Abstract
Background
Plant-beneficial
bacterial
inoculants
are
of
great
interest
in
agriculture
as
they
have
the
potential
to
promote
plant
growth
and
health.
However,
inoculation
rhizosphere
microbiome
often
results
a
suboptimal
or
transient
colonization,
which
is
due
variety
factors
that
influence
fate
inoculant.
To
better
understand
plant-beneficial
complex
microbiomes,
composed
by
hundreds
genotypes
multifactorial
selection
mechanisms,
controlled
studies
with
high-complexity
soil
microbiomes
needed.
Results
We
analysed
early
compositional
changes
taxa-rich
natural
community
under
both
exponential
nutrient-rich
stationary
nutrient-limited
conditions
(i.e.
growing
stable
communities,
respectively)
following
bacterium
Pseudomonas
protegens
bulk
wheat
environment.
P.
successfully
established
all
tested
was
more
abundant
community.
Nutrient
availability
major
factor
driving
composition
structure
well
underlying
assembly
processes.
While
access
nutrients
resulted
communities
assembled
mainly
homogeneous
selection,
stochastic
processes
dominated
nutrient-deprived
conditions.
also
observed
an
increased
effect
conditions,
resulting
higher
number
amplicon
sequence
variants
(ASVs)
whose
relative
abundance
enriched.
The
produced
discrete
changes,
some
involved
other
.
Direct
competition
between
strains
partially
failed
replicate
differences
pointed
interaction
network.
Conclusions
this
study
show
nutrient
force
composition,
diversity
determines
govern
development.
successful
establishment
inoculant
facilitated
among
members
microbiome.
only
explained
indicating
indirect
interactions
spatial
distribution
interface
may
be
crucial
for
survival
certain
bacteria.
Journal of Geophysical Research Biogeosciences,
Год журнала:
2024,
Номер
129(7)
Опубликована: Июль 1, 2024
Abstract
In
the
soils
of
vanadium
(V)
smelters,
a
diverse
array
microorganisms
relies
on
metabolic
activities
for
survival
amid
stress.
However,
characteristics
and
functions
soil
microbiomes
in
V
mining
environments
remain
unexplored
continental
scale.
This
study
thoroughly
investigates
microbial
diversity,
community
assembly,
functional
potential
microbiome
across
90
smelters
China.
Alpha
diversity
decreases
significantly
along
gradient,
with
emerging
as
primary
factor
influencing
structure,
followed
by
other
environmental,
climatic,
geographic
factors.
The
null
model
reveals
that
induces
homogeneous
selection,
shaping
co‐occurrence
patterns
leading
to
increased
number
positive
associations,
particularly
keystone
genera
such
f_Gemmatimonadaceae
,
Nocardioides
Micromonospora
Rubrobacter
under
higher
concentrations
(>559.6
mg/kg).
Moreover,
metagenomic
analysis
yields
67
metagenome‐assembled
genomes,
unraveling
pathways
taxa
their
likely
involvement
V(V)
reduction
process.
Nitrate
nitrite
reductase
(
nirK
narG
),
mtrABC
are
found
be
taxonomically
affiliated
.
sp,
FEN‐1250
etc.
Additionally,
reverse
citric
acid
cycle
(rTCA)
serves
carbon
fixation
pathway,
synthesizing
alternative
energy
putative
reducers,
highlighting
potentially
synergistic
relationship
between
autotrophic
heterotrophic
processes
supports
survival.
Our
findings
comprehensively
uncover
driving
forces
behind
variation
stress,
revealing
robust
strategies
possibly
employed
indigenous
mitigate
impact
V.
These
insights
hold
applications
bioremediation.
