Signal Transduction and Targeted Therapy,
Год журнала:
2020,
Номер
5(1)
Опубликована: Сен. 8, 2020
Abstract
Drug
resistance
is
a
major
hurdle
in
cancer
treatment
and
key
cause
of
poor
prognosis.
Epitranscriptomics
epiproteomics
are
crucial
cell
proliferation,
migration,
invasion,
epithelial–mesenchymal
transition.
In
recent
years,
epitranscriptomic
epiproteomic
modification
has
been
investigated
on
their
roles
overcoming
drug
resistance.
this
review
article,
we
summarized
the
progress
three
novel
aspects:
(i)
mRNA
modification,
which
includes
alternative
splicing,
A-to-I
methylation;
(ii)
noncoding
RNAs
involves
miRNAs,
lncRNAs,
circRNAs;
(iii)
posttranslational
molecules
encompasses
inactivation/efflux,
target
modifications,
DNA
damage
repair,
death
resistance,
EMT,
metastasis.
addition,
discussed
therapeutic
implications
targeting
some
classical
chemotherapeutic
drugs
such
as
cisplatin,
5-fluorouridine,
gefitinib
via
these
modifications.
Taken
together,
highlights
importance
provides
new
insights
potential
targets
to
reverse
Frontiers in Genetics,
Год журнала:
2019,
Номер
10
Опубликована: Апрель 5, 2019
Single-cell
RNA
sequencing
(scRNA-seq)
technologies
allow
the
dissection
of
gene
expression
at
single-cell
resolution,
which
greatly
revolutionizes
transcriptomic
studies.
A
number
scRNA-seq
protocols
have
been
developed,
and
these
methods
possess
their
unique
features
with
distinct
advantages
disadvantages.
Due
to
technical
limitations
biological
factors,
data
are
noisier
more
complex
than
bulk
RNA-seq
data.
The
high
variability
raises
computational
challenges
in
analysis.
Currently,
although
an
increasing
bioinformatics
proposed
for
analyzing
interpreting
data,
novel
algorithms
required
ensure
accuracy
reproducibility
results.
In
this
review,
we
provide
overview
currently
available
isolation
technologies,
discuss
diverse
analyses
including
quality
control,
read
mapping,
quantification,
batch
effect
correction,
normalization,
imputation,
dimensionality
reduction,
feature
selection,
cell
clustering,
trajectory
inference,
differential
calling,
alternative
splicing,
allelic
regulatory
network
reconstruction.
Further,
outline
prospective
development
applications
technologies.
Epithelial-to-mesenchymal
transition
(EMT)
is
associated
with
metastasis
formation
as
well
generation
and
maintenance
of
cancer
stem
cells.
In
this
way,
EMT
contributes
to
tumor
invasion,
heterogeneity
chemoresistance.
Morphological
functional
changes
involved
in
these
processes
require
robust
reprogramming
gene
expression,
which
only
partially
accomplished
at
the
transcriptional
level.
Alternative
splicing
another
essential
layer
expression
regulation
that
expands
cell
proteome.
This
step
post-transcriptional
tightly
controls
identity
between
epithelial
mesenchymal
states
during
differentiation.
Importantly,
dysregulation
factor
function
cancer-specific
isoform
frequently
occurs
human
tumors,
suggesting
importance
alternative
for
biology.
review,
we
briefly
discuss
role
programs
development,
differentiation
progression.
Next,
focus
on
selected
examples
key
factors
are
regulated
post-transcriptionally
through
mechanisms.
Lastly,
describe
relevant
oncogenic
splice-variants
directly
orchestrate
biology
EMT,
may
be
envisioned
novel
targets
therapeutic
intervention.
The Annual Review of Pharmacology and Toxicology,
Год журнала:
2019,
Номер
59(1), С. 537 - 554
Опубликована: Янв. 6, 2019
Metal
exposure
is
pervasive
and
not
limited
to
sporadic
poisoning
events
or
toxic
waste
sites.
Hundreds
of
millions
people
around
the
globe
are
affected
by
chronic
metal
exposure,
which
associated
with
serious
health
concerns,
including
cancer,
as
demonstrated
in
a
variety
studies
at
molecular,
systemic,
epidemiologic
levels.
Metal-induced
toxicity
carcinogenicity
sophisticated
complex
nature.
This
review
provides
broad
context
holistic
view
currently
available
on
mechanisms
metal-induced
carcinogenesis.
Specifically,
we
focus
five
most
prevalent
carcinogenic
metals,
arsenic,
nickel,
cadmium,
chromium,
beryllium,
their
potential
drive
carcinogenesis
humans.
A
comprehensive
understanding
behind
development
cancer
can
provide
valuable
insights
for
therapeutic
intervention
involving
molecular
targets
International Journal of Molecular Sciences,
Год журнала:
2017,
Номер
18(8), С. 1652 - 1652
Опубликована: Июль 29, 2017
In
the
last
decades,
transcriptome
profiling
has
been
one
of
most
utilized
approaches
to
investigate
human
diseases
at
molecular
level.
Through
expression
studies,
many
biomarkers
and
therapeutic
targets
have
found
for
several
pathologies.
This
number
is
continuously
increasing
thanks
total
RNA
sequencing.
Indeed,
this
new
technology
completely
revolutionized
analysis
allowing
quantification
gene
levels
allele-specific
in
a
single
experiment,
as
well
identify
novel
genes,
splice
isoforms,
fusion
transcripts,
world
non-coding
an
unprecedented
sequencing
also
employed
important
projects,
like
ENCODE
(Encyclopedia
regulatory
elements)
TCGA
(The
Cancer
Genome
Atlas),
provide
snapshot
dozens
cell
lines
thousands
primary
tumor
specimens.
Moreover,
these
studies
paved
way
development
data
integration
order
facilitate
management
disease
markers
use
clinics.
scenario,
ongoing
clinical
trials
utilize
through
strategies
instrument
diagnosis
numerous
