The transcription factor STE12 influences growth on several carbon sources and production of dehydroacetic acid (DHAA) in Trichoderma reesei DOI Creative Commons
Miriam Schalamun, Wolfgang Hinterdobler, Johann Schinnerl

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Янв. 11, 2024

Abstract The filamentous ascomycete Trichoderma reesei, known for its prolific cellulolytic enzyme production, recently also gained attention secondary metabolite synthesis. Both processes are intricately influenced by environmental factors like carbon source availability and light exposure. Here, we explore the role of transcription factor STE12 in regulating metabolic pathways T. reesei terms gene regulation, utilization biosynthesis metabolites. We show that is involved cellulase expression growth on sources associated with iron homeostasis. impacts regulation a dependent manner cellulose modulation several CAZyme encoding genes as well metabolism. selectively influences sorbicillinoid trichodimerol, while not affecting bisorbibutenolide, which was shown to be regulated MAPkinase pathway upstream signaling cascade. further report dehydroacetic acid (DHAA) compound antimicrobial properties, subject STE12. conclude, exerts functions beyond development hence contributes balance energy distribution between substrate consumption, reproduction defense.

Язык: Английский

p21-activated kinase is involved in the sporulation, pathogenicity, and stress response of Arthrobotrys oligospora under the indirect regulation of Rho GTPase-activating protein DOI Creative Commons
Meichen Zhu, Yankun Liu, Xuewei Yang

и другие.

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Сен. 14, 2023

The p21-GTPase-activated protein kinases (PAKs) participate in signal transduction downstream of Rho GTPases, which are regulated by GTPase-activating proteins (Rho-GAP). Herein, we characterized two orthologous Rho-GAPs (AoRga1 and AoRga2) PAKs (AoPak1 AoPak2) through bioinformatics analysis reverse genetics Arthrobotrys oligospora, a typical nematode-trapping (NT) fungus. transcription analyses performed at different development stages suggested that Aopaks Aorga1 play crucial role during sporulation trap formation, respectively. In addition, successfully deleted Aopak1 via the homologous recombination method. disruption caused remarkable reduction spore yield number nuclei per cell, but did not affect mycelial growth. ∆Aopak1 mutants, was decreased 48 h after introduction nematodes, nematode predatory efficiency affected because extracellular proteolytic activity increased. On contrary, traps ∆Aorga1 mutants significantly increased 36 h. had effects on sensitivity to cell-wall-disturbing reagent oxidant. A yeast two-hybrid assay revealed AoPak1 AoRga1 both interacted with AoRac, also AoCdc42. Furthermore, were up-regulated down-regulated mutants. These results reveal indirectly AoPAKs regulating small GTPases.

Язык: Английский

Процитировано

3
Involvement of AoMdr1 in the Regulation of the Fluconazole Resistance, Mycelial Fusion, Conidiation, and Trap Formation of Arthrobotrys oligospora DOI Creative Commons
Yankun Liu, Xuewei Yang, Meichen Zhu

и другие.

Microorganisms, Год журнала: 2023, Номер 11(6), С. 1612 - 1612

Опубликована: Июнь 19, 2023

Multidrug resistance (Mdr) proteins are critical for maintenance of drug in fungi. Mdr1 has been extensively studied Candida albicans; its role other fungi is largely unknown. In this study, we identified a homologous protein Mdr (AoMdr1) the nematode-trapping (NT) fungus Arthrobotrys oligospora. It was found that deletion Aomdr1 resulted significant reduction number hyphal septa and nuclei as well increased sensitivity to fluconazole hyperosmotic stress SDS. The also led remarkable increase numbers traps mycelial loops traps. Notably, AoMdr1 able regulate fusion under low-nutrient conditions, but not nutrient-rich conditions. involved secondary metabolism, caused an arthrobotrisins (specific compounds produced by NT fungi). These results suggest plays crucial resistance, fusion, conidiation, trap formation, metabolism A. Our study contributes understanding growth development

Язык: Английский

Процитировано

1
Characterization of Genome-wide Phylogenetic Conflict Uncovers Evolutionary Modes of Carnivorous Fungi DOI Creative Commons
Weiwei Zhang, Yani Fan, Wei Deng

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 25, 2024

Abstract Mass extinction has often paved the way for rapid evolutionary radiation, resulting in emergence of diverse taxa within specific lineages. While and diversification carnivorous nematode-trapping fungi (NTF) Ascomycota been linked to Permian-Triassic (PT) extinction, processes underlying NTF radiation remain unclear. Here, we conducted phylogenomic analyses using 23 genomes spanning three lineages, each employing distinct nematode traps — mechanical ( Drechslerella spp.), three-dimensional (3-D) adhesive Arthrobotrys two-dimensional (2-D) Dactylellina one non-NTF species as outgroup. This analysis revealed how mechanisms contributed tempo evolution radiation. The genome-scale tree NTFs suggested that emerged earlier than . Extensive genome-wide phylogenetic discordance was observed, mainly due incomplete lineage sorting (ILS) between lineages (∼81.3%). Modes non-vertical (i.e., introgression horizontal gene transfer) also discordance. ILS genes are associated with hyphal growth trap morphogenesis (e.g., those cell membrane system cellular polarity division) exhibited signs positive selection.

Язык: Английский

Процитировано

0
Characterization of genome-wide phylogenetic conflict uncovers evolutionary modes of carnivorous fungi DOI Creative Commons
Weiwei Zhang, Yani Fan, Wei Deng

и другие.

mBio, Год журнала: 2024, Номер 15(10)

Опубликована: Авг. 29, 2024

Mass extinction has often paved the way for rapid evolutionary radiation, resulting in emergence of diverse taxa within specific lineages. The and diversification carnivorous nematode-trapping fungi (NTF) Ascomycota have been linked to Permian-Triassic (PT) extinction, but processes underlying NTF radiation remain unclear. We conducted phylogenomic analyses using 23 genomes that represent three lineages, each employing distinct nematode traps-mechanical traps (

Язык: Английский

Процитировано

0
The SUMO gene MrSmt3 is involved in SUMOylation, conidiation and stress response in Metarhizium robertsii DOI Creative Commons

Jueping Song,

Hanyuan Chen,

D. X. Xie

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Сен. 27, 2024

Язык: Английский

Процитировано

0
Identification of Homeobox Transcription Factors in a Dimorphic Fungus Talaromyces marneffei and Protein-Protein Interaction Prediction of RfeB DOI Creative Commons
Monsicha Pongpom, Nopawit Khamto,

Panwarit Sukantamala

и другие.

Journal of Fungi, Год журнала: 2024, Номер 10(10), С. 687 - 687

Опубликована: Сен. 30, 2024

Talaromyces marneffei is a thermally dimorphic fungus that can cause life-threatening systemic mycoses, particularly in immunocompromised individuals. Fungal homeobox transcription factors control various developmental processes, including the regulation of sexual reproduction, morphology, metabolism, and virulence. However, function proteins T. has not been fully explored. Here, we searched genome for total predicted their biological relevance by performing gene expression analysis different cell types, conidia, mycelia, yeasts, during phase transition. RfeB selected further computational since (i) its transcripts were differentially expressed phases marneffei, (ii) this protein contains highly conserved protein-protein interaction region (IR), which could be important pathobiology have therapeutic application. To assess structure-function IR region, silico alanine substitutions performed at three-conserved residues (Asp276, Glu279, Gln282) RfeB, generating triple mutated protein. Using 3D modeling molecular dynamics simulations, compared complex formation wild-type with putative partner candidate TmSwi5. Our results demonstrated exhibited increased free binding energy, elevated compactness, reduced number atomic contacts, suggesting disrupted stability interaction. Notably, our model revealed primarily stabilized sites located central (CR). This approach mutagenesis provide foundation future experimental studies on functional characterization other homeodomain-containing marneffei.

Язык: Английский

Процитировано

0
The transcription factor STE12 influences growth on several carbon sources and production of dehydroacetic acid (DHAA) in Trichoderma reesei DOI Creative Commons
Miriam Schalamun, Wolfgang Hinterdobler, Johann Schinnerl

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Янв. 11, 2024

Abstract The filamentous ascomycete Trichoderma reesei, known for its prolific cellulolytic enzyme production, recently also gained attention secondary metabolite synthesis. Both processes are intricately influenced by environmental factors like carbon source availability and light exposure. Here, we explore the role of transcription factor STE12 in regulating metabolic pathways T. reesei terms gene regulation, utilization biosynthesis metabolites. We show that is involved cellulase expression growth on sources associated with iron homeostasis. impacts regulation a dependent manner cellulose modulation several CAZyme encoding genes as well metabolism. selectively influences sorbicillinoid trichodimerol, while not affecting bisorbibutenolide, which was shown to be regulated MAPkinase pathway upstream signaling cascade. further report dehydroacetic acid (DHAA) compound antimicrobial properties, subject STE12. conclude, exerts functions beyond development hence contributes balance energy distribution between substrate consumption, reproduction defense.

Язык: Английский

Процитировано

0