Impact of lymph node metastasis on immune microenvironment and prognosis in colorectal cancer liver metastasis: insights from multiomics profiling

British Journal of Cancer, Год журнала: 2025, Номер unknown

Опубликована: Янв. 3, 2025

Abstract Background This study aimed to investigate the prognostic impact of lymph node metastasis (LNM) on patients with colorectal cancer liver (CRLM) and elucidate underlying immune mechanisms using multiomics profiling. Methods We enrolled CRLM from US Surveillance, Epidemiology, End Results (SEER) cohort a multicenter Chinese cohort, integrating bulk RNA sequencing, single-cell sequencing proteomics data. The cancer-specific survival (CSS) profiles tumor-draining nodes (TDLNs), primary tumors were compared between without LNM. Pathological evaluations used assess cell infiltration histological features. LNM had significantly shorter CSS than in two large cohorts. Our results showed that nonmetastatic TDLNs exhibited greater abundance cells, including CD4+ T CD8+ CD19+ B whereas metastatic enriched fibroblasts, endothelial macrophages. Immunohistochemical analysis confirmed elevated levels CD3+ cells TDLNs. presence was associated enhanced antitumor responses tumors, characterized by higher Klintrup–Makinen (KM) grade tertiary lymphoid structures. Furthermore, predominantly desmoplastic growth pattern (dHGP), while those replacement (rHGP). Conclusions research highlights adverse reveals potential related through analysis. paves way for further refinement AJCC TNM staging system clinical practice.

Язык: Английский

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Tertiary Lymphoid Structures as a Biomarker in Immunotherapy and Beyond: Advancing Towards Clinical Application

Cancer Letters, Год журнала: 2025, Номер unknown, С. 217491 - 217491

Опубликована: Янв. 1, 2025

Язык: Английский

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Tertiary lymphoid structures achieve ‘cold’ to ‘hot’ transition by remodeling the cold tumor microenvironment

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Год журнала: 2025, Номер 1880(3), С. 189312 - 189312

Опубликована: Апрель 6, 2025

Язык: Английский

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TCF19/CDKN2A Regulates Glycolysis and Macrophage M2 Polarization for Osteosarcoma Progression

The FASEB Journal, Год журнала: 2025, Номер 39(7)

Опубликована: Апрель 7, 2025

ABSTRACT Osteosarcoma (OS) is the most common malignant tumor of bone. This paper aimed to explore mechanism macrophage polarization and glycolysis in OS. Gene expression microarray GSE42572 for OS was downloaded from GEO database validated TCGA‐SARC. CDKN2A cell lines normal human bone osteoblasts detected. Saos2 cells were transfected with siRNA‐CDKN2A, U2OS pcDNA3.4‐CDKN2A knock down or upregulate role behaviors. Extracellular acidification rate, oxygen consumption glycolysis‐related proteins co‐incubated THP‐1 cells, CD206 CD86 levels The secretion IL‐10 IL‐12 by macrophages measured. upstream regulatory elements predicted online databases, binding TCF19 promoter validated. Xenograft established verify effect knockdown on growth mice. highly expressed tissues lines. inhibited proliferation, migration, invasion promoted apoptosis glycolysis. After co‐incubation macrophages, CD206‐positive decreased, CD86‐positive increased, increased. enriched expression. Upregulation M2 polarization. downregulation growth, metabolic reprogramming, enhances its expression, which turn activates polarization, ultimately promoting progression.

Язык: Английский

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A novel immunological perspective on female-specific cancers: Exploring the signaling pathways of tertiary lymphoid structures and their clinical applications

Life Sciences, Год журнала: 2025, Номер 377, С. 123800 - 123800

Опубликована: Июнь 4, 2025

Язык: Английский

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