Viruses, Год журнала: 2024, Номер 16(11), С. 1717 - 1717
Опубликована: Окт. 31, 2024
Increasing evidence suggests bats are the ancestral hosts of majority coronaviruses. In general, coronaviruses primarily target gastrointestinal system, while some strains, especially Betacoronaviruses with most relevant representatives SARS-CoV, MERS-CoV, and SARS-CoV-2, also cause severe respiratory disease in humans other mammals. We previously reported susceptibility
Язык: Английский
Microorganisms, Год журнала: 2025, Номер 13(3), С. 572 - 572
Опубликована: Март 3, 2025
Lower respiratory infections, mostly caused by viral or bacterial pathogens, remain a leading global cause of mortality. The differences between animal models and humans contribute to inefficiencies in drug development, highlighting the need for more relevant predictive, non-animal models. In this context, AlveolAir™, fully primary vitro 3D human alveolar model, was characterized demonstrated sustained presence type I (ATI) II (ATII) cells. This model exhibited functional barrier over 30-day period, evidenced high transepithelial electrical resistance (TEER). These findings were further validated tight junctions’ confocal microscopy low permeability Lucifer yellow, confirming AlveolAir™ as robust platform transport assays. Additionally, successful infections with H1N1 SARS-CoV-2 viruses achieved, antiviral treatments Baloxavir Remdesivir, respectively, effectively reduced replication. Interestingly, both infected only epithelial layer without replicating endothelial indicate assessing toxicity xenobiotics evaluating efficacy novel therapies.
Язык: Английский
Процитировано
0
Journal of General Virology, Год журнала: 2025, Номер 106(4)
Опубликована: Апрель 4, 2025
Aminopeptidase N (APN) is a transmembrane protein that mediates the attachment of spike several clinically important coronaviruses (CoVs) responsible for respiratory and intestinal diseases in animals humans. To assess potential APN-mediated viral tropism, we characterized APN receptor distribution tissues various artiodactyls (cervids, bovids, camelids suids) carnivores (canids, felids, mustelids phocids) using immunohistochemistry. In lungs, expression was limited to artiodactyls, with strong bronchiolar epithelium weaker pneumocytes. Nasal turbinate tracheal samples, where available, showed stronger over carnivores. consistently detected on microvilli enterocytes small intestine across multiple taxa, while presence colon more variable. Of examined, pig alpaca expressed most abundant upper lower tract. silico evaluation orthologue sequences from humans, identified distinct evolutionary relationships. Further binding predictions alphacoronavirus human coronavirus 229E cognate heterologous revealed substantial overlapping footprints high conservation amino acid residues, suggesting an divergence subsequent adaptation 229E-like or ancestral virus within non-human animal host. This combined anatomical approach enhances understanding host susceptibility, tissue tropism transmission mechanisms APN-dependent CoVs has inform future strategies disease modelling, surveillance control.
Язык: Английский
Процитировано
0
Viruses, Год журнала: 2024, Номер 16(6), С. 984 - 984
Опубликована: Июнь 19, 2024
Viral tropism is most commonly linked to receptor use, but host cell protease use can be a notable factor in susceptibility infection. Here we review the of proteases by human viruses, focusing on those with primarily respiratory tropism, particularly SARS-CoV-2. We first describe various classes present tract, as well elsewhere body, and incorporate targeting these therapeutic drugs for humans. Host are also systemic spread viruses play important roles outside tract; therefore, address how affect across spectrum infections that occur humans, intending understand extrapulmonary
Язык: Английский
Процитировано
2
Cureus, Год журнала: 2024, Номер unknown
Опубликована: Май 15, 2024
The COVID-19 pandemic caused by the coronavirus SARS-CoV-2 revealed a huge number of problems as well discoveries in medicine, notably, regarding effects virus on central nervous system (CNS) and peripheral (PNS). This paper is narrative review that takes deep dive into complex interactions between NS. Therefore, this explains broad range neurological manifestations neurodegenerative diseases virus. It carefully considers routes through which reaches NS, including olfactory course, hematogenous route, are also covered when discussing virus's direct indirect mechanisms neuropathogenesis. Besides pathologies such stroke, encephalitis, Guillain-Barré syndrome, Parkinson's disease, multiple sclerosis, focus area given to challenges making diagnosis, treatment, management these conditions during pandemic. examines strategic interventional approaches utilized prevent disorders, ACE2 receptors implicated mediation COVID-19. detailed overview, combines research outputs with case data, directed at tackling challenge, view toward better patient care outcomes future.
Язык: Английский
Процитировано
2
One Health Outlook, Год журнала: 2024, Номер 6(1)
Опубликована: Сен. 1, 2024
Abstract Background Human activities including deforestation, urbanization, and wildlife exploitation increase the risk of transmission zoonotic diseases. Urban peri-urban species often flourish in human-altered environments, with their survival behavior heavily influenced by human-generated food waste. In Catalonia, Spain, other Mediterranean regions, rodents, house mouse ( Mus musculus) , black rat Rattus rattus) Norway norvegicus) as well wild boar Sus scrofa ) are common urban areas. These host numerous infectious agents, coronaviruses (CoVs), posing potential human health risks. During coronavirus disease 2019 (COVID-19) pandemic, severe acute respiratory syndrome 2 (SARS-CoV-2) evolved to infect previously non-susceptible species, variants capable infecting emphasizing importance surveillance studies. Methods The present study assessed SARS-CoV-2 presence and/or exposure 232 313 boar, 37 Vietnamese Pot-bellied pigs Catalonia during pandemic period (2020–2023). Results All animals tested for infection (232 rodents 29 boar) were negative. For exposure, 3 out (0.96%) positive ELISA, while remaining 32 310 all Cross-reactivity CoVs was predicted ELISA-positive samples, negative virus neutralization assay, considered gold standard technique. Conclusions absence or rodent supports negligible role viral spread COVID-19 Catalonia. However, proximity humans ongoing genetic evolution underline need continued monitoring. Surveillance animal can contribute design measures control emergence new reservoirs intermediate hosts that could facilitate spillover events.
Язык: Английский
Процитировано
0
Viruses, Год журнала: 2024, Номер 16(11), С. 1717 - 1717
Опубликована: Окт. 31, 2024
Increasing evidence suggests bats are the ancestral hosts of majority coronaviruses. In general, coronaviruses primarily target gastrointestinal system, while some strains, especially Betacoronaviruses with most relevant representatives SARS-CoV, MERS-CoV, and SARS-CoV-2, also cause severe respiratory disease in humans other mammals. We previously reported susceptibility
Язык: Английский
Процитировано
0