The role of urinary microbiota in primary and recurrent bladder cancer: insights from a propensity score matching study

BMC Cancer, Год журнала: 2025, Номер 25(1)

Опубликована: Март 14, 2025

Bladder cancer (BCa) is a common urinary malignancy with high recurrence rates in non-muscle invasive bladder (NMIBC), posing significant clinical challenges. Emerging evidence links microbiota to progression; however, their role BCa remains unclear. This study aimed explore differences between primary and recurrent identify potential microbiological markers mechanisms associated recurrence. Urine samples were collected from 170 patients, including 125 Bca(BCa_P) 45 (BCa_R). All underwent 16 S rRNA gene sequencing, data collected, age, sex, body mass index (BMI), smoking history, pathological grade, other biological characteristics. Propensity score matching (1:1 ratio, caliper = 0.02) minimized baseline differences, resulting 39 matched pairs. Microbial diversity was analyzed using α β indices. Differential taxa identified Linear Discriminant Analysis Effect Size (LEfSe), functional pathways predicted Phylogenetic Investigation of Communities by Reconstruction Unobserved States (PICRUSt). Alpha significantly higher BCa_P than BCa_R, particularly Chao1 revealed distinct microbial structures (ADONIS, P 0.004, R² 0.025). At the phylum level, both BCa_R dominated Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes Bacteroidetes lower BCa_R. genus enriched Sphingomonas, Corynebacterium, Capnocytophaga, Massilia, Aquabacterium, while showed levels Aeromonas, Cupriavidus, Bradyrhizobium. Functional predictions glucose metabolism oxidative stress pollutant degradation TCA cycle prominent BCa_P. These findings reveal compositions profiles cases exhibiting reduced enrichment potentially pathogenic communities, highlighting roles tumor progression Registered Chinese Clinical Trial Registry (ChiCTR2300070969) on April 27, 2023.

Язык: Английский

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The inconsistent pathogenesis of endometriosis and adenomyosis: insights from endometrial metabolome and microbiome

mSystems, Год журнала: 2025, Номер unknown

Опубликована: Апрель 22, 2025

ABSTRACT Endometriosis (EM) and adenomyosis (AM) are interrelated gynecological disorders characterized by the aberrant presence of endometrial tissue frequently linked with chronic pelvic pain infertility, yet their pathogenetic mechanisms remain largely unclear. In this cross-sectional study, we analyzed samples from 244 participants, split into 91 EM patients, 56 AM 97 healthy controls (HC). We conducted untargeted liquid chromatography-mass spectrometry (LC-MS) 5R 16S rRNA sequencing to examine metabolome microbiome profiles. Additionally, integrated transcriptomic analysis using nine data sets investigate biological basis these conditions. Metabolomic profiling revealed distinct metabolic microbial signatures. Specific pathways, including linoleic acid glycerophospholipid metabolism, show significant alterations in both Notably, four metabolites, phosphatidylcholine 40:8 [PC(40:8)], exhibited marked changes AM, suggesting shared pathological features. Furthermore, taxonomic identified unique bacterial species associated each condition, particularly those belonging phylum Proteobacteria, which correlated altered Machine learning models demonstrated high predictive accuracy for differentiating between EM, HC based on Integrative highlighted pathways related immune response signaling transduction condition. Our study provides fresh insights pathogenesis through a multi-omic approach, potential inconsistencies underlying mechanisms. IMPORTANCE Existing research connection endometriosis (AM), underscoring overlapping symptoms pathophysiological Although role microbiota inflammatory conditions has been acknowledged, comprehensive investigations cases have limited. Previous studies communities conditions; however, they were constrained small sample sizes lack analyses metabolomics. ongoing debate over whether should be classified as separate diseases or phenotypes emphasizes necessity further exploration molecular interactions. uncovers signatures revealing that may contribute pathogenesis. integration offers valuable complex interactions disorders.

Язык: Английский

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Microbiome and metabolomic changes associated with HPV clearance in women undergoing local excisional treatment for cervical intraepithelial neoplasia

mSystems, Год журнала: 2025, Номер unknown

Опубликована: Май 20, 2025

ABSTRACT Cervical intraepithelial neoplasia (CIN) is a common gynecological condition often associated with persistent human papillomavirus (HPV) infections. Although the loop electrosurgical excision procedure (LEEP) effective in removing lesions, some patients remain HPV positive post-treatment. In this prospective study, we enrolled reproductive-age women diagnosed HPV-related CIN and employed multi-omics analysis of cervicovaginal secretion cervical tissue microbiomes, alongside non-targeted targeted metabolomic assessments. We aim to explore role intratissue microbiota metabolites on clearance following LEEP. observed significant shifts bacterial diversity composition both samples. Notably, distinct species, such as Lactobacillus certain anaerobes (e.g., Prevotella bivia ), were correlated post-LEEP. Metabolomic profiling revealed that HPV-cleared group exhibited elevated acetic acid levels alterations glycerophospholipid metabolism, suggesting potential promoting clearance. Correlation analyses demonstrated associations between altered bacteria status, models incorporating achieving high predictive accuracy. Overall, our findings highlight importance microbiomes facilitating clearance, therapeutic targets for CIN. IMPORTANCE The after local excisional treatment crucial patient health. This study reveals changes, which are Through comprehensive approach, identified specific species metabolic changes correlate successful post-loop procedure. presence beneficial linked metabolism emerged biomarkers these factors play pivotal improving outcomes. These microbiome-targeted therapies enhance provide insights into microbial mechanisms involved

Язык: Английский

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LPL-RH suppresses bone loss in ovariectomised rat models

BMC Microbiology, Год журнала: 2024, Номер 24(1)

Опубликована: Дек. 28, 2024

Evidence has revealed that oestrogen deprivation-induced osteolysis is microbiota-dependent and can be treated by probiotics. However, the underlying mechanism require further investigation. This study aims to provide additional evidence supporting use of probiotics as an adjuvant treatment explore pathophysiology oestrogen-deprived osteolysis. Forty-five SD rats were randomly divided into five groups (n = 9). Rats from four ovariectomised with NS, calcium, probiotics, or calcium + while one group underwent a sham operation was NS. The osteometabolic effects evaluated, mechanistic role probiotic supplement explored. Intragastric administration Bifidobacterium animalis subsp. lactis LPL-RH (LPL-RH) markedly suppressed osteoclastic activation bone loss downregulating TRAP enzymatic activity, OPG/RANKL ratio, downstream signalling pathway RANKL/TRAF6/NF-κB/NFATc1/TRAP in rats. also reduced CD4+IL-17 A+ TH17 cells marrow, pro-osteoclastogenic cytokine IL-17 A, pro-inflammatory molecules (LPS), its binding protein (LBP) blood. restored intestinal ZO-1, occludin, claudin 2, 12, 15, which improved ileal histopathology, oxidative stress, attenuated LPS-responsive TLR4/MyD88/NF-κB pathway. Furthermore, 16 S rRNA sequencing altered faecal microbiome reducing relative abundance S24-7 at family level promoting Prevotella Bacteroides genus level. Collectively, osteoclastogenesis modulating type 17 immunity gut microbiome.

Язык: Английский

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