Trends in Microbiology, Год журнала: 2024, Номер unknown
Опубликована: Дек. 1, 2024
Язык: Английский
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Янв. 11, 2025
Abstract Seawater contains an abundance of small biomolecules, or metabolites, that are highly labile components dissolved organic matter (DOM). Marine microbes interact by exchanging thus shaping marine microbial ecology, DOM composition, and global carbon cycling. To better constrain one set microbe-metabolite interactions, we cultured the gammaproteobacterium Alteromonas macleodii MIT1002 on a range compounds excreted sympatric cyanobacterium, Prochlorococcus . could assimilate branched chain amino acids leucine, isoleucine, valine, as well 3-methyl-2-oxobutanoic acid (a ketoacid intermediate valine metabolism), but not thymidine, kynurenine, 4-hydroxybenzoic acid, other ketoacids. The assimilation indicates can metabolically process each corresponding ketoacid, suggesting transporter specificity underlies observed substrate for acid. These experiments show even subtle changes in chemical structure result different interactions fates metabolites. Significance Statement Microbial with important controls cycle. Dissolved is often considered bulk, which leaves nature these poorly constrained. Here microbe-molecule be specific, distinguishing between molecules structurally biochemically similar. This implies this pool have large impacts overall system function, measuring molecular-level resolution to characterizing interactions. We further explore mechanism underlying suggest it caused selectivity, meaning ability selectively uptake specific molecules.
Язык: Английский
Процитировано
1
Limnology and Oceanography, Год журнала: 2024, Номер 69(3), С. 449 - 466
Опубликована: Янв. 10, 2024
Abstract Organic carbon in seawater plays a significant role the global cycle. The concentration and composition of dissolved organic reflect activity biological community chemical reactions that occur seawater. From 2016 to 2019, we repeatedly sampled oligotrophic northwest Sargasso Sea vicinity Bermuda Atlantic Time‐series Study site (BATS) quantitatively follow known compounds within pool matter upper 1000 m water column. Most metabolites showed surface enrichment, 83% had significantly lower concentrations with increasing depth. Dissolved metabolite most notably revealed temporal variability. Fourteen displayed seasonality was repeated each 4 yr sampled. Concentrations vitamins, including pantothenic acid (vitamin B 5 ) riboflavin 2 ), increased annually during winter periods when mixed layer depths were deepest. During diel sampling, light‐sensitive decreased daylight hours. variability at BATS less than spatial from previous sample set collected over broad latitudinal range western Ocean. examined this study are all components central metabolism. By examining these finer resolution time‐series, begin provide insights into may be exchanged by microorganisms marine systems, data which fundamental understanding response systems future changes climate.
Язык: Английский
Процитировано
5
Nature Microbiology, Год журнала: 2024, Номер 9(8), С. 2006 - 2021
Опубликована: Июль 5, 2024
The lag phase is key in resuming bacterial growth, but it remains underexplored particularly environmental bacteria. Here we use transcriptomics and
Язык: Английский
Процитировано
4
Science Advances, Год журнала: 2025, Номер 11(1)
Опубликована: Янв. 3, 2025
Cross-feeding involves microbes consuming exudates of other surrounding microbes, mediating elemental cycling. Characterizing the diversity cross-feeding pathways in ocean illuminates evolutionary forces driving self-organization ecosystems. Here, we uncover a purine and pyrimidine network globally abundant groups. The cyanobacterium Prochlorococcus exudes both compound classes, which metabolic reconstructions suggest follows synchronous daily genome replication. Co-occurring heterotrophs differentiate into purine- pyrimidine-using generalists or specialists that use compounds for different purposes. most heterotroph, SAR11, is specialist uses purines as sources energy, carbon, and/or nitrogen, with subgroups differentiating along ocean-scale gradients supply energy turn producing putative cryptic nitrogen cycles link many microbes. Last, an SAR11 subgroup dominates where abundant, adenine additions to cultures inhibit DNA synthesis, poising cells We argue this inferred pulses from synchronize photosynthate phytoplankton.
Язык: Английский
Процитировано
0
Microbiology Spectrum, Год журнала: 2025, Номер unknown
Опубликована: Янв. 8, 2025
ABSTRACT Limnospira can colonize a wide variety of environments (e.g., freshwater, brackish, alkaline, or alkaline-saline water) and develop dominant even permanent blooms that overshadow limit the diversity adjacent phototrophs, especially in alkaline saline environments. Previous phylogenomic analysis allowed us to distinguish two major phylogenetic clades (I II) but failed clearly segregate strains according their respective habitats terms salinity biogeography. In present work, we attempted determine whether displays metabolic signatures specific its different habitats, particularly brackish ecosystems. The impact accessory gene repertoires on chemical adaptations was also determined. complement our previous investigation (Roussel et al., 2023), metabolomic 93 , grown under standardized lab culture conditions. Overall, this original work showed distinct fingerprints were correlated with biogeographic origins strains. molecules most distinguished geographic groups sugars, lipids, peptides, photosynthetic pigments, antioxidants. Interestingly, these molecular enrichments might represent consequent conditions salinity, light, oxidative stress sampling Although genes specifically involved production components remain unknown, hypothesized within extreme environments, such as those colonized by large set flexible could support peculiar metabolite sets providing remarkable local environmental IMPORTANCE are ubiquitous cyanobacteria adaptive strategies allowing them dominate range worldwide. Phylogenomic revealed We found variable portion genome be adaptation paper, displayed habitats. sought understand repertoire adaptations.
Язык: Английский
Процитировано
0
ISME Communications, Год журнала: 2024, Номер 4(1)
Опубликована: Янв. 1, 2024
is found throughout the euphotic zone in oligotrophic open ocean. Deep mixing and sinking while attached to particles can, however, transport
Язык: Английский
Процитировано
1
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Апрель 22, 2024
Abstract Limnospira can colonize a wide variety of environments ( e.g., freshwater, brackish, alkaline or alkaline-saline water) and develop dominant even permanent blooms that limit over-shadowed adjacent phototrophs diversity, especially in saline environments. Previous phylogenomic analysis allowed us to distinguish two major phylogenetic clades (I II) but failed clearly segregate strains according their respective habitats terms salinity biogeography. In the present work, we attempt determine whether displays metabolic signatures specific its different habitats, particularly brackish ecosystems, question impact accessory gene repertoires on chemical adaptations. The study metabolomic diversity 93 from Paris Museum Collection, grown under standardized lab culture conditions, showed distinct fingerprints were correlated with biogeographic origins strains. molecules most geographic groups are sugars, lipids, peptides, photosynthetic pigments, antioxidant molecules. Interestingly, these molecule enrichments might represent adaptation traits local conditions encountered sampling concerning salinity, light oxidative stress. We hypothesize within extreme environments, such as those colonized by , large set flexible genes provide remarkable environmental light, pressure). Thus, occurrence population genomes potentially involved production certain metabolites may valuable adaptative support bloom persistence beyond condition variations. Importance ubiquitous cyanobacteria able dominate range around world strategies. Phylogenomic platensis following One presume found this variable portion genome could be L. conditions. paper, alkalinesaline repertoire adaptation.
Язык: Английский
Процитировано
0
Limnology and Oceanography Methods, Год журнала: 2024, Номер 22(7), С. 451 - 463
Опубликована: Апрель 25, 2024
Abstract A targeted method for the quantification of bioavailable amide N found in marine DON (bDON) is presented. The utilizes mild acid hydrolysis to convert proteins and ‐acetyl amino polysaccharides primary amine containing products that are measured using a highly sensitive (nanomolar range precision) fluorometric technique with addition O ‐phthaldialdehyde. We find amidic bDON concentrations ranging from 0.08 1.82 μ M within waters upper 300 m southern California Current, Southern Bight, subtropical North Pacific representing 15–33% bulk concentrations. Bioassay experiments revealed consumption ~20% situ 5 days. represents simple rapid tool seawater improved analytical precision over traditional estimates
Язык: Английский
Процитировано
0
Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 69 - 99
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0
Trends in Microbiology, Год журнала: 2024, Номер unknown
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
0