Targeting Acinetobacter baumannii resistance-nodulation-division efflux pump transcriptional regulators to combat antimicrobial resistance
npj Antimicrobials and Resistance,
Год журнала:
2025,
Номер
3(1)
Опубликована: Янв. 25, 2025
Regulatory
elements
controlling
gene
expression
fine-tune
bacterial
responses
to
environmental
cues,
including
antimicrobials,
optimize
survival.
Acinetobacter
baumannii,
a
pathogen
notorious
for
antimicrobial
resistance,
relies
on
efficient
efflux
systems.
Though
the
role
of
systems
in
antibiotic
expulsion
are
well
recognized,
regulatory
mechanisms
their
remain
understudied.
This
review
explores
current
understanding
these
regulators,
aiming
inspire
strategies
combat
resistance
and
improve
therapeutic
outcomes.
Язык: Английский
Phenotypic and genetic heterogeneity of Acinetobacter baumannii in the course of an animal chronic infection
Microbial Genomics,
Год журнала:
2025,
Номер
11(2)
Опубликована: Фев. 19, 2025
Acinetobacter
baumannii
is
a
nosocomial
pathogen
associated
with
various
infections,
including
urinary
tract
infections
(UTIs).
In
the
course
of
an
infection,
A.
known
to
rapidly
become
resistant
antibiotic
therapy,
but
much
less
about
possible
adaptation
without
pressure.
Through
retrospective
study,
we
investigated
within-host
genetic
diversity
during
subclinical
5-year
UTI
in
animal-patient
after
withdrawal
colistin
treatment.
We
conducted
whole-genome
sequencing
and
phenotypic
assays
on
17
clonally
related
isolates
from
Sequence
Type
25
lineage.
Phylogenomic
analysis
revealed
their
proximity
animal
human
strains
same
country
suggesting
zoonotic
transmission
(France).
this
case
presented
variations
genome
sizes
nucleotide
sequences.
Over
underwent
reduction
through
insertion
sequence
(IS)
recombination,
phage
excision
or
plasmid
curing.
Alongside
global
reduction,
observed
expansion
IS17,
initially
located
endogenous
large
plasmid.
Genetic
were
mainly
biofilm
formation
metabolism
genes.
repeated
affecting
three
genes
two
adhesion
operons
weak
biofilm-forming
capacity.
Conversely,
only
metabolic
recurrently
affected,
indicated
rather
stable
profile
between
minor
adaptations
its
host.
Lastly,
overall
decreased
resistance
-
expected
absence
treatment
contrasted
conserved
due
pmrB
mutation
among
isolates.
Язык: Английский
Comprehensive molecular epidemiology of Acinetobacter baumannii from diverse sources in Nigeria
BMC Microbiology,
Год журнала:
2025,
Номер
25(1)
Опубликована: Март 31, 2025
Abstract
Background
Acinetobacter
baumannii
,
a
Gram-negative
bacterium,
is
public
health
threat
due
to
its
role
in
nosocomial
infections
and
increasing
antibiotic
resistance.
In
Nigeria,
data
on
the
molecular
epidemiology
of
A.
scarce.
This
study
investigates
genetic
diversity
presence
antimicrobial
resistance
determinants
virulence-related
genes
whole-genome
sequencing
189
Nigerian
isolates
deposited
repositories.
Genotypes
were
determined
in-silico
by
multilocus
sequence
typing
(MLST)
core
genome
MLST
(cgMLST).
Further,
(AMR)
analyzed.
Results
Most
(57.67%)
originated
from
South-west
Nigeria.
Isolates
human
origin
accounted
for
33.86%,
while
environmental
sources
comprised
6.87%,
59.27%
lacked
information
source
isolation.
The
cgMLST
analysis
revealed
multitude
genomic
lineages
circulating
Oxford
scheme
identified
44
types
(STs)
62.96%
strains,
with
ST1089
being
most
prevalent.
Pasteur
could
assign
95.77%
strains
49
STs,
ST2(IC2)
ST85(IC9)
dominant.
Antimicrobial
detected
168
encoding
12
classes,
cephalosporin,
carbapenem,
aminoglycoside
Notably,
bla
ADC−79
(23.81%),
OXA−23
(30.69%),
aph
(3″)-Ib
(30%)
frequent
variants
encountered.
Seventeen
multi-efflux
system
conferring
multiple
classes
identified.
Virulence
gene
137
six
mechanisms,
nutritional
factors,
effector
delivery
systems,
biofilm
production
Conclusion
highlights
AMR
virulence
emphasizing
need
ongoing
surveillance
inform
infection
control
develop
management
strategies.
Язык: Английский
Unveiling transposon-mediated multidrug resistance in OXA-23-producing Acinetobacter baumannii ST79/ST233 subclone KL9-OCL10 in Brazil
Gene,
Год журнала:
2025,
Номер
958, С. 149489 - 149489
Опубликована: Апрель 15, 2025
Язык: Английский
An Acinetobacter baumannii nasal carriage isolate recovered from an asymptomatic patient in Vietnam is extensively antibiotic resistant and produces a rare K71 type capsule
Microbiology Spectrum,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 22, 2024
is
a
leading
cause
of
multidrug-resistant
bacterial
infections
worldwide,
and
the
capsular
polysaccharide
(CPS)
major
virulence
determinant.
A
previous
study
Язык: Английский
Genomic analysis of early ST32 Acinetobacter baumannii strains recovered in US military treatment facilities reveals distinct lineages and links to the origins of the Tn6168 ampC transposon
Journal of Antimicrobial Chemotherapy,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 16, 2024
Abstract
Objectives
To
study
the
population
structure
and
genomic
characteristics,
including
antimicrobial
resistance
genes,
plasmid
types
surface
polysaccharide
type,
of
globally
distributed
Acinetobacter
baumannii
belonging
to
ST32
(Institut
Pasteur
scheme).
Methods
Antibiotic
phenotype
for
19
antibiotics
was
determined
using
Vitek
2.
Whole-genome
sequencing
performed
Illumina
MiSeq
platform.
Genomes
were
assembled
Newbler.
Phylogenetic
analysis
done
by
determining
core-genome
alignments
Panaroo
v1.3,
analysed
in
IQ-Tree2
v2.2.0.3
construct
Maximum
Likelihood
trees
RaxML
software.
Resistance
genes
IS
identified
Abricate
programme,
ISFinder
databases.
Results
One
hundred
thirty-three
(n
=
133)
A.
isolates
this
study.
These
genomes
originated
mainly
from
US
military
treatment
facilities
113),
but
also
included
additional
publicly
available
GenBank
20)
recovered
a
broad
geographic
distribution
extending
Asia
South
America.
all
133
revealed
at
least
four
clades,
with
over
80
forming
tightly
clustered
branch,
suggesting
they
are
likely
represent
outbreak
strains.
Analysis
ampC
region
showed
that
strains
played
significant
role
formation
widely
transposon,
Tn6168,
supplying
DNA
segments
containing
an
ISAba1-ampC
ST32s
via
homologous
recombination.
Conclusions
evolution
antibiotic
several
sequence
ST1
(global
clone
1)
ST3.
Язык: Английский