Mobile genetic elements encoding antibiotic resistance genes and virulence genes in Klebsiella pneumoniae: important pathways for the acquisition of virulence and resistance
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 24, 2025
Klebsiella
pneumoniae
is
an
opportunistic
pathogen
primarily
associated
with
nosocomial
infections,
characterized
by
a
propensity
for
multi-drug
resistance
and
the
potential
evolution
into
hypervirulent
strains.
Based
on
its
phenotypic
genotypic
characteristics,
K.
can
be
classified
two
types:
classical
(cKP)
(hvKP).
The
spread
of
mobile
genetic
elements
(MGEs)
in
has
led
to
emergence
carbapenem-resistant
(CRKP)
(CR-hvKP).
CR-hvKP
particularly
concerning
due
multidrug
resistance,
high
pathogenicity,
increased
transmissibility.
This
review
summarizes
types
MGEs
present
pneumoniae,
mechanisms
horizontal
gene
transfer
(HGT)
mediated
these
elements,
their
roles
dissemination
antibiotic
genes
(ARGs)
virulence
genes,
relationships
among
that
resemble
Russian
dolls
or
exhibit
hybrid
characteristics.
Additionally,
clinical
treatment
epidemiological
characteristics
are
discussed.
Given
variability
transmissibility
MGEs,
continuous
monitoring
control
variation
transmission
such
material
should
prioritized.
Язык: Английский
High-risk clones of carbapenem resistant Klebsiella pneumoniae recovered from pediatric patients in Southern Brazil
Brazilian Journal of Microbiology,
Год журнала:
2024,
Номер
55(2), С. 1437 - 1443
Опубликована: Март 19, 2024
Язык: Английский
Carbapenem-resistant Klebsiella pneumoniae among hospitalized patients in Cape Town, South Africa: molecular epidemiology and characterization
JAC-Antimicrobial Resistance,
Год журнала:
2024,
Номер
6(2)
Опубликована: Март 5, 2024
Abstract
Background
The
molecular
epidemiology
of
carbapenem-resistant
Enterobacterales
in
Cape
Town
remains
largely
unknown.
Objectives
This
study
aimed
to
describe
the
epidemiology,
resistome,
virulome
and
mobilome
Klebsiella
pneumoniae
(CRKP)
within
guide
therapy,
antimicrobial
stewardship
infection
prevention
control
practices.
Methods
Eighty-five
CRKP
isolates
from
hospitalized
patients
underwent
WGS
as
part
a
prospective,
multicentre,
cross-sectional
study,
conducted
between
1
November
2020
30
2022,
across
public-sector
private-sector
hospitals
Town,
South
Africa.
Results
MLST
revealed
three
novel
types,
ST6785,
ST6786
ST6787,
while
most
common
were
ST219,
ST307,
ST17,
ST13
ST2497.
Different
predominant
clones
noted
each
hospital.
carbapenemase
gene
was
blaOXA-48-like,
detected
71%
isolates,
with
blaNDM
5%.
Notably,
co-detection
two
genes
(blaOXA-48-like
blaNDM)
occurred
13%
isolates.
yersiniabactin
siderophore
73%
commonly
associated
ICEKp5
mobile
element.
All
carbapenemases
located
on
plasmids.
blaOXA-181
blaOXA-232
colocalized
ColKP3
replicon
type
assembled
contigs
83%
100%
cases,
respectively.
Conclusions
reflects
institutionally
dominant,
rather
than
regional,
clones.
prevalent
keeping
Africa
general.
Emerging
harbouring
both
blaOXA-48-like
blaNDM,
such
ST2497
are
concern
due
limited
availability
appropriate
agents
Язык: Английский
Computational Systems and Network Biology Perspective: Understanding Klebsiella pneumoniae Infection Mechanisms
The Microbe,
Год журнала:
2024,
Номер
unknown, С. 100175 - 100175
Опубликована: Сен. 1, 2024
Язык: Английский
Klebsiella pneumoniae species complex: From wastewater to the environment
One Health,
Год журнала:
2024,
Номер
19, С. 100880 - 100880
Опубликована: Авг. 17, 2024
Язык: Английский
Emergence of mcr-8.1 -bearing MDR-hypervirulent Klebsiella pneumoniae ST307
Microbiology Spectrum,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 13, 2024
ABSTRACT
We
report
for
the
first
time
whole-genome
sequencing
of
four
multidrug-resistant
sequence
type
(ST)
307
Klebsiella
pneumoniae
recovered
from
patients
in
two
hospitals
Armenia.
Comparative
genomic
analysis
revealed
that
isolates
were
closely
related,
with
a
maximum
39
single
nucleotide
polymorphism
(SNP)
differences
core
genome.
All
Armenian
carried
integrative
and
conjugative
element
ICE
Kp
4,
which
bears
yersiniabactin
locus,
shared
common
evolutionary
origin,
diverging
around
2005
(95%
CI:
1999
to
2011).
Antibiotic
susceptibility
testing
showed
resistance
several
antibiotics,
including
ampicillin,
amoxicillin-clavulanic
acid,
cefepime,
ceftazidime,
norfloxacin,
levofloxacin,
chloramphenicol.
Specifically,
designated
as
ARM03
ARM06
resistant
piperacillin-tazobactam,
ARM04
ARM05
had
intermediate
both
piperacillin-tazobactam
imipenem,
amikacin.
further
identified
antimicrobial
(AMR)
genes
isolates,
bla
OXA-1
,
TEM-1D
SHV-28
dfrA14
tet(A
),
sul2
qnrB1
aac(6´)-Ib-cr
strA
strB
extended-spectrum
β-lactamase
gene
CTX-M-15
.
Additionally,
also
obtained
dfrA5
sul1
sul3
cmlA1
mphA
aph3-Ia
unique
colistin
mcr-8.1
was
absent
all
other
publicly
available
ST307
isolates.
These
acquired
aerobactin
siderophore-encoding
clusters
(
iucABCD-iutA
)
hypermucoidy
locus
rmpADC
(ARM06
rmpA
fragment).
ARM05,
well
ARM06,
nearly
identical
AMR
virulence
genes,
along
similar
plasmid
replicon
profiles,
respectively.
Our
findings
suggest
transmission
event
occurred
between
Armenia,
likely
facilitated
by
or
community
members,
during
K.
plasmids
carrying
genes.
IMPORTANCE
Multidrug-resistant
(MDR)
has
emerged
high-risk
clone
associated
hospital-
community-acquired
infections,
posing
major
threat
global
public
health.
in-depth
comparative
genomics
analyses
The
data
sets.
possessed
incomplete
Armenia
either
through
members.
In
addition,
event.
study
emphasises
importance
surveillance
this
emerging
MDR-hypervirulent
pathogen
provide
early
interventions.
Язык: Английский
The mechanisms of resistance, epidemiological characteristics, and molecular evolution of carbapenem-resistant hypervirulent Klebsiella pneumoniae
Laboratory Medicine,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 23, 2024
Carbapenem-resistant
hypervirulent
Klebsiella
pneumoniae
(CR-hvKP)
is
a
highly
pathogenic,
drug-resistant,
and
transmissible
"superbug"
that
causes
infections
in
hospitals
communities.
Because
of
the
lack
effective
antimicrobial
treatment
options,
morbidity
mortality
from
CR-hvKP
have
increased
dramatically,
outbreaks
rapid
spread
become
major
global
public
health
challenge.
The
mechanisms
molecular
evolution
include
acquisition
plasmid
encoding
virulence
gene
by
carbapenemase-producing
K
pneumoniae,
horizontal
transfer
plasmids
carrying
carbapenem
resistance
genes
to
hvKP,
fusion
both
classic
pneumoniae.
In
addition,
hvKP
can
develop
phenotype
under
antibiotic
pressure.
arises
through
plasmid-mediated
convergence
factors.
Its
multidrug
lethal
pathogenicity
fuel
hospital
outbreaks,
requiring
urgent
action
block
transmission
strengthen
surveillance
contain
this
evolving
superbug.
article,
we
summarized
carbapenemase
mechanism,
factors,
epidemiology
CR-hvKP.
Our
aim
was
elucidate
evolutionary
mechanism
provide
reference
for
curbing
Язык: Английский