Single-Cell RNA-Seq Reveals the Promoting Role of Ferroptosis Tendency During Lung Adenocarcinoma EMT Progression DOI Creative Commons
Jiaxi Yao, Yuchong Zhang, Mengling Li

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2022, Номер 9

Опубликована: Янв. 20, 2022

Epithelial-mesenchymal transition (EMT) and ferroptosis are two important processes in biology. In tumor cells, they intimately linked. We used single-cell RNA sequencing to investigate the regulatory connection between EMT tendency LUAD epithelial cells. Seurat construct expression matrix using GEO dataset GSE131907 extract found a positive correlation trends of tendency. Then we SCENIC analyze differentially activated transcription factors constructed molecular directed network by causal inference. Some markers (GPX4, SCP2, CAV1) were have strong effects on EMT. Cell communication networks iTALK implied that Ferro_High_EMT_High cells higher SDC1, SDC4, activation LGALS9-HARVCR2 pathways. By deconvolution bulk sequencing, results CIBERSORTx showed co-occurrence may lead metastasis non-response immunotherapy. Our findings there is Ferroptosis promotive effect High propensities poor prognosis

Язык: Английский

Suppression of lncRNA Gm47283 attenuates myocardial infarction via miR-706/ Ptgs2/ferroptosis axis DOI Open Access
Feng Gao,

Yongcheng Zhao,

Bin Zhang

и другие.

Bioengineered, Год журнала: 2022, Номер 13(4), С. 10786 - 10802

Опубликована: Апрель 1, 2022

Myocardial infarction (MI) is the leading cause of sudden death. Long non-doing RNAs (lncRNAs) were demonstrated to play crucial roles in multiple diseases, including cancer and cardiovascular diseases. Nevertheless, molecular mechanism lncNRAs MI unclear. In this study, we integrated bioinformatics biological experiments identify novel lncRNA transcripts elucidated its regulatory MI. First, identified 10 dysregualted lncRNAs found that Gm47283 was top risk factor Bioinformatics analysis predicted exerted function via targeting miR-706 Ptgs2. Ptgs2 also known regulator ferroptosis. Inhibition or overexpression could regulate expression downstream ferroptosis activity. Overexpression inhibit activity ferroptosis, thereby attenuated cellular injury. Mechanically, co-transfection showed reverse damage effect caused by overexpression, inhibiting Additionally, inhibition stem cell membrane coated siRNA attenuate vivo. Our study a containing Gm47283/miR-706/Ptgs2/ferroptosis MI, which provided potential therapeutic for MI.Graphical Abstract. Stem inhibits cardiomyocyte myocardial rat. membrane-coated increases miR-706, then suppresses reduce lipid peroxidation toxicity, PUFA: polyunsaturated fatty acid.

Язык: Английский

Процитировано

43
Targeting stearoyl-coa desaturase enhances radiation induced ferroptosis and immunogenic cell death in esophageal squamous cell carcinoma DOI Creative Commons
Hui Luo, Xiaohui Wang, Shuai Song

и другие.

OncoImmunology, Год журнала: 2022, Номер 11(1)

Опубликована: Июль 15, 2022

Overcoming resistance to radiation is a major challenge in cancer treatment. Stearoyl-coa desaturase (SCD1) the enzyme responsible for oleic acid (OA) and palmitoleic (POA) formation. Here, we provided evidence that targeting SCD1 was capable of inducing ferroptosis immunogenic cell death (ICD), thereby improving sensitivity esophageal squamous carcinoma (ESCC). ESCC lines with high expression were treated MF-438 (SCD1 inhibitor) determine viability. Colony formation assay performed evaluate sensitization inhibitor. Tumor ICD analyzed MF-438, therapy (RT) combination treatment group. The potential molecular mechanisms underlying as novel sensitizer explored. We concluded by assessing prognostic factor ESCC. exhibited antitumor activity cells. Our outcomes revealed significant improvement MF-438. Moreover, enhanced tumor ICD. Further analyses conferred via alleviating cells; inhibited biosynthesis OA POA, improved induced Clinical analysis indicated associated unfavorable survival patients In summary, our results demonstrated acted inducer. Targeting immunogenicity ferroptotic cells increased effectiveness RT could be considered useful indicator

Язык: Английский

Процитировано

43
The crosstalk between ferroptosis and anti‐tumor immunity in the tumor microenvironment: molecular mechanisms and therapeutic controversy DOI Creative Commons
Yichen Zheng, Lingqi Sun,

Jiamin Guo

и другие.

Cancer Communications, Год журнала: 2023, Номер 43(10), С. 1071 - 1096

Опубликована: Сен. 17, 2023

The advent of immunotherapy has significantly reshaped the landscape cancer treatment, greatly enhancing therapeutic outcomes for multiple types cancer. However, only a small subset individuals respond to it, underscoring urgent need new methods improve its response rate. Ferroptosis, recently discovered form programmed cell death, emerged as promising approach anti-tumor therapy, with targeting ferroptosis kill tumors seen potentially effective strategy. Numerous studies suggest that inducing can synergistically enhance effects immunotherapy, paving way combined treatment method in future. Nevertheless, recent research raised concerns about potential negative impacts on immunity consequence ferroptosis, leading conflicting views within scientific community interplay between and immunity, thereby necessity comprehensive review existing literature this relationship. Previous reviews have touched related content, many focusing primarily promoting role while overlooking evidence inhibitory immunity. Others concentrated solely discussing content either from perspective cells or immune ferroptosis. Given both exist tumor microenvironment, one-sided discussion cannot comprehensively summarize topic. Therefore, perspectives tumor-infiltrating cells, we systematically current intending provide explanations identify work needed establish translational basis ferroptosis-targeted therapy treating tumors.

Язык: Английский

Процитировано

42
The amino acid transporter SLC7A11-mediated crosstalk implicated in cancer therapy and the tumor microenvironment DOI

Jiaqin He,

Xiaomeng Wang, Keying Chen

и другие.

Biochemical Pharmacology, Год журнала: 2022, Номер 205, С. 115241 - 115241

Опубликована: Сен. 6, 2022

Язык: Английский

Процитировано

41
Combination of ferroptosis and pyroptosis dual induction by triptolide nano-MOFs for immunotherapy of Melanoma DOI Creative Commons

Shengmei Wang,

Qiuyan Guo,

Rubing Xu

и другие.

Journal of Nanobiotechnology, Год журнала: 2023, Номер 21(1)

Опубликована: Окт. 19, 2023

Abstract Immunotherapy has good potential to eradicate tumors in the long term. However, due low immunogenicity of tumor cells, current cancer immunotherapies are not effective. To address this limitation, we constructed a BSA-FA functionalized iron-containing metal-organic framework (TPL@TFBF) that triggers potent systemic anti-tumor immune response by inducing ferroptosis and pyroptosis cells releasing large quantities damage-associated molecular patterns (DAMPs) induce immunogenicity, showing excellent efficacy against melanoma lung metastases vivo. This nanoplatform forms through coordination between tannic acid (TA) Fe 3+ is then loaded with triptolide (TPL), which coated FA-modified BSA. The nanoparticles target FA modification, TPL, TA. reduced 2+ TA, triggering Fenton reaction resulting ROS production. Moreover, TPL increases production intracellular inhibiting expression nuclear factor erythroid-2 related (Nrf2). Such simultaneous amplification induces undergo pyroptosis, amounts DAMPs, stimulate antigen presentation dendritic (DCs) proliferation cytotoxic T lymphocytes (CD4+/CD8 + cells) inhibit metastasis. In addition, combining nanoparticle treatment checkpoint blockade (ICB) further inhibits growth. work provides new strategy for immunotherapy based on various combinations cell death mechanisms.

Язык: Английский

Процитировано

40
Understanding sorafenib-induced ferroptosis and resistance mechanisms: Implications for cancer therapy DOI
Qiuhong Li, Kexin Chen, Tianyi Zhang

и другие.

European Journal of Pharmacology, Год журнала: 2023, Номер 955, С. 175913 - 175913

Опубликована: Июль 17, 2023

Язык: Английский

Процитировано

37
Mitochondrial-targeted brequinar liposome boosted mitochondrial-related ferroptosis for promoting checkpoint blockade immunotherapy in bladder cancer DOI

Qiubo Ding,

Wenfu Tang, Xianglong Li

и другие.

Journal of Controlled Release, Год журнала: 2023, Номер 363, С. 221 - 234

Опубликована: Сен. 28, 2023

Язык: Английский

Процитировано

37
Macrophage-inherited exosome excise tumor immunosuppression to expedite immune-activated ferroptosis DOI Creative Commons
Duo Wang, Guanhua Qiu, Xiaoqi Zhu

и другие.

Journal for ImmunoTherapy of Cancer, Год журнала: 2023, Номер 11(5), С. e006516 - e006516

Опубликована: Май 1, 2023

Immunosuppressive tumor microenvironment (ITM) remains an obstacle that jeopardizes clinical immunotherapy.

Язык: Английский

Процитировано

34
Spatiotemporal local and abscopal cell death and immune responses to histotripsy focused ultrasound tumor ablation DOI Creative Commons

Ashley Pepple,

Joey L. Guy,

Reliza McGinnis

и другие.

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Янв. 23, 2023

Introduction Histotripsy is a novel focused ultrasound tumor ablation modality with potent immunostimulatory effects. Methods To measure the spatiotemporal kinetics of local andabscopal responses to histotripsy, C57BL/6 mice bearing bilateral flank B16 melanoma or Hepa1-6 hepatocellular carcinoma tumors were treated unilateral sham partial histotripsy. Treated and contralateral untreated (abscopal) analyzed using multicolor immunofluorescence, digital spatial profiling, RNA sequencing (RNASeq), flow cytometry. Results Unilateral histotripsy triggered abscopal growth inhibition. Within zone, early high mobility group box protein 1 (HMGB1) release necroptosis accompanied by immunogenic cell death transcriptional in cells innate immune activation infiltrating myeloid natural killer (NK) cells. Delayed CD8+ T intratumoral infiltration was spatiotemporally aligned cancer features ferroptosis; this effect enhanced CTLA-4 blockade recapitulated vitro when tumor-draining lymph node co-cultured Inoculation cell-free fractions generated but not radiation freeze/thaw conferred protection from challenge. Discussion We propose that may evoke necroptotic death, priming systemic adaptive ferroptotic death.

Язык: Английский

Процитировано

30
From synergy to resistance: Navigating the complex relationship between sorafenib and ferroptosis in hepatocellular carcinoma DOI Creative Commons
Zijian Wang, Chunyang Zhou, Yiming Zhang

и другие.

Biomedicine & Pharmacotherapy, Год журнала: 2023, Номер 170, С. 116074 - 116074

Опубликована: Дек. 25, 2023

Hepatocellular carcinoma (HCC) remains a major global health burden, and sorafenib, multi-kinase inhibitor, has shown effectiveness in the treatment of HCC is considered as first-line therapy for advanced HCC. However, response to sorafenib varies among patients, development drug resistance poses prevalent obstacle. Ferroptosis, newly characterized form cell death featured by iron-dependent lipid peroxidation, emerged critical player reaction The induction ferroptosis been augment anticancer benefits sorafenib. it also observed contribute resistance. This review presents comprehensive thorough analysis that elucidates intricate relationship between over recent years, aiming formulate effective therapeutic approaches liver cancer. Based on this exploration, we propose innovative strategies intended overcome via targeted modulation ferroptosis.

Язык: Английский

Процитировано

25