, Год журнала: 2024, Номер 14(3)

Опубликована: Март 1, 2024

Abstract The Type VI Secretion System (T6SS) is a widespread and highly effective mechanism of microbial warfare; it confers the ability to efficiently kill susceptible cells within close proximity. Due its large physical size, complexity, ballistic basis for intoxication, has widely been assumed incur significant growth costs in absence improved competitive outcomes. In this study, we precisely examine fitness constitutive T6SS firing bacterium Vibrio cholerae . We find that, contrary expectations, expression negligible impact on growth, reducing by 0.025 ± 0.5% (95% CI) relative T6SS− control. Mathematical modeling populations demonstrates due clonal interference, will often be neutral, with little evolutionary Our findings underscore importance measuring social behaviors help explain prevalence across Gram‐negative bacteria.

Язык: Английский

Процитировано

3
Quorum-sensing- and type VI secretion-mediated spatiotemporal cell death drives genetic diversity in Vibrio cholerae DOI Creative Commons
Ameya A. Mashruwala, Boyang Qin, Bonnie L. Bassler

и другие.

Cell, Год журнала: 2022, Номер 185(21), С. 3966 - 3979.e13

Опубликована: Сен. 26, 2022

Bacterial colonies composed of genetically identical individuals can diversify to yield variant cells with distinct genotypes. Variant outgrowth manifests as sectors. Here, we show that Type VI secretion system (T6SS)-driven cell death in Vibrio cholerae imposes a selective pressure for the emergence strains evade T6SS-mediated killing. occurs two spatiotemporal phases, and each phase is driven by particular T6SS toxin. The first regulated quorum sensing drives sectoring. second does not require T6SS-injection machinery. V. isolated from colony sectors encode mutated quorum-sensing components confer growth advantages suppressing T6SS-killing activity while simultaneously boosting defenses. Our findings eliminate sibling cells, suggesting role intra-specific antagonism. We propose quorum-sensing-controlled T6SS-driven killing promotes genetic diversity, including natural habitats during disease.

Язык: Английский

Процитировано

11
Quorum sensing orchestrates parallel cell death pathways in Vibrio cholerae via Type 6 secretion-dependent and -independent mechanisms DOI Creative Commons
Ameya A. Mashruwala, Bonnie L. Bassler

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(46)

Опубликована: Ноя. 5, 2024

Quorum sensing (QS) is a cell-to-cell communication process that enables bacteria to coordinate group behaviors. In Vibrio cholerae colonies, program of spatial-temporal cell death among the QS-controlled traits. Cell occurs in two phases, first along colony rim, and subsequently, at center. Both phases are driven by type 6 secretion system (T6SS). Here, we show HapR, master QS regulator, does not control t6ss gene expression nor T6SS-mediated killing activity. Nonetheless, Δ hapR strain displays no rim. RNA-Sequencing (RNA-Seq) analyses reveal HapR activates an operon containing four genes unknown function, vca0646-0649. Epistasis overexpression studies genes, vca0646 vca0647 , required drive both strain. Thus, - 0649 regulated but act independently T6SS machinery cause death, suggesting second, parallel pathway exists V. .

Язык: Английский

Процитировано

2
Quorum sensing orchestrates parallel cell death pathways inVibrio choleraevia Type 6 secretion dependent and independent mechanisms DOI Creative Commons
Ameya A. Mashruwala, Bonnie L. Bassler

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 23, 2024

Quorum sensing (QS) is a cell-to-cell communication process that enables bacteria to coordinate group behaviors. In

Язык: Английский

Процитировано

1
Sporadic type VI secretion in seventh pandemic Vibrio cholerae DOI
Alexis Proutière, Natália C. Drebes Dörr,

Loriane Bader

и другие.

Microbiology, Год журнала: 2023, Номер 169(5)

Опубликована: Май 3, 2023

Vibrio cholerae is a pathogen that causes disease in millions of people every year by colonizing the small intestine and then secreting potent cholera toxin. How overcomes colonization barrier created host’s natural microbiota is, however, still not well understood. In this context, type VI secretion system (T6SS) has gained considerable attention given its ability to mediate interbacterial killing. Interestingly, contrast non-pandemic or environmental V. isolates, strains are causing ongoing pandemic (7PET clade) considered T6SS-silent under laboratory conditions. Since idea was recently challenged, we performed comparative vitro study on T6SS activity using diverse regulatory mutants. We show modest detectable most tested competition The system’s also observed through immunodetection tube protein Hcp culture supernatants, phenotype can be masked strains’ haemagglutinin/protease. further investigated low within bacterial populations imaging 7PET at single-cell level. micrographs showed production machinery only fraction cells population. This sporadic higher 30 °C than 37 occurred independently known regulators TfoX TfoY but dependent VxrAB two-component system. Overall, our work provides new insight into heterogeneity possible explanation bulk measurements.

Язык: Английский

Процитировано

2
Interactions between pili affect the outcome of bacterial competition driven by the type VI secretion system DOI Creative Commons
Simon B. Otto, Richard Servajean, Alexandre Lemopoulos

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 26, 2023

Abstract The bacterial type VI secretion system (T6SS) is a widespread, kin-discriminatory weapon capable of shaping microbial communities. Due to the system’s dependency on contact, cellular interactions can lead either competition or kin protection. Cell-to-cell contact often accomplished via surface-exposed IV pili (T4P). In Vibrio cholerae, these T4P facilitate specific when bacteria colonize natural chitinous surfaces. However, it has remained unclear whether and, if so, how affect bacterium’s T6SS-mediated killing. this study, we demonstrate that pilus-mediated be harnessed reduce population V. cholerae under liquid growth conditions in T6SS-dependent manner. We also show naturally occurring diversity determines likelihood cell-to-cell consequently, extent competition. To determine factors enable hinder T6SS’s targeted reduction competitors carrying pili, developed physics-grounded computational model for autoaggregation. Collectively, our research demonstrates involved impose selective burden encounters non-kin cells possess an active T6SS. Additionally, study underscores significance protecting closely related individuals from T6SS attacks through autoaggregation and spatial segregation.

Язык: Английский

Процитировано

1