Regulatory effects on virulence and phage susceptibility revealed by sdiA mutation in Klebsiella pneumoniae DOI Creative Commons
Sergio Silva-Bea,

Pablo Maseda,

Ana Otero

и другие.

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Март 13, 2025

Introduction The World Health Organization has identified multi-drug resistant Klebsiella pneumoniae strains as the highest priority in 2024. Understanding regulatory routes of virulence features is crucial for development novel anti-virulence strategies. SdiA, a LuxR-like quorum sensing (QS) receptor that responds to N- acyl-homoserine lactones (AHLs), involved regulation traits some Gram-negative bacteria. function this K. remains uncertain. objective present study was elucidate SdiA biofilm formation and virulence. Methods To end, genetic knockout sdiA conducted, virulence-related phenotypic studies were performed following AHL provision. Results Discussion results demonstrate deficiency increases susceptibility phage infection human serum resistance, promotes maturation cell filamentation, although no effect on observed vivo Galleria mellonella model. On other hand, C6-HSL promoted -dependent maturation, capsule production resistance while reducing against G. absence . addition did not affect susceptibility. AHLs exert dual influence phenotypes, operating both independently hierarchically. These findings provide new insights into its by SdiA.

Язык: Английский

Chalcone derivative enhance poultry meat preservation through quorum sensing inhibition against Salmonella (Salmonella enterica serovar Typhi) contamination DOI
S.P. Ramya Ranjan Nayak,

Pratik Pohokar,

Anamika Das

и другие.

Food Control, Год журнала: 2025, Номер unknown, С. 111155 - 111155

Опубликована: Янв. 1, 2025

Процитировано

0
Regulatory effects on virulence and phage susceptibility revealed by sdiA mutation in Klebsiella pneumoniae DOI Creative Commons
Sergio Silva-Bea,

Pablo Maseda,

Ana Otero

и другие.

Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15

Опубликована: Март 13, 2025

Introduction The World Health Organization has identified multi-drug resistant Klebsiella pneumoniae strains as the highest priority in 2024. Understanding regulatory routes of virulence features is crucial for development novel anti-virulence strategies. SdiA, a LuxR-like quorum sensing (QS) receptor that responds to N- acyl-homoserine lactones (AHLs), involved regulation traits some Gram-negative bacteria. function this K. remains uncertain. objective present study was elucidate SdiA biofilm formation and virulence. Methods To end, genetic knockout sdiA conducted, virulence-related phenotypic studies were performed following AHL provision. Results Discussion results demonstrate deficiency increases susceptibility phage infection human serum resistance, promotes maturation cell filamentation, although no effect on observed vivo Galleria mellonella model. On other hand, C6-HSL promoted -dependent maturation, capsule production resistance while reducing against G. absence . addition did not affect susceptibility. AHLs exert dual influence phenotypes, operating both independently hierarchically. These findings provide new insights into its by SdiA.

Язык: Английский

Процитировано

0