Unveiling the Genomic Features and Biocontrol Potential of Trichoderma hamatum Against Root Rot Pathogens

Journal of Fungi, Год журнала: 2025, Номер 11(2), С. 126 - 126

Опубликована: Фев. 8, 2025

Fusarium species are among the most significant pathogens causing root rot in Panax notoginseng. In this study, a strain of Trichoderma hamatum was isolated from rhizosphere soil P. notoginseng and subjected to whole-genome sequencing. Plate confrontation experiments were conducted investigate antagonistic effects T. against oxysporum, solani, acutatum, primary rot. Whole-genome sequencing revealed 10,774 predicted genes hamatum, which 454 associated with carbohydrate-active enzymes (CAZymes) involved fungal cell wall degradation. Additionally, 11 biosynthetic gene clusters (BGCs) antimicrobial production identified, highlighting biocontrol potential hamatum. plate experiments, showed substantial inhibition rates 68.07%, 70.63%, 66.12% F. respectively. Scanning electron microscopy suggested hyperparasitism characterized by spore that adhered pathogen, thereby inhibiting its growth. These findings provide theoretical foundation enhance understanding biological control mechanisms supporting applications sustainable agriculture.

Язык: Английский

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Decrypting biocontrol functions and application modes by genomes data of three Trichoderma Strains/Species

Fungal Genetics and Biology, Год журнала: 2024, Номер 172, С. 103889 - 103889

Опубликована: Март 20, 2024

Язык: Английский

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Lifestyle-specific responses ofTrichodermaspp. in mycoparasitic confrontations and implications for biocontrol ofPopulusxcanescens

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 17, 2024

Summary Trichoderma spp. are gaining popularity in agriculture and forestry due to their multifaceted roles promoting plant growth through e.g. nutrient translocation, hormone production, induction of systemic resistance, but also direct antagonism other fungi. However, the mycotrophic nature genus bears risk possible interference with native plant-beneficial fungi, such as ectomycorrhiza, rhizosphere. Such could yield unpredictable consequences for host plants these ecosystems. We investigated whether can differentiate between beneficial ectomycorrhizal fungi (represented by Laccaria bicolor Hebeloma cylindrosporum ) pathogenic Fusarium graminearum Alternaria alternata different confrontation scenarios, including a newly developed olfactometer “race tube”-like system. Using two independent species, T. harzianum, atrobrunneum , plant-growth-promoting immune-stimulating properties towards Populus x canescens our study revealed robustly accelerated phytopathogens, while showing contrary response Transcriptomic analyses identified distinct genetic programs during interaction corresponding lifestyles, emphasizing expression mycoparasitism-related genes only presence phytopathogens. The findings reveal critical mode fungal community interactions belowground suggest that distinguish partners lifestyles already at distance.

Язык: Английский

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The decision for or against mycoparasitic attack by Trichoderma spp. is taken already at a distance in a prey-specific manner and benefits plant-beneficial interactions

Fungal Biology and Biotechnology, Год журнала: 2024, Номер 11(1)

Опубликована: Сен. 9, 2024

Язык: Английский

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Transcriptome Sequencing and Analysis of Trichoderma polysporum Infection in Avena fatua L. Leaves before and after Infection

Journal of Fungi, Год журнала: 2024, Номер 10(5), С. 346 - 346

Опубликована: Май 13, 2024

Biological control is a scientific management method used in modern agricultural production, and microbially derived biopesticides are one effective with which to weeds fields. In order determine the key genes for weed by Trichoderma polysporum, transcriptome sequencing was carried out high-throughput technology, strains of T. polysporum HZ-31 infesting Avena fatua L. at 24, 48, 72 h were as experimental group, 0 group. A total 690,713,176 clean reads obtained, results each group (0 h) analyzed. total, 3464 differentially expressed found after 24 infection pathogen, including 1283 down-regulated 2181 up-regulated genes. After 48 infection, number 3885, 2242 1643 down-regulated. The highest among all groups, 4594 genes, 2648 1946 analyzed GO KEGG, showed that mainly enriched biosynthesis phenylalanine, tyrosine, tryptophan; degradation aromatic compounds; methane metabolism; other pathways. Among them, PHA2, GDH, ADH2, AROF significantly above-mentioned pathways, so they hypothesized play an important role synthesis herbicidally active substances HZ-31. this study can provide theoretical basis further studies on pathogenicity A. L., accelerate development utilization new efficient bioherbicides.

Язык: Английский

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Genomic Characterization and Establishment of a Genetic Manipulation System for Trichoderma sp. (Harzianum Clade) LZ117

Journal of Fungi, Год журнала: 2024, Номер 10(10), С. 697 - 697

Опубликована: Окт. 7, 2024

species have been reported as masters in producing cellulolytic enzymes for the biodegradation of lignocellulolytic biomass and biocontrol agents against plant pathogens pests. In our previous study, a novel

Язык: Английский

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