Soil Biology and Biochemistry,
Год журнала:
2019,
Номер
138, С. 107571 - 107571
Опубликована: Авг. 22, 2019
Global
fire
regimes
are
changing,
with
increases
in
wildfire
frequency
and
severity
expected
for
many
North
American
forests
over
the
next
100
years.
Fires
can
result
dramatic
changes
to
carbon
(C)
stocks
restructure
plant
microbial
communities,
long-lasting
effects
on
ecosystem
functions.
We
investigated
soil
communities
(bacteria
fungi)
an
extreme
season
northwestern
Canadian
boreal
forest,
using
field
surveys,
remote
sensing,
high-throughput
amplicon
sequencing
upland
wetland
sites.
hypothesized
that
vegetation
community
pH
would
be
most
important
determinants
of
composition,
while
effect
might
not
significant,
found
occurrence,
along
community,
moisture
regime,
pH,
total
carbon,
texture
all
significant
predictors
composition.
Burned
become
increasingly
dissimilar
unburned
severe
burns,
burn
index
(an
fractional
area
consumed
organic
soils
exposed
mineral
soils)
best
predicted
bacterial
whether
a
site
was
burned
or
predictor
fungi.
Globally
abundant
taxa
were
identified
as
positive
responders
this
system,
including
bacteria
Massilia
sp.
(64
×
more
fire)
Arthrobacter
(35
),
fungi
Penicillium
(22
)
Fusicladium
(12
).
Bacterial
fungal
co-occurrence
network
modules
characterized
by
responsiveness
well
regime.
Building
efforts
previous
studies,
our
results
consider
particularly
wide
range
soils,
vegetation,
severities,
we
identify
specific
fire-responsive
suggest
accounting
improves
understanding
response
fires.
The
accuracy
of
microbial
community
surveys
based
on
marker-gene
and
metagenomic
sequencing
(MGS)
suffers
from
the
presence
contaminants—DNA
sequences
not
truly
present
in
sample.
Contaminants
come
various
sources,
including
reagents.
Appropriate
laboratory
practices
can
reduce
contamination,
but
do
eliminate
it.
Here
we
introduce
decontam
(
https://github.com/benjjneb/decontam
),
an
open-source
R
package
that
implements
a
statistical
classification
procedure
identifies
contaminants
MGS
data
two
widely
reproduced
patterns:
appear
at
higher
frequencies
low-concentration
samples
are
often
found
negative
controls.
Decontam
classified
amplicon
sequence
variants
(ASVs)
human
oral
dataset
consistently
with
prior
microscopic
observations
taxa
inhabiting
environment
previous
reports
contaminant
taxa.
In
metagenomics
measurements
dilution
series,
substantially
reduced
technical
variation
arising
different
protocols.
application
to
recently
published
datasets
corroborated
extended
their
conclusions
little
evidence
existed
for
indigenous
placenta
microbiome
some
low-frequency
seemingly
associated
preterm
birth
were
contaminants.
improves
quality
by
identifying
removing
DNA
sequences.
integrates
easily
existing
workflows
allows
researchers
generate
more
accurate
profiles
communities
no
additional
cost.
Nature,
Год журнала:
2017,
Номер
551(7681), С. 457 - 463
Опубликована: Окт. 31, 2017
Abstract
Our
growing
awareness
of
the
microbial
world’s
importance
and
diversity
contrasts
starkly
with
our
limited
understanding
its
fundamental
structure.
Despite
recent
advances
in
DNA
sequencing,
a
lack
standardized
protocols
common
analytical
frameworks
impedes
comparisons
among
studies,
hindering
development
global
inferences
about
life
on
Earth.
Here
we
present
meta-analysis
community
samples
collected
by
hundreds
researchers
for
Earth
Microbiome
Project.
Coordinated
new
methods,
particularly
use
exact
sequences
instead
clustered
operational
taxonomic
units,
enable
bacterial
archaeal
ribosomal
RNA
gene
to
be
followed
across
multiple
studies
allow
us
explore
patterns
at
an
unprecedented
scale.
The
result
is
both
reference
database
giving
context
sequence
data
framework
incorporating
from
future
fostering
increasingly
complete
characterization
Earth’s
diversity.
Nature Communications,
Год журнала:
2020,
Номер
11(1)
Опубликована: Май 12, 2020
Abstract
Microplastics
are
ubiquitous
in
estuarine,
coastal,
and
deep
sea
sediments.
The
impacts
of
microplastics
on
sedimentary
microbial
ecosystems
biogeochemical
carbon
nitrogen
cycles,
however,
have
not
been
well
reported.
To
evaluate
if
influence
the
composition
function
communities,
we
conducted
a
microcosm
experiment
using
salt
marsh
sediment
amended
with
polyethylene
(PE),
polyvinyl
chloride
(PVC),
polyurethane
foam
(PUF)
or
polylactic
acid
(PLA)
microplastics.
We
report
that
presence
alters
community
cycling
processes.
Compared
to
control
sediments
without
microplastic,
PUF-
PLA-amended
promote
nitrification
denitrification,
while
PVC
amendment
inhibits
both
These
results
indicate
processes
can
be
significantly
affected
by
different
microplastics,
which
may
serve
as
organic
substrates
for
communities.
Considering
this
evidence
increasing
microplastic
pollution,
impact
plastics
global
merits
critical
investigation.
PLoS Computational Biology,
Год журнала:
2017,
Номер
13(2), С. e1005404 - e1005404
Опубликована: Фев. 21, 2017
Community-level
data,
the
type
generated
by
an
increasing
number
of
metabarcoding
studies,
is
often
graphed
as
stacked
bar
charts
or
pie
graphs
that
use
color
to
represent
taxa.
These
graph
types
do
not
convey
hierarchical
structure
taxonomic
classifications
and
are
limited
for
categories.
As
alternative,
we
developed
metacoder,
R
package
easily
parsing,
manipulating,
graphing
publication-ready
plots
data.
Metacoder
includes
a
dynamic
flexible
function
can
parse
most
text-based
formats
contain
classifications,
taxon
names,
identifiers,
sequence
identifiers.
then
subset,
sample,
order
this
parsed
data
using
set
intuitive
functions
take
into
account
nature
Finally,
extremely
plotting
enables
quantitative
representation
up
4
arbitrary
statistics
simultaneously
in
tree
format
mapping
size
nodes
edges.
also
allows
exploration
barcode
primer
bias
integrating
run
digital
PCR.
Although
it
has
been
designed
from
research,
metacoder
be
applied
any
component
such
gene
ontology
geographic
location
Our
complements
currently
available
tools
community
analysis
provided
open
source
with
extensive
online
user
manual.
Sequence-based
approaches
to
study
microbiomes,
such
as
16S
rRNA
gene
sequencing
and
metagenomics,
are
uncovering
associations
between
microbial
taxa
a
myriad
of
factors.
A
drawback
these
is
that
the
necessary
library
preparation
bioinformatic
analyses
complicated
continuously
changing,
which
can
be
barrier
for
researchers
new
field.
We
present
three
essential
components
conducting
microbiome
experiment
from
start
finish:
first,
simplified
step-by-step
custom
protocol
requires
limited
lab
equipment,
cost-effective,
has
been
thoroughly
tested
utilized
on
various
sample
types;
second,
series
scripts
integrate
commonly
used
tools
available
standalone
installation
or
single
downloadable
virtual
image;
third,
set
workflows
tutorials
provide
guidance
education
those
This
resource
will
foundations
newly
entering
field
much-needed
best
practices
ensure
quality
research
undertaken.
All
protocols,
scripts,
workflows,
tutorials,
images
freely
through
Microbiome
Helper
website
(https://github.com/mlangill/microbiome_helper/wiki).
IMPORTANCE
As
continues
grow,
multitude
learning
how
conduct
proper
experiments.
outline
here
streamlined
approach
processing
samples
detailed
construction
standard
operating
procedures.
allows
rapid
reliable
analysis,
allowing
focus
more
their
design
results.
Our
bundled
software
Helper.
evolve,
updated
with
training
materials.
Cell,
Год журнала:
2021,
Номер
184(15), С. 3884 - 3898.e11
Опубликована: Июнь 17, 2021
Immune-microbe
interactions
early
in
life
influence
the
risk
of
allergies,
asthma,
and
other
inflammatory
diseases.
Breastfeeding
guides
healthier
immune-microbe
relationships
by
providing
nutrients
to
specialized
microbes
that
turn
benefit
host's
immune
system.
Such
bacteria
have
co-evolved
with
humans
but
are
now
increasingly
rare
modern
societies.
Here
we
show
a
lack
bifidobacteria,
particular
depletion
genes
required
for
human
milk
oligosaccharide
(HMO)
utilization
from
metagenome,
is
associated
systemic
inflammation
dysregulation
life.
In
breastfed
infants
given
Bifidobacterium
infantis
EVC001,
which
expresses
all
HMO-utilization
genes,
intestinal
T
helper
2
(Th2)
Th17
cytokines
were
silenced
interferon
β
(IFNβ)
was
induced.
Fecal
water
EVC001-supplemented
contains
abundant
indolelactate
B.
infantis-derived
indole-3-lactic
acid
(ILA)
upregulated
immunoregulatory
galectin-1
Th2
cells
during
polarization,
functional
link
between
beneficial
immunoregulation
first
months