Composition, Seasonal Dynamics and Metabolic Potential of the Rhizosphere Microbiome Associated with Wild White Poplar DOI Creative Commons

Mikhail I. Popchenko,

Dmitry S. Karpov, Natalya S. Gladysh

и другие.

BioTech, Год журнала: 2024, Номер 13(4), С. 52 - 52

Опубликована: Дек. 1, 2024

The white poplar (Populus alba) is a dioecious woody plant with significant potential for the phytoremediation of soils. To realize this potential, it necessary to utilize growth-promoting microorganisms. One source such beneficial microorganisms rhizosphere community wild-growing trees. However, structure, dynamics, and metabolism remain poorly understood. ascertain seasonal species diversity, metabolic we sequenced 16S rRNA genes in metagenomes derived from 165 soil samples collected spring autumn root surfaces 102 trees situated disparate geographical locations. three most prevalent phyla across all are Proteobacteria, Actinobacteriota, Acidobacteriota. At order level, orders Sphingomonadales Rhizobiales. Accordingly, families Sphingomonadaceae Rhizobiaceae were identified as dominant. rhizospheric microbiome exhibited substantial inter-seasonal variation. Six families, including Caulobacteraceae, Xanthomonadaceae, Chitinophagaceae, Chthoniobacteraceae, Sphingomonadaceae, Rhizobiaceae, alterations (spring-to-autumn) locations under study. Members family, which includes nitrogen-fixing bacteria, can provide plant-available forms nitrogen nitrate ammonium. may facilitate conversion inorganic sulfur into sulfur-containing amino acids, cysteine methionine, that bioavailable plants. Furthermore, capable synthesizing organic acids (including Krebs cycle acids), some lipids sugars. Consequently, stimulate growth by providing readily available sulfur, well building blocks synthesis proteins, nucleic other macromolecules. Many these pathways, fixation, subjected changes.

Язык: Английский

Resolving ecological drivers of temporal variations of β-diversity across intertidal microbiomes DOI Creative Commons
Xia Liu, Xiaofan Gong, Kai Ma

и другие.

ISME Communications, Год журнала: 2025, Номер unknown

Опубликована: Фев. 17, 2025

Resolving the ecological drivers mediating diversity patterns of microbial communities across space and through time is a central issue in ecology. Both regional species pools local community assembly contribute to spatial turnover biodiversity. In this study, we extended concept pool temporal, investigated seasonal dynamics intertidal microbiomes four domains/kingdoms (bacteria, archaea, fungi, protists). The results showed that variations β-diversity were primarily governed by processes rather than temporal pools. Different structured different processes, with homogeneous selection as major process for all them. Additionally, bacteria fungi critically shaped drift, protists drift dispersal. Among various factors, temperature was important shaping β-diversity. fluctuation strongly associated protists, resulting high composition. This study demonstrated dynamic β-variations microbiomes, expanding our understanding from

Язык: Английский

Процитировано

0

Seasonal Change in Microbial Diversity: Bile Microbiota and Antibiotics Resistance in Patients with Bilio-Pancreatic Tumors: A Retrospective Monocentric Study (2010–2020) DOI Creative Commons
Paola Di Carlo, Nicola Serra, Consolato Sergi

и другие.

Antibiotics, Год журнала: 2025, Номер 14(3), С. 283 - 283

Опубликована: Март 9, 2025

Background: Bilio-pancreatic tumors are a severe form of cancer with high rate associated mortality. These patients showed the presence bacteria such as Escherichia coli and Pseudomonas spp. in bile-pancreatic tract. Therefore, efficient antibiotic therapy is essential to reduce bacterial resistance adverse events patients. Recent studies on seasonality infectious diseases may aid developing effective preventive measures. This study examines seasonal impact bile microbiota composition its microorganisms hepato-pancreatic-biliary cancer. Methods: We retrospectively evaluated effect seasonally from 149 strains isolated by 90 Italian positive culture samples collected through endoscopic retrograde cholangiopancreatography between 2010 2020. Results: Across all seasons, most frequently found were E. coli, spp., Enterococcus Regarding resistance, highest 3GC, fluoroquinolones, aminoglycosides, fosfomycin, piperacillin-tazobactam summer lowest spring, except for carbapenems colistin. Conclusions: Antibiotic has negative effects who rely antibiotics prevent treat infections. Knowing whether fungal changes seasons key information define adequate more therapy.

Язык: Английский

Процитировано

0

The hidden life ofXylella: Mining the NCBI Sequence Read Archive reveals potential new species, host plants and infected areas for this elusive bacterial plant pathogen DOI Creative Commons
Martial Briand,

Marie Agnès Jacques,

Jessica Dittmer

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 20, 2025

ABSTRACT New crop disease outbreaks can occur when phytopathogenic bacterial pathogens acquire new traits, switch to a host plant or are introduced into geographic areas. Therefore, the rapid detection of pathogen in environment and/or is essential mitigate outbreaks. However, bacteria with wide range, many asymptomatic hosts and slow symptom development sometimes remain unnoticed for years. This case vector-borne xylem-inhabiting Xylella fastidiosa known infect hundreds species worldwide its sister X. taiwanensis , currently thought be restricted Taiwan. To investigate whether two already present other parts world, potentially unrecognised species, we performed an in-depth data mining raw sequence available NCBI Sequence Read Archive. led identification 62 datasets from diverse insect samples around world. Furthermore, nine draft one circular genome could assembled these datasets. Our results reveal several potential plants previously unrecognized infected areas Americas, Africa southeast Asia. Moreover, newly-assembled genomes represent strains both as well additional infecting wild rice. Taken together, our work extends knowledge on genetic diversity, range global distribution genus orient surveillance programs towards regions plants.

Язык: Английский

Процитировано

0

Dual-mycorrhizal colonization is determined by plant age and host identity in two species of Populus DOI
Jake Nash, Brian P. Looney, Melissa A. Cregger

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 29, 2025

Abstract Plants have evolved symbioses with mycorrhizal and endophytic fungi that are essential for their growth survival. While most plants associate a single guild of fungi, select group termed “dual-mycorrhizal plants” both arbuscular ectomycorrhizal fungi. Although shift from to colonization plant development has been demonstrated on other dual-mycorrhizal hosts, it is not known how shifts age in Populus species. We performed an in planta mycorrhization experiment test the occurrence switching response host-specific patterns fungal two species (P. tremuloides P. trichocarpa). found only trichocarpa displayed colonization, while associated but On trichocarpa, guilds increased abundance age, root decreased. Many early-colonizing we documented strong saprotrophic capabilities, which may be important trait fast colonization. Dark septate endophytes were more abundant than either likely functionally members community. Our findings represent novel pattern illustrate vary association results also stress importance “third guild” symbionts – dark plants.

Язык: Английский

Процитировано

0

Pseudomonas fluorescens Inoculation Enhances Salix matsudana Growth by Modifying Phyllosphere Microbiomes, Surpassing Nitrogen Fertilization DOI
Qing Li, Yue He, Jian Feng

и другие.

Plant Cell & Environment, Год журнала: 2024, Номер unknown

Опубликована: Сен. 19, 2024

ABSTRACT The enhancement of plant growth by soil fertilization and microbial inoculation involves different mechanisms, particularly altering the phyllosphere microbiome. This study investigated how nitrogen (N) fertilization, Pseudomonas fluorescens strain R124 their combined effects influence different‐aged Salix matsudana cuttings modulating N dynamics within Results showed that P. was significantly more effective than alone, enhancing biomass, nutrient uptake, content root development 90.51%, 18.18%, 72.74% 126.20%, respectively. Crucially, notably shifted beta‐diversity community, with K‐strategy fungi fixation subsequent growth. Cuttings from middle‐aged forests displayed robust those young‐aged, associated a varied impact on fungi, increasing relative abundance Myriangiales in young (76.37%) Capnodiales (42.37%), which improve stability health. These findings highlight effectiveness over promoting provide valuable insights for sustainable management willow plantations at stages development.

Язык: Английский

Процитировано

3

Changes in the phyllosphere and rhizosphere of a cloud forest tree fern along an elevation gradient DOI Creative Commons

Mariana Vélez-Múnera,

Morena Avitia, Eria A. Rebollar

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 15, 2024

Abstract The diversity of plant-associated microbial communities is shaped by both host factors and the environment. Natural environmental gradients, specifically elevational ones, can serve as study systems to understand community ecosystem responses changes, however relationship between elevation not completely understood, especially in non-model such wild plants. In this paper we explored role shaping structure rhizosphere phyllosphere cloud forest tree fern Cyathea fulva. Samples phyllosphere, soil were collected from 15 individual ferns across five plots along an gradient ranging 1978 2210 meters above sea level. Physicochemical data collected, with all plots. Using 16S rRNA ITS1 amplicon sequencing, tested for differences composition bacterial fungal their potential abiotic drivers. We found that alpha decreased rhizosphere, but fungi pattern was only rhizosphere. also observed significant changes Our results suggest a close overall associated ferns. envision information will help further dynamics microbiota plants, contributing conservation necessary interactions plants ecosystems wellbeing.

Язык: Английский

Процитировано

0

Composition, Seasonal Dynamics and Metabolic Potential of the Rhizosphere Microbiome Associated with Wild White Poplar DOI Creative Commons

Mikhail I. Popchenko,

Dmitry S. Karpov, Natalya S. Gladysh

и другие.

BioTech, Год журнала: 2024, Номер 13(4), С. 52 - 52

Опубликована: Дек. 1, 2024

The white poplar (Populus alba) is a dioecious woody plant with significant potential for the phytoremediation of soils. To realize this potential, it necessary to utilize growth-promoting microorganisms. One source such beneficial microorganisms rhizosphere community wild-growing trees. However, structure, dynamics, and metabolism remain poorly understood. ascertain seasonal species diversity, metabolic we sequenced 16S rRNA genes in metagenomes derived from 165 soil samples collected spring autumn root surfaces 102 trees situated disparate geographical locations. three most prevalent phyla across all are Proteobacteria, Actinobacteriota, Acidobacteriota. At order level, orders Sphingomonadales Rhizobiales. Accordingly, families Sphingomonadaceae Rhizobiaceae were identified as dominant. rhizospheric microbiome exhibited substantial inter-seasonal variation. Six families, including Caulobacteraceae, Xanthomonadaceae, Chitinophagaceae, Chthoniobacteraceae, Sphingomonadaceae, Rhizobiaceae, alterations (spring-to-autumn) locations under study. Members family, which includes nitrogen-fixing bacteria, can provide plant-available forms nitrogen nitrate ammonium. may facilitate conversion inorganic sulfur into sulfur-containing amino acids, cysteine methionine, that bioavailable plants. Furthermore, capable synthesizing organic acids (including Krebs cycle acids), some lipids sugars. Consequently, stimulate growth by providing readily available sulfur, well building blocks synthesis proteins, nucleic other macromolecules. Many these pathways, fixation, subjected changes.

Язык: Английский

Процитировано

0