
Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Янв. 11, 2024
Язык: Английский
Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Янв. 11, 2024
Язык: Английский
Frontiers in Microbiology, Год журнала: 2023, Номер 14
Опубликована: Сен. 14, 2023
The p21-GTPase-activated protein kinases (PAKs) participate in signal transduction downstream of Rho GTPases, which are regulated by GTPase-activating proteins (Rho-GAP). Herein, we characterized two orthologous Rho-GAPs (AoRga1 and AoRga2) PAKs (AoPak1 AoPak2) through bioinformatics analysis reverse genetics Arthrobotrys oligospora, a typical nematode-trapping (NT) fungus. transcription analyses performed at different development stages suggested that Aopaks Aorga1 play crucial role during sporulation trap formation, respectively. In addition, successfully deleted Aopak1 via the homologous recombination method. disruption caused remarkable reduction spore yield number nuclei per cell, but did not affect mycelial growth. ∆Aopak1 mutants, was decreased 48 h after introduction nematodes, nematode predatory efficiency affected because extracellular proteolytic activity increased. On contrary, traps ∆Aorga1 mutants significantly increased 36 h. had effects on sensitivity to cell-wall-disturbing reagent oxidant. A yeast two-hybrid assay revealed AoPak1 AoRga1 both interacted with AoRac, also AoCdc42. Furthermore, were up-regulated down-regulated mutants. These results reveal indirectly AoPAKs regulating small GTPases.
Язык: Английский
Процитировано
3Microorganisms, Год журнала: 2023, Номер 11(6), С. 1612 - 1612
Опубликована: Июнь 19, 2023
Multidrug resistance (Mdr) proteins are critical for maintenance of drug in fungi. Mdr1 has been extensively studied Candida albicans; its role other fungi is largely unknown. In this study, we identified a homologous protein Mdr (AoMdr1) the nematode-trapping (NT) fungus Arthrobotrys oligospora. It was found that deletion Aomdr1 resulted significant reduction number hyphal septa and nuclei as well increased sensitivity to fluconazole hyperosmotic stress SDS. The also led remarkable increase numbers traps mycelial loops traps. Notably, AoMdr1 able regulate fusion under low-nutrient conditions, but not nutrient-rich conditions. involved secondary metabolism, caused an arthrobotrisins (specific compounds produced by NT fungi). These results suggest plays crucial resistance, fusion, conidiation, trap formation, metabolism A. Our study contributes understanding growth development
Язык: Английский
Процитировано
1bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Март 25, 2024
Abstract Mass extinction has often paved the way for rapid evolutionary radiation, resulting in emergence of diverse taxa within specific lineages. While and diversification carnivorous nematode-trapping fungi (NTF) Ascomycota been linked to Permian-Triassic (PT) extinction, processes underlying NTF radiation remain unclear. Here, we conducted phylogenomic analyses using 23 genomes spanning three lineages, each employing distinct nematode traps — mechanical ( Drechslerella spp.), three-dimensional (3-D) adhesive Arthrobotrys two-dimensional (2-D) Dactylellina one non-NTF species as outgroup. This analysis revealed how mechanisms contributed tempo evolution radiation. The genome-scale tree NTFs suggested that emerged earlier than . Extensive genome-wide phylogenetic discordance was observed, mainly due incomplete lineage sorting (ILS) between lineages (∼81.3%). Modes non-vertical (i.e., introgression horizontal gene transfer) also discordance. ILS genes are associated with hyphal growth trap morphogenesis (e.g., those cell membrane system cellular polarity division) exhibited signs positive selection.
Язык: Английский
Процитировано
0mBio, Год журнала: 2024, Номер 15(10)
Опубликована: Авг. 29, 2024
Mass extinction has often paved the way for rapid evolutionary radiation, resulting in emergence of diverse taxa within specific lineages. The and diversification carnivorous nematode-trapping fungi (NTF) Ascomycota have been linked to Permian-Triassic (PT) extinction, but processes underlying NTF radiation remain unclear. We conducted phylogenomic analyses using 23 genomes that represent three lineages, each employing distinct nematode traps-mechanical traps (
Язык: Английский
Процитировано
0Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Сен. 27, 2024
Язык: Английский
Процитировано
0Journal of Fungi, Год журнала: 2024, Номер 10(10), С. 687 - 687
Опубликована: Сен. 30, 2024
Talaromyces marneffei is a thermally dimorphic fungus that can cause life-threatening systemic mycoses, particularly in immunocompromised individuals. Fungal homeobox transcription factors control various developmental processes, including the regulation of sexual reproduction, morphology, metabolism, and virulence. However, function proteins T. has not been fully explored. Here, we searched genome for total predicted their biological relevance by performing gene expression analysis different cell types, conidia, mycelia, yeasts, during phase transition. RfeB selected further computational since (i) its transcripts were differentially expressed phases marneffei, (ii) this protein contains highly conserved protein-protein interaction region (IR), which could be important pathobiology have therapeutic application. To assess structure-function IR region, silico alanine substitutions performed at three-conserved residues (Asp276, Glu279, Gln282) RfeB, generating triple mutated protein. Using 3D modeling molecular dynamics simulations, compared complex formation wild-type with putative partner candidate TmSwi5. Our results demonstrated exhibited increased free binding energy, elevated compactness, reduced number atomic contacts, suggesting disrupted stability interaction. Notably, our model revealed primarily stabilized sites located central (CR). This approach mutagenesis provide foundation future experimental studies on functional characterization other homeodomain-containing marneffei.
Язык: Английский
Процитировано
0Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Янв. 11, 2024
Язык: Английский
Процитировано
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