The role of urinary microbiota in primary and recurrent bladder cancer: insights from a propensity score matching study
BMC Cancer,
Год журнала:
2025,
Номер
25(1)
Опубликована: Март 14, 2025
Bladder
cancer
(BCa)
is
a
common
urinary
malignancy
with
high
recurrence
rates
in
non-muscle
invasive
bladder
(NMIBC),
posing
significant
clinical
challenges.
Emerging
evidence
links
microbiota
to
progression;
however,
their
role
BCa
remains
unclear.
This
study
aimed
explore
differences
between
primary
and
recurrent
identify
potential
microbiological
markers
mechanisms
associated
recurrence.
Urine
samples
were
collected
from
170
patients,
including
125
Bca(BCa_P)
45
(BCa_R).
All
underwent
16
S
rRNA
gene
sequencing,
data
collected,
age,
sex,
body
mass
index
(BMI),
smoking
history,
pathological
grade,
other
biological
characteristics.
Propensity
score
matching
(1:1
ratio,
caliper
=
0.02)
minimized
baseline
differences,
resulting
39
matched
pairs.
Microbial
diversity
was
analyzed
using
α
β
indices.
Differential
taxa
identified
Linear
Discriminant
Analysis
Effect
Size
(LEfSe),
functional
pathways
predicted
Phylogenetic
Investigation
of
Communities
by
Reconstruction
Unobserved
States
(PICRUSt).
Alpha
significantly
higher
BCa_P
than
BCa_R,
particularly
Chao1
revealed
distinct
microbial
structures
(ADONIS,
P
0.004,
R²
0.025).
At
the
phylum
level,
both
BCa_R
dominated
Firmicutes,
Proteobacteria,
Bacteroidetes,
Actinobacteria,
Firmicutes
Bacteroidetes
lower
BCa_R.
genus
enriched
Sphingomonas,
Corynebacterium,
Capnocytophaga,
Massilia,
Aquabacterium,
while
showed
levels
Aeromonas,
Cupriavidus,
Bradyrhizobium.
Functional
predictions
glucose
metabolism
oxidative
stress
pollutant
degradation
TCA
cycle
prominent
BCa_P.
These
findings
reveal
compositions
profiles
cases
exhibiting
reduced
enrichment
potentially
pathogenic
communities,
highlighting
roles
tumor
progression
Registered
Chinese
Clinical
Trial
Registry
(ChiCTR2300070969)
on
April
27,
2023.
Язык: Английский
The inconsistent pathogenesis of endometriosis and adenomyosis: insights from endometrial metabolome and microbiome
Chao Li,
Xinxin Xu,
Xiaojie Zhao
и другие.
mSystems,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 22, 2025
ABSTRACT
Endometriosis
(EM)
and
adenomyosis
(AM)
are
interrelated
gynecological
disorders
characterized
by
the
aberrant
presence
of
endometrial
tissue
frequently
linked
with
chronic
pelvic
pain
infertility,
yet
their
pathogenetic
mechanisms
remain
largely
unclear.
In
this
cross-sectional
study,
we
analyzed
samples
from
244
participants,
split
into
91
EM
patients,
56
AM
97
healthy
controls
(HC).
We
conducted
untargeted
liquid
chromatography-mass
spectrometry
(LC-MS)
5R
16S
rRNA
sequencing
to
examine
metabolome
microbiome
profiles.
Additionally,
integrated
transcriptomic
analysis
using
nine
data
sets
investigate
biological
basis
these
conditions.
Metabolomic
profiling
revealed
distinct
metabolic
microbial
signatures.
Specific
pathways,
including
linoleic
acid
glycerophospholipid
metabolism,
show
significant
alterations
in
both
Notably,
four
metabolites,
phosphatidylcholine
40:8
[PC(40:8)],
exhibited
marked
changes
AM,
suggesting
shared
pathological
features.
Furthermore,
taxonomic
identified
unique
bacterial
species
associated
each
condition,
particularly
those
belonging
phylum
Proteobacteria,
which
correlated
altered
Machine
learning
models
demonstrated
high
predictive
accuracy
for
differentiating
between
EM,
HC
based
on
Integrative
highlighted
pathways
related
immune
response
signaling
transduction
condition.
Our
study
provides
fresh
insights
pathogenesis
through
a
multi-omic
approach,
potential
inconsistencies
underlying
mechanisms.
IMPORTANCE
Existing
research
connection
endometriosis
(AM),
underscoring
overlapping
symptoms
pathophysiological
Although
role
microbiota
inflammatory
conditions
has
been
acknowledged,
comprehensive
investigations
cases
have
limited.
Previous
studies
communities
conditions;
however,
they
were
constrained
small
sample
sizes
lack
analyses
metabolomics.
ongoing
debate
over
whether
should
be
classified
as
separate
diseases
or
phenotypes
emphasizes
necessity
further
exploration
molecular
interactions.
uncovers
signatures
revealing
that
may
contribute
pathogenesis.
integration
offers
valuable
complex
interactions
disorders.
Язык: Английский
Microbiome and metabolomic changes associated with HPV clearance in women undergoing local excisional treatment for cervical intraepithelial neoplasia
mSystems,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 20, 2025
ABSTRACT
Cervical
intraepithelial
neoplasia
(CIN)
is
a
common
gynecological
condition
often
associated
with
persistent
human
papillomavirus
(HPV)
infections.
Although
the
loop
electrosurgical
excision
procedure
(LEEP)
effective
in
removing
lesions,
some
patients
remain
HPV
positive
post-treatment.
In
this
prospective
study,
we
enrolled
reproductive-age
women
diagnosed
HPV-related
CIN
and
employed
multi-omics
analysis
of
cervicovaginal
secretion
cervical
tissue
microbiomes,
alongside
non-targeted
targeted
metabolomic
assessments.
We
aim
to
explore
role
intratissue
microbiota
metabolites
on
clearance
following
LEEP.
observed
significant
shifts
bacterial
diversity
composition
both
samples.
Notably,
distinct
species,
such
as
Lactobacillus
certain
anaerobes
(e.g.,
Prevotella
bivia
),
were
correlated
post-LEEP.
Metabolomic
profiling
revealed
that
HPV-cleared
group
exhibited
elevated
acetic
acid
levels
alterations
glycerophospholipid
metabolism,
suggesting
potential
promoting
clearance.
Correlation
analyses
demonstrated
associations
between
altered
bacteria
status,
models
incorporating
achieving
high
predictive
accuracy.
Overall,
our
findings
highlight
importance
microbiomes
facilitating
clearance,
therapeutic
targets
for
CIN.
IMPORTANCE
The
after
local
excisional
treatment
crucial
patient
health.
This
study
reveals
changes,
which
are
Through
comprehensive
approach,
identified
specific
species
metabolic
changes
correlate
successful
post-loop
procedure.
presence
beneficial
linked
metabolism
emerged
biomarkers
these
factors
play
pivotal
improving
outcomes.
These
microbiome-targeted
therapies
enhance
provide
insights
into
microbial
mechanisms
involved
Язык: Английский
LPL-RH suppresses bone loss in ovariectomised rat models
BMC Microbiology,
Год журнала:
2024,
Номер
24(1)
Опубликована: Дек. 28, 2024
Evidence
has
revealed
that
oestrogen
deprivation-induced
osteolysis
is
microbiota-dependent
and
can
be
treated
by
probiotics.
However,
the
underlying
mechanism
require
further
investigation.
This
study
aims
to
provide
additional
evidence
supporting
use
of
probiotics
as
an
adjuvant
treatment
explore
pathophysiology
oestrogen-deprived
osteolysis.
Forty-five
SD
rats
were
randomly
divided
into
five
groups
(n
=
9).
Rats
from
four
ovariectomised
with
NS,
calcium,
probiotics,
or
calcium
+
while
one
group
underwent
a
sham
operation
was
NS.
The
osteometabolic
effects
evaluated,
mechanistic
role
probiotic
supplement
explored.
Intragastric
administration
Bifidobacterium
animalis
subsp.
lactis
LPL-RH
(LPL-RH)
markedly
suppressed
osteoclastic
activation
bone
loss
downregulating
TRAP
enzymatic
activity,
OPG/RANKL
ratio,
downstream
signalling
pathway
RANKL/TRAF6/NF-κB/NFATc1/TRAP
in
rats.
also
reduced
CD4+IL-17
A+
TH17
cells
marrow,
pro-osteoclastogenic
cytokine
IL-17
A,
pro-inflammatory
molecules
(LPS),
its
binding
protein
(LBP)
blood.
restored
intestinal
ZO-1,
occludin,
claudin
2,
12,
15,
which
improved
ileal
histopathology,
oxidative
stress,
attenuated
LPS-responsive
TLR4/MyD88/NF-κB
pathway.
Furthermore,
16
S
rRNA
sequencing
altered
faecal
microbiome
reducing
relative
abundance
S24-7
at
family
level
promoting
Prevotella
Bacteroides
genus
level.
Collectively,
osteoclastogenesis
modulating
type
17
immunity
gut
microbiome.
Язык: Английский