Cell Reports,
Год журнала:
2024,
Номер
43(7), С. 114408 - 114408
Опубликована: Июнь 26, 2024
Honeybees
are
important
pollinators
worldwide,
with
their
gut
microbiota
playing
a
crucial
role
in
maintaining
health.
The
bacteria
of
honeybees
consist
primarily
five
core
lineages
that
spread
through
social
interactions.
Previous
studies
have
provided
basic
understanding
the
composition
and
function
honeybee
microbiota,
recent
advancements
focusing
on
analyzing
diversity
at
strain
level
changes
bacterial
functional
genes.
Research
across
different
regions
globally
has
insights
into
microbial
ecology.
Additionally,
findings
shed
light
mechanisms
host
specificity
bacteria.
This
review
explores
temporospatial
dynamics
discussing
reasons
behind
these
fluctuations.
synopsis
provides
host-microbe
interactions
is
invaluable
for
Proceedings of the National Academy of Sciences,
Год журнала:
2023,
Номер
120(33)
Опубликована: Авг. 9, 2023
Host
specificity
is
observed
in
gut
symbionts
of
diverse
animal
lineages.
But
how
hosts
maintain
while
rejecting
their
close
relatives
remains
elusive.
We
use
eusocial
bees
and
codiversified
bacteria
to
understand
host
regulation
driving
symbiotic
specificity.
The
cross-inoculation
bumblebee
Gilliamella
induced
higher
prostaglandin
the
honeybee
gut,
promoting
a
pronounced
response
through
immune
deficiency
(IMD)
Toll
pathways.
Gene
silencing
vitamin
C
treatments
indicate
that
reactive
oxygen
species
(ROS),
not
antimicrobial
peptides,
acts
as
effector
inhibiting
non-native
strain.
Quantitative
PCR
RNAi
further
reveal
regulatory
function
IMD
pathways,
which
Relish
dorsal-1
may
regulate
Dual
Oxidase
(
Duox
)
for
ROS
production.
Therefore,
maintains
by
creating
hostile
environment
exotic
bacteria,
differential
its
system,
reflecting
co-opting
existing
machinery
evolved
combat
pathogens.
The ISME Journal,
Год журнала:
2023,
Номер
17(9), С. 1382 - 1395
Опубликована: Июнь 14, 2023
Managed
honey
bee
(Apis
mellifera)
populations
play
a
crucial
role
in
supporting
pollination
of
food
crops
but
are
facing
unsustainable
colony
losses,
largely
due
to
rampant
disease
spread
within
agricultural
environments.
While
mounting
evidence
suggests
that
select
lactobacilli
strains
(some
being
natural
symbionts
bees)
can
protect
against
multiple
infections,
there
has
been
limited
validation
at
the
field-level
and
few
methods
exist
for
applying
viable
microorganisms
hive.
Here,
we
compare
how
two
different
delivery
systems-standard
pollen
patty
infusion
novel
spray-based
formulation-affect
supplementation
three-strain
consortium
(LX3).
Hives
pathogen-dense
region
California
supplemented
4
weeks
then
monitored
over
20-week
period
health
outcomes.
Results
show
both
facilitate
uptake
LX3
adult
bees,
although
do
not
colonize
long-term.
Despite
this,
treatments
induce
transcriptional
immune
responses
leading
sustained
decreases
many
opportunistic
bacterial
fungal
pathogens,
as
well
selective
enrichment
core
including
Bombilactobacillus,
Bifidobacterium,
Lactobacillus,
Bartonella
spp.
These
changes
ultimately
associated
with
greater
brood
production
growth
relative
vehicle
controls,
no
apparent
trade-offs
ectoparasitic
Varroa
mite
burdens.
Furthermore,
spray-LX3
exerts
potent
activities
Ascosphaera
apis
(a
deadly
pathogen)
likely
stemming
from
in-hive
dispersal
differences,
whereas
patty-LX3
promotes
synergistic
development
via
unique
nutritional
benefits.
findings
provide
foundational
basis
probiotic
application
apiculture
collectively
highlight
importance
considering
method
management
strategies.
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Май 20, 2023
Abstract
Nosema
ceranae
is
an
intracellular
parasite
invading
the
midgut
of
honeybees,
which
causes
serious
nosemosis
implicated
in
honeybee
colony
losses
worldwide.
The
core
gut
microbiota
involved
protecting
against
parasitism,
and
genetically
engineering
native
symbionts
provides
a
novel
efficient
way
to
fight
pathogens.
Here,
using
laboratory-generated
bees
mono-associated
with
members,
we
find
that
Snodgrassella
alvi
inhibit
microsporidia
proliferation,
potentially
via
stimulation
host
oxidant-mediated
immune
response.
Accordingly,
N.
employs
thioredoxin
glutathione
systems
defend
oxidative
stress
maintain
balanced
redox
equilibrium,
essential
for
infection
process.
We
knock
down
gene
expression
nanoparticle-mediated
RNA
interference,
targets
γ-glutamyl-cysteine
synthetase
reductase
genes
microsporidia.
It
significantly
reduces
spore
load,
confirming
importance
antioxidant
mechanism
invasion
parasite.
Finally,
modify
symbiotic
S.
deliver
dsRNA
corresponding
system
engineered
induces
interference
represses
expression,
thereby
inhibits
parasitism
significantly.
Specifically,
most
suppressed
by
recombinant
strain
or
mixture
bacteria
expressing
variable
dsRNA.
Our
findings
extend
our
previous
understanding
protection
provide
symbiont-mediated
RNAi
inhibiting
honeybees.
