Machine Learning Analysis of Magnetic Covalent Organic Framework Based Heterostructures Extracted Intracellular Metabolic Fingerprint for Direct Hypervirulent Klebsiella Pneumoniae Prediction DOI
Yongqi Wang,

Jiacheng Ye,

Rongrong Chen

и другие.

Talanta, Год журнала: 2025, Номер unknown, С. 127999 - 127999

Опубликована: Март 1, 2025

Язык: Английский

Global emergence of Carbapenem-resistant Hypervirulent Klebsiella pneumoniae driven by an IncFIIK34 KPC-2 plasmid DOI Creative Commons
Jianping Jiang, Leilei Wang, Yiyi Hu

и другие.

EBioMedicine, Год журнала: 2025, Номер 113, С. 105627 - 105627

Опубликована: Март 1, 2025

SummaryBackgroundCarbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) has been increasingly reported worldwide, posing a severe challenge to public health; however, the mechanisms driving its emergence and global dissemination remain unclear.MethodsWe analysed CR-hvKp strains derived from canonical hvKp backgrounds, acquired carbapenemase-encoding gene. These were identified 485 CRKp isolates in CRACKLE-2 China cohort, 259 multi-centre study, 67,631 K. genomes available GenBank. Clinical harbouring IncFIIK34 KPC-2 plasmid selected for genome sequencing, RNA-Seq, conjugation assays, vivo, ex vitro phenotypic characterisation.FindingsAnalysis of clinical 414 24 countries GenBank an as prevalent KPC (detected 25%, 45/178 KPC-producing CR-hvKp). Compared with epidemic IncFIIK2 plasmid, exhibited 100- 1000-fold increase frequency (10−4–10−5 vs. 10−7) growth advantage under meropenem challenge–likely due overexpression conjugation-related genes increased blaKPC copy number expression. transconjugants carrying this often reduced mucoviscosity, while retaining hypervirulence both murine models human neutrophil assays.InterpretationThe may be key factor CR-hvKp, underscoring urgent need enhanced molecular surveillance emerging pathogen.FundingNational Natural Science Foundation National Institutes Health.

Язык: Английский

Процитировано

3
Carbapenemase-Producing Enterobacterales from Patients Arriving from Ukraine in Poland, March 2022–February 2023 DOI Creative Commons
Marta Biedrzycka, Radosław Izdebski, Waleria Hryniewicz

и другие.

Infectious Diseases and Therapy, Год журнала: 2025, Номер unknown

Опубликована: Янв. 5, 2025

Despite a scarcity of data, before 2022 Ukraine was already considered high-prevalence country for carbapenemase-producing Enterobacterales (CPE), and the situation has dramatically worsened during full-scale war with Russia. The aim this study to analyse CPEs isolated in Poland from victims Ukraine. included 65 CPE isolates March till February 2023, recovered 36 Polish medical centres 57 patients arriving Ukraine, differing largely by age reason hospitalisation. All were sequenced MiSeq ten Klebsiella pneumoniae also MinION. Taxonomy, clonality resistomes analysed all CPEs, whereas phylogeny, serotypes, virulomes plasmids characterised K. pneumoniae, partially Escherichia coli ST131, using various bioinformatic tools. Multifactorial diversity reflected patients' clinical-epidemiological heterogeneity. represented six species. most prevalent 50 15 sequence types (STs), mainly ST395, ST307, ST11, ST147 ST23, producing NDM (-1/-5), OXA-48 (-48/-1242) or KPC (-2/-3)-like carbapenemases. Each STs produced groups loosely related isolates, clusters close relatives and/or unique correlating K serotypes Many these, especially NDM-1- OXA-48-producing ST395 Russian organisms. Others, example, NDM-1-producing clustered those Poland. Numerous had specific virulence genes, including aerobactin iuc, due spread pNDM-MAR plasmids, showing both resistance virulence. Two E. ST131 belonged clades B C1 KPC-3 NDM-1, respectively. Together similar studies Germany Netherlands, work documented broad dissemination driven number lineages circulating over large territory Eastern Europe.

Язык: Английский

Процитировано

2
Hypervirulent Klebsiella pneumoniae: Epidemiology outside Asian countries, antibiotic resistance association, methods of detection and clinical management DOI Creative Commons
Silvia García-Cobos, Jesús Calatayud, Marı́a Pérez-Vázquez

и другие.

Enfermedades Infecciosas y Microbiología Clínica, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Call for prudent use of the term hypervirulence in carbapenem-resistant Klebsiella pneumoniae DOI Creative Commons

Yongqiang Yang,

Alan McNally, Zhiyong Zong

и другие.

The Lancet Microbe, Год журнала: 2025, Номер unknown, С. 101090 - 101090

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1
Carbapenem-Resistant, Virulence Plasmid–Harboring Klebsiella pneumoniae, United States DOI Creative Commons
Jianping Jiang, Tengfei Long,

Adeline R. Porter

и другие.

Emerging infectious diseases, Год журнала: 2025, Номер 31(4)

Опубликована: Март 17, 2025

Carbapenem-resistant and virulence plasmid-harboring Klebsiella pneumoniae (pVir-CRKP) has emerged spread globally, yet clinical investigations from the United States remain limited. We conducted a genomic analysis of 884 unique carbapenem-resistant K. isolates multicenter US cohort identified 6 pVir-CRKP isolates, including 2 sequence type (ST) 23, ST893, ST11 isolates. Patients infected with experienced high Pitt bacteremia scores 33% 30-day mortality rate. The exhibited significant variation in genes plasmids, along differences mucoviscosity, capsule production, survival normal human serum, resistance to killing by polymorphonuclear neutrophils, vivo pathogenicity. Phylogenetic analyses showed that most were genetically similar strains reported other global regions. emergence higher potential carbapenem than predominant clone underscores need for active surveillance.

Язык: Английский

Процитировано

1
Genomic and phylogenetic analysis of hypervirulent Klebsiella pneumoniae ST23 in Ireland DOI Creative Commons
Maria Maguire,

Niall DeLappe,

Ciaran Clarke

и другие.

Microbial Genomics, Год журнала: 2025, Номер 11(3)

Опубликована: Март 19, 2025

Hypervirulent Klebsiella pneumoniae (hv Kp ) has emerged as a pathogen of global concern associated with invasive community-acquired infections. The combination hypervirulence and carbapenem resistance can result in severe difficult-to-treat This retrospective study aimed to investigate the spread hv sequence type 23 (ST23) Ireland convergence hypervirulent (hv) antimicrobial genotypes. Short-read sequences (PE300) for 90 K. ST23 isolates were generated by Galway Reference Laboratory Services (GRLS). Isolates from screening swabs ( n =59), infections =18), non-invasive sites =12) hospital environment =1). virulence content assessed genomically using Kleborate (v2.2.0), ABRicate (v1.0.1) Platon (v1.6). vivo was murine model. All genotypically 88/90 having maximal score 5 including carriage key genes. Eighty-two per cent (74/90) carried carbapenemase gene bla OXA-48 / OXA-181 NDM-1 ), 42% genes 3 or more classes. Core genomic delineation revealed be clonal similar profiles. Two distinct clusters Irish detected consisting 82/90 isolates. infection demonstrated virulence. An established clone is circulating within causing both colonization patients. lack reliable methods makes its detection control healthcare setting challenging.

Язык: Английский

Процитировано

1
Deciphering the relative importance of genetic elements in hypervirulent Klebsiella pneumoniae to guide countermeasure development DOI Creative Commons
Thomas A. Russo, Ulrike MacDonald,

Zachary J. Drayer

и другие.

EBioMedicine, Год журнала: 2024, Номер 107, С. 105302 - 105302

Опубликована: Авг. 23, 2024

Quantitating the contribution of phenotype-responsible elements in hypervirulent Klebsiella pneumoniae is needed.

Язык: Английский

Процитировано

8
Revisiting therapeutic options against Resistant Klebsiella Pneumoniae infection: phage therapy is key DOI
Jiabao Xing,

Rong-jia Han,

Jinxin Zhao

и другие.

Microbiological Research, Год журнала: 2025, Номер unknown, С. 128083 - 128083

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Successful Treatment of an Intra-Abdominal Abscess Caused by KPC-2-Producing Hypervirulent Klebsiella pneumoniae sequence type 11 with Imipenem/cilastatin/relebactam in a Japanese Patient DOI
Kazuhiro Ishikawa,

Koko Shibutani,

Yumiko Mikami

и другие.

Journal of Infection and Chemotherapy, Год журнала: 2025, Номер unknown, С. 102717 - 102717

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

1
Detection of cefiderocol and aztreonam/avibactam resistance in epidemic Escherichia coli ST-361 carrying bla NDM-5 and bla KPC-3 from foreign fighters evacuated from Ukraine DOI Creative Commons
Melissa J. Martin, Ting L. Luo,

Valentyn Kovalchuk

и другие.

Antimicrobial Agents and Chemotherapy, Год журнала: 2024, Номер unknown

Опубликована: Сен. 20, 2024

ABSTRACT Genomic surveillance detected clonal Escherichia coli sequence type-361 isolates carrying bla NDM-5 , KPC-3 CTX-M-15 and rmtB1 from a patient in Ukraine four wounded foreign soldiers evacuated to Germany. Isolates were non-susceptible carbapenems, aminoglycosides, cefiderocol aztreonam/avibactam due PBP3 YRIN insertion the CMY-145 AmpC β-lactamase. Coordinated efforts across civilian, military, veteran healthcare systems are essential prevent further spread as international volunteers return home after medical evacuation Ukraine.

Язык: Английский

Процитировано

5