Relationship between the effects of heat pre-treatment on anaerobic performance of pig manure and the microbial variation within reactors

Chemical Engineering Journal, Год журнала: 2023, Номер 461, С. 141991 - 141991

Опубликована: Фев. 18, 2023

Язык: Английский

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Organic matter accumulation drives methylotrophic methanogenesis and microbial ecology in a hypersaline coastal lagoon

Limnology and Oceanography, Год журнала: 2024, Номер 69(9), С. 1970 - 1983

Опубликована: Июль 20, 2024

Abstract Hypersalinity is common in coastal wetlands throughout warm, tropical, and arid regions. Climate‐induced changes rainfall, sea level, anthropogenic modification to basins coastlines are likely further increase salinization these ecosystems. Yet, carbon cycling hypersaline not well understood, poorly constrained climate models. In the Coorong, a eutrophic, lagoon, recognized as internationally important under Ramsar convention, organic matter rapidly accumulates deeper areas of through settling fine detrital particles, phytoplankton suspended sediments. During initial surveys, elevated surface water methane (CH 4 ) concentrations were observed above depositional To identify drivers CH production, sediment characteristics assessed Genetic markers (i.e., 16rDNA mcrA functional gene) used characterize microbial communities. With multiple lines evidence, this study identifies matter, methanogen abundance, salinity which concentrated zones. Archaea also more abundant zones, including methylotrophic methanogens: Methanofastidiosales, Methanomasiliicoccales, Methermicoccaceae , Methanococcoides . These methanogens highly correlated porewater, suggesting an influence methanogenesis. investigate further, metabolic genes predicted from 16S rRNA with PICRUSt2. This represents first effort analyze dynamics underscoring need integrate unique ecosystems into global models enhance our understanding greenhouse gas emissions changing climate.

Язык: Английский

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Microbial carbon oxidation in seawater below the hypoxic threshold

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 22, 2025

Global oxygen minimum zones (OMZs) often reach hypoxia but seldom anoxia. Recently it was reported that Michaelis Menten constants (Km) of oxidative enzymes are orders magnitude higher than respiratory Km values, and in the Hypoxic Barrier Hypothesis proposed that, ecosystems experiencing falling oxygen, oxygenase enzyme activities become oxygen-limited long before respiration. We conducted a mesocosm experiment with phytoplankton bloom as an organic carbon source controlled dissolved (DO) concentrations dark to determine whether slows oxidation decline. Total utilization (TOU) hypoxic treatment (ca. 7.1 µM O2) 21.7% lower oxic 245.1 over first 43 days experiment. In addition, following restoration fully conditions treatment, TOU accelerated, demonstrating processes sensitive DO found large volumes ocean. Microbial amplicon-based community composition diverged between treatments, indicating specialized microbiome included Thioglobaceae (SUP05 Gammaproteobacteria), OM190 (Planctomycetota), ABY1 (Patescibacteria), SAR86 subclade D2472, thrived while genus Candidatus Actinomarina SAR11 alphaproteobacteria were sharply inhibited. Our findings support hypothesis kinetics might slow progression ocean deoxygenation oxygen-poor regions be factor evolution microbial taxa adapted environments.

Язык: Английский

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Mesophilic anaerobic digestion of mixed sludge in CSTR and AnMBR systems: A perspective on microplastics fate

Journal of Environmental Management, Год журнала: 2025, Номер 375, С. 124250 - 124250

Опубликована: Янв. 29, 2025

Язык: Английский

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Minisyncoccus archaeiphilus gen. nov., sp. nov., a mesophilic, obligate parasitic bacterium and proposal of Minisyncoccaceae fam. nov., Minisyncoccales ord. nov., Minisyncoccia class. nov. and Minisyncoccota phyl. nov. formerly referred to as Candidatus Patescibacteria or candidate phyla radiation

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Год журнала: 2025, Номер 75(2)

Опубликована: Фев. 10, 2025

In the domain Bacteria , one of largest, most diverse and environmentally ubiquitous phylogenetic groups, Candidatus Patescibacteria (also known as candidate phyla radiation/CPR), remains poorly characterized, leaving a major knowledge gap in microbial ecology. We recently discovered novel cross-domain symbiosis between Ca . Archaea highly purified enrichment cultures proposed taxa for characterized species, including Minisyncoccus archaeophilus corresponding family Minisyncoccaceae. this study, we report isolation bacterium, designated strain PMX.108 T two-strain co-culture with host archaeon, Methanospirillum hungatei DSM 864 (JF-1 ), hereby describe it first representative species Patescibacteria. Strain was isolated from mesophilic methanogenic sludge an anaerobic laboratory-scale bioreactor treating synthetic terephthalate- dimethyl terephthalate-manufacturing wastewater. The could not grow axenically is obligately parasitic, strictly depending on M. host. genome comparatively large (1.54 Mbp) compared to other members clade, lacked some genes involved biosynthesis pathway encoded type IV pili-related associated parasitic lifestyle ultrasmall microbes. G+C content genomic DNA 36.6 mol%. Here, phenotypic properties ; propose archaeiphilus gen. nov., sp. nov. accommodate strain. (=JCM 39522 ). also family, order, class phylum Minisyncoccaceae fam. Minisyncoccales Minisyncoccia class. Minisyncoccota phyl. within bacterial kingdom Bacillati

Язык: Английский

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Metagenomic Composition and Predicted Metabolic Pathway Analyses of the Endometrial and Rectal Microbiota in Dairy Cows Following the Introduction of a Complex Feed Additive

Frontiers in Bioscience-Elite, Год журнала: 2025, Номер 17(1)

Опубликована: Янв. 22, 2025

Background: The microbiome composition in dairy cows (Bos taurus) directly impacts on health and reproductive performance. This study aimed to determine the metagenomic predicted microbial community functions endometrium rectal chyme of fed a complex feed additive (CFA). latter included Bacillus mucilaginosus 159 strain, short-chain fatty acid, plus essential oils. Methods: Clinically healthy were divided into two groups (n = 15 each): (I) control group standard diet, (II) an experimental group. CFA was introduced diet Group II during entire transit period at dose 50 g per animal day; moreover, all animals received Pen-Strep 400 antibiotics prevent endometritis other pathologies. from biotopes assessed using targeted next-generation sequencing. Results: Significant changes observed metabolic pathways due administration, with endometrial microbiota being more responsive than intestinal microbiome. Remarkably, Actinobacteriota representatives disappeared compared controls, whose content ranged 0.34 3.3%. use also resulted less pronounced effect four for degradation catechol controls (p < 0.05). Conclusions: Our findings support concept relationship between gut system microflora cows, as we after orally administering CFA. emphasizes need integrated approach combining correction microecological disorders intestines simultaneously.

Язык: Английский

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Environmental instability reduces shock resistance by enriching specialist taxa with distinct two component regulatory systems

npj Biofilms and Microbiomes, Год журнала: 2025, Номер 11(1)

Опубликована: Март 31, 2025

Abstract Different microbial communities are impacted disproportionately by environmental disturbances. The degree to which a community can remain unchanged under disturbance is referred as resistance 1 . However, the contributing ecological factors, infer community’s unknown. In this study, impact of historical stability on phenomena and shocks was investigated. Three separate methanogenic bioreactor consortia, were subjected varying degrees stability, displayed different levels an organic loading rate (OLR) shock sampled. Their composition assessed using high throughput sequencing 16S rRNA genes assembly based metagenomics. effect instability such assembly, niche breadth rare biosphere in context each reactor’s demonstrated OLR shock. Additionally, metagenome assembled genomes analysed for functional effects prolonged stability/instability. system more experienced temporal variation beta diversity proliferation specialists, with abundant two component regulatory systems. This susceptible deterministic lower resistance, indicating that experiencing longer term (e.g. variations pH or temperature) less able resist large disturbance.

Язык: Английский

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Candidate Phyla Radiation (CPR) bacteria from hyperalkaline ecosystems provide novel insight into their symbiotic lifestyle and ecological implications

Microbiome, Год журнала: 2025, Номер 13(1)

Опубликована: Апрель 7, 2025

Abstract Background Candidate Phyla Radiation (CPR) represents a unique superphylum characterized by ultra-small cell size and symbiotic lifestyle. Although CPR bacteria have been identified in varied environments, their broader distribution, associations with hosts, ecological roles remain largely unexplored. To address these knowledge gaps, serpentinite-like environment was selected as simplified model system to investigate the communities hyperalkaline environments association hosts extreme conditions. Additionally, enzymatic activity, global evolution of CPR-derived genes encoding essential metabolites (e.g., folate or vitamin B 9 ) were analyzed assessed. Results In highly alkaline ecosystem (pH = 10.9–12.4), metagenomic analyses water sediment samples revealed that constituted 1.93–34.8% microbial communities. Metabolic reconstruction 12 high-quality metagenome-assembled genomes (MAGs) affiliated novel taxa from orders UBA6257, UBA9973, Paceibacterales suggests lack complete biosynthetic pathways for amino acids, lipids, nucleotides. Notably, commonly harbored associated cofactor biosynthesis metabolism, including dihydrofolate reductase ( folA ), serine hydroxymethyltransferase glyA methylenetetrahydrofolate folD ). two presumed auxotrophic incapable forming tetrahydrofolate (THF) due absence , potential some harboring genes. The functionality experimentally verified heterologous expression -deletion mutant Escherichia coli MG1655 Δ . Further assessment available n 4,581) proteins synthesis bioactive derivatives and/or genes) present 90.8% examined. It widespread metabolic complementarity between hosts. Conclusions This finding deepens our understanding mechanisms CPR-host symbiosis, providing insight into cofactor-dependent mutualistic interactions. Our observations suggest may contribute organisms indirectly influence biogeochemical processes.

Язык: Английский

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Anaerobic digestion at high-pH and alkalinity for biomethane production: Insights into methane yield, biomethane purity, and process performance

Bioresource Technology, Год журнала: 2025, Номер unknown, С. 132505 - 132505

Опубликована: Апрель 1, 2025

Язык: Английский

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Microbial diversity and capacity for arsenic biogeochemical cycling in aquifers associated with thermal mobilization

The Science of The Total Environment, Год журнала: 2025, Номер 977, С. 179357 - 179357

Опубликована: Апрель 15, 2025

Thermal recovery technologies for in-situ bitumen extraction can result in the heating of surrounding aquifers, potentially mobilizing arsenic naturally present sediments to groundwater. The relative toxicity dissolved is related its speciation, with As(V) being less toxic than As(III). Microorganisms have various mechanisms detoxification and metabolism, which include genes efflux, methylation, reduction/oxidation As(V)/As(III). We characterized microbial communities along two aquifer transects associated thermally mobilized near Northeastern Alberta oil sands deposits. 16S rRNA amplicons metagenomic sequencing data biomass from filtered groundwater indicated major changes dominant taxa between wells, especially those currently experiencing elevated concentrations. Annotation arsenic-related that efflux pumps (arsB, acr3), intracellular reduction (arsC) methylation (arsM) were widespread among community members but comparatively few organisms encoded respiratory reductases (arrA) oxidases (arxA, aioA). While this indicates microbes capacity exacerbate by increasing concentration As(III), some populations iron oxidizing sulfate reducing bacteria (including novel Gallionella Thermodesulfovibrionia populations) show potential indirect bioremediation through formation insoluble iron/sulfide minerals adsorb or coprecipitate arsenic. An unusually high proportional abundance a single Paceibacteria population lacked resistance was identified one high‑arsenic well, we discuss hypotheses ability persist. Overall, study describes how respond thermal plumes, predicts contributions biogeochemical cycling under disturbance.

Язык: Английский

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