Phage-encoded enzymes found in Acinetobacter baumannii convert pseudaminic acid to 8-epipseudaminic acid

Communications Biology, Год журнала: 2025, Номер 8(1)

Опубликована: Май 5, 2025

Abstract The nonulosonic acid 8-epipseudaminic was discovered only recently in two Acinetobacter baumannii strains but the genes responsible for conversion of pseudaminic to its 8-epimer were not found at K locus. Here, we use a pan-genome approach identify pair carbohydrate biosynthesis genes, epaA and epaB , demonstrate using NMR analysis capsular polysaccharide that they encode enzymes able convert 8-epimer. Via an extensive survey available A. genomes, show are present 17 different Caudoviricetes class prophages. prophages genomes carry capsule loci from isolates recovered several countries. presence produce leads modification polysaccharides decorate their cell surface with potential implications typing capsule-targeting therapies.

Язык: Английский

Structure of the K58 capsular polysaccharide produced by Acinetobacter baumannii isolate MRSN 31468 includes Pse5Ac7Ac that is 4-O-acetylated by a phage-encoded acetyltransferase

Carbohydrate Research, Год журнала: 2024, Номер 547, С. 109324 - 109324

Опубликована: Ноя. 19, 2024

Capsular polysaccharide (CPS), a heteropolymeric carbohydrate structure present on the cell surface of most isolates bacterial pathogen Acinetobacter baumannii, is major virulence determinant. Here, CPS produced by A. baumannii MRSN 31468, which carries KL58 biosynthesis locus, was studied sugar analysis, one- and two-dimensional

Язык: Английский

Capsular polysaccharide structure of Acinetobacter baumannii K58 from clinical isolate MRSN31468

Carbohydrate Research, Год журнала: 2024, Номер 546, С. 109307 - 109307

Опубликована: Ноя. 6, 2024

Язык: Английский