Dihydroartemisinin attenuates PM-induced lung injury by inhibiting inflammation and regulating autophagy DOI Creative Commons
Lingjing Liu, Jing Li,

Yincong Xue

и другие.

Frontiers in Public Health, Год журнала: 2025, Номер 13

Опубликована: Март 7, 2025

Objective The study investigates the effects and mechanisms of dihydroartemisinin (DHA) in mitigating lung injury induced by particulate matter (PM). Methods model was PM particles vivo vitro . Hematoxylin Eosin (H&E) staining utilized for detection thickening airway wall infiltration inflammatory cells mouse tissue. expressions factors were detected alveolar lavage fluid cell supernatant. TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) staining, Caspase-1, Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), microtubule-associated 1 light chain 3-II (LC3-II) Belcin-1 used to observe apoptosis autophagy related tissue, p-p65 immunofluorescence. Results H&E revealed DHA alleviates PM-induced Moreover, reduced IL-6, IL-8, IL-1β levels ~50% ( p < 0.05), highlighting its anti-inflammatory effects. Furthermore, immunohistochemistry showed that treatment inhibited pro-apoptotic expression Bax/BCL2 cleaved-Caspase-3, respectively. In addition, immunofluorescence LC3-II Beclin-1 dramatically increased group compared Control group, but greatly DHA. Further, we found activation NF-KB signaling pathway. Conclusion protects against through anti-inflammatory, anti-apoptotic, autophagy-regulating mechanisms, offering a potential drug option improving injury.

Язык: Английский

Dihydroartemisinin attenuates PM-induced lung injury by inhibiting inflammation and regulating autophagy DOI Creative Commons
Lingjing Liu, Jing Li,

Yincong Xue

и другие.

Frontiers in Public Health, Год журнала: 2025, Номер 13

Опубликована: Март 7, 2025

Objective The study investigates the effects and mechanisms of dihydroartemisinin (DHA) in mitigating lung injury induced by particulate matter (PM). Methods model was PM particles vivo vitro . Hematoxylin Eosin (H&E) staining utilized for detection thickening airway wall infiltration inflammatory cells mouse tissue. expressions factors were detected alveolar lavage fluid cell supernatant. TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) staining, Caspase-1, Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), microtubule-associated 1 light chain 3-II (LC3-II) Belcin-1 used to observe apoptosis autophagy related tissue, p-p65 immunofluorescence. Results H&E revealed DHA alleviates PM-induced Moreover, reduced IL-6, IL-8, IL-1β levels ~50% ( p < 0.05), highlighting its anti-inflammatory effects. Furthermore, immunohistochemistry showed that treatment inhibited pro-apoptotic expression Bax/BCL2 cleaved-Caspase-3, respectively. In addition, immunofluorescence LC3-II Beclin-1 dramatically increased group compared Control group, but greatly DHA. Further, we found activation NF-KB signaling pathway. Conclusion protects against through anti-inflammatory, anti-apoptotic, autophagy-regulating mechanisms, offering a potential drug option improving injury.

Язык: Английский

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