Dietary cholesterol drives fatty liver-associated liver cancer by modulating gut microbiota and metabolites

Gut, Год журнала: 2020, Номер 70(4), С. 761 - 774

Опубликована: Июль 21, 2020

Objective Non-alcoholic fatty liver disease (NAFLD)-associated hepatocellular carcinoma (HCC) is an increasing healthcare burden worldwide. We examined the role of dietary cholesterol in driving NAFLD–HCC through modulating gut microbiota and its metabolites. Design High-fat/high-cholesterol (HFHC), high-fat/low-cholesterol or normal chow diet was fed to C57BL/6 male littermates for 14 months. Cholesterol-lowering drug atorvastatin administered HFHC-fed mice. Germ-free mice were transplanted with stools from different diets determine direct modulated-microbiota NAFLD–HCC. Gut analysed by 16S rRNA sequencing serum metabolites liquid chromatography–mass spectrometry (LC–MS) metabolomic analysis. Faecal microbial compositions 59 hypercholesterolemia patients 39 healthy controls. Results High led sequential progression steatosis, steatohepatitis, fibrosis eventually HCC mice, concomitant insulin resistance. Cholesterol-induced formation associated dysbiosis. The composition clustered distinctly along stages steatohepatitis HCC. Mucispirillum, Desulfovibrio, Anaerotruncus Desulfovibrionaceae increased sequentially; while Bifidobacterium Bacteroides depleted which corroborated human hypercholesteremia patients. Dietary induced bacterial alteration including taurocholic acid decreased 3-indolepropionic acid. gavaged HFHC manifested hepatic lipid accumulation, inflammation cell proliferation. Moreover, restored cholesterol-induced dysbiosis completely prevented development. Conclusions drives inducing Cholesterol inhibitory therapy manipulation may be effective strategies prevention.

Язык: Английский

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Host–microbiota interactions in inflammatory bowel disease

Nature reviews. Immunology, Год журнала: 2020, Номер 20(7), С. 411 - 426

Опубликована: Янв. 31, 2020

Язык: Английский

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A Proinflammatory Gut Microbiota Increases Systemic Inflammation and Accelerates Atherosclerosis

Circulation Research, Год журнала: 2019, Номер 124(1), С. 94 - 100

Опубликована: Янв. 3, 2019

Rationale: Several studies have suggested a role for the gut microbiota in inflammation and atherogenesis. A causal relation relationship between microbiota, inflammation, atherosclerosis has not been explored previously. Objective: Here, we investigated whether proinflammatory from Caspase1 −/− ( Casp1 ) mice accelerates atherogenesis Ldlr mice. Method Results: We treated female with antibiotics subsequently transplanted them fecal based on cohousing approach. Autologous transplantation of served as control. Mice were cohoused 8 or 13 weeks fed chow high-fat cholesterol–rich diet. Fecal samples collected, factors related to metabolism, intestinal health, atherosclerotic phenotypes measured. Unweighted Unifrac distances 16S rDNA (ribosomal DNA) sequences confirmed introduction into (referred mice). Analysis lesion size aortic root demonstrated significant 29% increase plaque 13-week cholesterol–fed compared found increased numbers circulating monocytes neutrophils elevated cytokine levels plasma Neutrophil accumulation was enhanced 16S-rDNA-encoding sequence analysis feces identified reduction short-chain fatty acid–producing taxonomies Akkermansia , Christensenellaceae Clostridium Odoribacter Consistent these findings, cumulative concentrations anti-inflammatory acids propionate, acetate butyrate cecum significantly reduced Conclusions: Introduction enhances systemic

Язык: Английский

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Mucispirillum schaedleri Antagonizes Salmonella Virulence to Protect Mice against Colitis

Cell Host & Microbe, Год журнала: 2019, Номер 25(5), С. 681 - 694.e8

Опубликована: Апрель 18, 2019

Язык: Английский

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A High-Fat Diet Increases Gut Microbiota Biodiversity and Energy Expenditure Due to Nutrient Difference

Nutrients, Год журнала: 2020, Номер 12(10), С. 3197 - 3197

Опубликована: Окт. 20, 2020

A high-fat diet (HFD) can easily induce obesity and change the gut microbiota its metabolites. However, studies on effects of diets host have drawn inconsistent results. In this study, unexpected results showed that refined HFD increased diversity short-chain fatty acids (SCFAs), causing an increase in energy metabolism. Further analysis revealed these changes were caused by different fiber content two diets. Male C57BL/6J mice (4–5 weeks old) fed either or low-fat (LFD) for 14 weeks. The metabolic rates, thermogenesis, microbiome, intestinal SCFAs tested. triggered disturbed glucose homeostasis. Mice ingested more than LFD (p < 0.0001), higher SCFA concentrations related to abundances specific bacteria group. Also, heat up-regulated thermogenesis genes uncoupling protein 1(Ucp-1), peroxisome proliferator-activated receptor-γ coactivator-1α (Pgc-1α) expression brown adipose tissue (BAT). It was 16S rRNA gene sequencing microbial diversity, which enriched Desulfovibrionaceae, Rikenellaceae RC9 group, Mucispirillum, meanwhile, reduced abundance Lactobacillus, Bifidobacterium, Akkermansia, Faecalibaculum, Blautia. predicted pathways indicated non-absorbed carbohydrate metabolism pathways, as well risks colonization pathogens inflammation. conclusion, obesogenic male mice, intake from drove SCFAs, expenditure. Meanwhile, differences nutrient dissociate broad expenditure, microbiota, metabolites obesity, raising doubts previous studies. Therefore, it is necessary consider whether will interfere with experiments.

Язык: Английский

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The gut microbiota is largely independent of host genetics in regulating fat deposition in chickens

The ISME Journal, Год журнала: 2019, Номер 13(6), С. 1422 - 1436

Опубликована: Фев. 6, 2019

Язык: Английский

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The human symbiont Mucispirillum schaedleri: causality in health and disease

Medical Microbiology and Immunology, Год журнала: 2021, Номер 210(4), С. 173 - 179

Опубликована: Май 22, 2021

Язык: Английский

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Dietary betaine prevents obesity through gut microbiota-drived microRNA-378a family

Gut Microbes, Год журнала: 2021, Номер 13(1)

Опубликована: Янв. 1, 2021

Betaine is a natural compound present in commonly consumed foods and may have potential role the regulation of glucose lipids metabolism. However, underlying molecular mechanism its action remains largely unknown. Here, we show that supplementation with betaine contributes to improved high-fat diet (HFD)-induced gut microbiota dysbiosis increases anti-obesity strains such as

Язык: Английский

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Antioxidant and anti-inflammatory roles of tea polyphenols in inflammatory bowel diseases

Food Science and Human Wellness, Год журнала: 2022, Номер 11(3), С. 502 - 511

Опубликована: Фев. 4, 2022

Polyphenols, including phenolic acids, flavonoids, and procyanidins, are abundant in food beverage derived from plants. Tea (Camellia sinensis) is particularly rich polyphenols (e.g., catechins, theaflavins, thearubigins, gallic acid, flavonols), which thought to contribute the health benefits of tea. High intake tea has been described prevent and/or attenuate a variety chronic pathological conditions like cardiovascular diseases, neurodegenerative diabetes, cancer. This review focuses on established antioxidant anti-inflammatory properties underlying mechanisms their involvement inflammatory bowel diseases (IBD). act as efficient antioxidants by inducing an endogenous defense system maintaining intracellular redox homeostasis. also regulate signaling pathways such nuclear factor-κB, activator protein 1, signal transducer transcriptions, factor E2-related 2, associated with IBD development. Accumulating pieces evidence have indicated that enhance epithelial barrier function improve gut microbial dysbiosis, contributing management colitis. Therefore, this study suggests supplementation could outcome patients IBD.

Язык: Английский

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Akkermansia muciniphila exacerbates food allergy in fibre-deprived mice

Nature Microbiology, Год журнала: 2023, Номер 8(10), С. 1863 - 1879

Опубликована: Сен. 11, 2023

Abstract Alterations in the gut microbiome, including diet-driven changes, are linked to rising prevalence of food allergy. However, little is known about how specific bacteria trigger breakdown oral tolerance. Here we show that depriving specific-pathogen-free mice dietary fibre leads a microbiota signature with increases mucin-degrading bacterium Akkermansia muciniphila . This associated intestinal barrier dysfunction, increased expression type 1 and 2 cytokines IgE-coated commensals colon, which result an exacerbated allergic reaction allergens, ovalbumin peanut. To demonstrate causal role A. , employed tractable synthetic human gnotobiotic mice. The presence within microbiota, combined deprivation, resulted stronger anti-commensal IgE coating innate type-2 immune responses, worsened symptoms Our study provides important insights into microbes can regulate pathways allergy diet-dependent manner.

Язык: Английский

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