COMPARATIVE CHARACTERISTICS OF THE ACCUMULATION OF DIFFERENT VARIANTS OF THE SARS-COV-2 VIRUS (WUHAN, DELTA, OMICRON) IN THE ORGANS OF MODEL ANIMALS

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 13, 2024

ABSTRACT Background The genotypic variability of the SARS-CoV-2 virus has proven to be extremely high, and emergence new strains raises concerns about their possible high virulence, transmissibility, ability bypass responses body’s immune system induced by previous infection or vaccination. Therefore, one main tasks is study pathogenesis various variants using experimental animal biomodels quickly find methods approaches fighting viruses. Methods 60 humanized mice C57BL/6-Tgtn (CAG-human AEC2-IRES-Luciferase-WPRE-polyA) line (hACE2) were used. Mice infected intranasally at different doses with three virus: Wuhan, Delta Omicron. Results We showed that hACE2 mice, when all virus, typical pathological changes in lung consistency comparable those found COVID-19 humans. All developed interstitial pneumonia, characterized inflammatory cell infiltration thickening alveolar septa, characteristic vascular damage. Conclusions At a dose 4 lg plaque-forming unit (PFU), 100% mortality. A dose-dependent effect was established only for Wuhan variants. In comparative assessment mouse model hACE2, it variant leads more severe damage compared

Язык: Английский

Synthesis of Liposomes Conjugated with CpG-Oligonucleotide and Loaded with a Set of T-Cell Epitopes of the SARS-CoV-2 Virus

Russian Journal of Bioorganic Chemistry, Год журнала: 2023, Номер 49(4), С. 905 - 911

Опубликована: Авг. 1, 2023

Язык: Английский

Synthesis of Liposomes Conjugated with CpG Oligonucleotide and Loaded with a Set of T-Cell Epitopes of the SARS-CoV-2 Virus

Биоорганическая химия, Год журнала: 2023, Номер 49(4), С. 434 - 440

Опубликована: Июль 1, 2023

Язык: Английский