Biodegradation Potential of Microorganisms in Hydrocarbon-Polluted Baltic Sea Sediments
Опубликована: Янв. 1, 2025
The
spill
of
crude
oil
and
petroleum
derivatives
comprises
the
majority
oceans
pollution
leads
to
acute
chronic
damages
exposed
organisms
coastal
communities.
Some
bacteria
fungi
are
able
biodegrade
hydrocarbons
both
in
terrestrial
aquatic
environments.
Sediments
taken
from
Baltic
Sea
vicinity
one
terminal,
Curonian
Lagoon
strait
with
high
anthropogenic
shipping
pressure
a
source
microorganisms
having
potential
degrade
products.
Next
generation
sequencing
(NGS)
identified
taxa
(e.g.
Thalassolituus,
Exiguobacterium)
were
indicative
consistent
presence
sediments.
Through
enrichment
sediments
samples
oil,
marine
diesel
lubricating
line
NGS
study
results,
we
isolate
several
strains
oil-degrading
belonging
genera
Pseudomonas
Priestia,
members
family
Lysobacteriaceae;
as
well
Dipodoscaceae,
Dipodoscales
incertae
sedis,
Yarrowia,
Cyberlindnera,
Geotrichum,
Pichia,
Candida
Fusarium.
results
suggest
that
bacterial
fungal
communities
present
demonstrate
significant
for
bioremediation
other
pollutants.
Язык: Английский
Broad-spectrum hydrocarbon-degrading microbes in the global ocean metagenomes
The Science of The Total Environment,
Год журнала:
2024,
Номер
926, С. 171746 - 171746
Опубликована: Март 21, 2024
Язык: Английский
Characterization and identification of long-chain hydrocarbon-degrading bacterial communities in long-term chronically polluted soil in Ogoniland: an integrated approach using culture-dependent and independent methods
Environmental Science and Pollution Research,
Год журнала:
2024,
Номер
31(21), С. 30867 - 30885
Опубликована: Апрель 15, 2024
Escalating
oil
consumption
has
resulted
in
an
increase
accidental
spills
of
petroleum
hydrocarbons,
causing
severe
environmental
degradation,
notably
vulnerable
regions
like
the
Niger
Delta.
Complex
mixture
these
hydrocarbons
particularly
long-chain
alkanes
presents
unique
challenges
restoration
polluted
environment
due
to
their
chemical
properties.
This
study
aimed
investigate
hydrocarbon-degrading
bacterial
communities
within
long-term
chronically
soil
Ogoniland,
by
utilizing
both
traditional
cultivation
methods
and
modern
culture-independent
techniques.
Results
revealed
that
surface-polluted
(SPS)
subsurface
(SPSS)
exhibit
significantly
higher
total
organic
carbon
(TOC)
ranging
from
5.64
5.06%
(TPH)
levels
36,775
ppm
14,087
ppm,
compared
unpolluted
(UPS)
with
1.97%
TOC
479
TPH,
respectively.
Analysis
chain
lengths
reveals
prevalence
longer-chain
(C20-28)
surface
soil.
Culture-dependent
methods,
crude
enrichment
(COE)
paraffin
wax
(PWE),
yield
47
isolates
subjected
a
alkane
degradation
assay.
Twelve
strains
demonstrate
significant
abilities
across
all
enriched
media.
Three
members,
namely
Pseudomonas
sp.
(almA),
Marinomonas
Alteromonas
(ladA),
genes
responsible
for
demonstrating
efficiency
between
50
80%.
Culture-independent
analysis
SPS
samples
greater
species
richness
diversity
SPSS
samples.
Proteobacteria
dominates
as
phylum
sample
types,
22.23
82.61%,
Firmicutes
(0.2-2.22%),
Actinobacteria
(0.4-3.02%),
Acidobacteria
(0.1-3.53%)
also
prevalent.
Bacterial
profiles
at
genus
level
distinct
variations
among
populations
comprising
number
hydrocarbon
degraders
functional
predictions
highlight
presence
potential
catabolic
(nahAa,
adh2,
cpnA)
However,
culture-dependent
only
captured
few
dominant
members
found
analysis,
implying
more
specialized
media
or
environments
are
needed
isolate
members.
The
findings
this
contribute
valuable
information
ecological
biotechnological
aspects,
aiding
development
effective
bioremediation
applications
restoring
oil-contaminated
environments.
Язык: Английский
Toxicity of Soot Against Microorganisms Isolated from Artisanal Crude Oil Refining Sites in the Niger Delta
D. B. Onwuna,
H. O. Stanley,
Gideon O. Abu
и другие.
Deleted Journal,
Год журнала:
2024,
Номер
2(2), С. 166 - 173
Опубликована: Март 25, 2024
Background
and
Objective:
Soot
is
a
mass
of
impure
carbon,
considered
to
be
an
airborne
contaminant
in
areas
where
artisanal
crude
oil
refining
takes
place.This
study
aimed
isolate
microorganisms
from
soot-contaminated
plants,
water
soil
samples
within
the
vicinity
operations
Tombia
Kingdom,
Rivers
State,
Nigeria.Materials
Methods:
Isolates
were
identified
using
molecular
technique,
by
comparing
their
16S
rRNA
ITS
genes
with
previously
NCBI
data.A
biotoxicity
assay
was
conducted
determine
effect
different
concentrations
(1,10,100,
1000
mg/L)
soot
against
selected
isolates.Microbial
growth
monitored
for
24,
48,
72
96
hrs.Results:
Mean
heavy
metals
ranged
1.29±1.80-25.09±32.51mg/kg
during
wet
season
8.52±0.20-80.51±0.61mg/kg
dry
season.The
isolates
used
closely
matched
six
bacteria,
Enterobacter
asburiae
(61.6%),Pantoea
dispersa
(100%),
Kocuria
rhizophila
(98%),
Bacillus
cereus
(98.8%),Bacillus
subtilis
bugandensis
(100%)
one
fungus
Exophiala
dermatitidis
(100%).All
able
grow
presence
high
soot,
hrs.Conclusion:
Functional
hydrocarbon
degradation
(alkB
PAH)
detected
some
isolates.The
showed
tolerance
could
useful
bioremediation
polluted
soil.
Язык: Английский
Beyond methane, new frontiers in anaerobic microbial hydrocarbon utilizing pathways
Microbial Biotechnology,
Год журнала:
2024,
Номер
17(6)
Опубликована: Июнь 1, 2024
Alkanes,
single
carbon
methane
to
long-chain
hydrocarbons
(e.g.
hexadecane
and
tetradecane),
are
important
sources
anaerobic
microbial
communities.
In
anoxic
environments,
archaea
known
utilize
produce
via
the
methyl-coenzyme
M
reductase
enzyme
(MCR).
Recent
explorations
of
new
like
deep
sea
sediments,
that
have
coupled
metagenomics
cultivation
experiments
revealed
divergent
MCRs,
also
referred
as
alkyl-coenzyme
reductases
(ACRs)
in
archaea,
with
similar
mechanisms
C
Язык: Английский
Methane production from hydrocarbons by consortia dominated by ANME archaea
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 30, 2024
Understanding
microbial
production
and
consumption
of
methane,
a
potent
greenhouse
gas
in
the
atmosphere,
is
critical
for
bridging
knowledge
gaps
global
carbon
cycling.
In
anoxic
environments,
methane
known
to
be
produced
through
hydrogenotrophic,
acetoclastic
or
methylotrophic
mechanisms.
Methane
from
hydrocarbons
may
also
important,
especially
hydrocarbon-rich
like
Gulf
California,
but
mechanism
this
hydrocarbonoclastic
methanogenesis
remains
unclear.
The
activity
consortia
anaerobic
oxidizing
(ANME)
archaea
bacteria
limits
release
atmosphere
by
consuming
environments
globally.
Here
we
used
isotopic-labeling
track
conversion
(hexadecane
naphthalene)
enrichments
hydrothermally
impacted,
sediments
California.
was
directly
hexadecane
naphthalene,
both
presence
absence
sulfate.
We
reconstructed
metagenomic
assembled-genomes
(MAGs)
these
experiments
which
revealed
mixture
dominated
Desulfobacteriota
Bacteroidota,
Aeinigmarchaeota,
Thermoplasmatota,
ANME
group
2c.
ANME-2c
were
only
MAGs
that
encoded
methyl
coenzyme
M
reductases
(McrA)
complete
Wood-Ljungdahl
pathways
(WLP).
This
suggests
involved
along
seafloor,
our
understanding
roles
globally
important
microbes
not
yet
fully
appreciated.
Язык: Английский